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Evaluation of antimicrobial and cytotoxic properties of Leucas aspera and Spilanthes paniculata

M Alam Morshed, Azim Uddin, Rahman Saifur, Anik Barua, Anwarul Haque

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Int. J. Biosci.1(2), 7-16, April 2011


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Plants are rich source of antibacterial agents, which could be exploited in human disease management. The main aim of this study was to find out the antibacterial and cytotoxic activity of the n-hexane, ethyl acetate and ethanol extract of Leucas aspera and the ethanol fraction of chloroform, methanol and ethanol extract of Spilanthes paniculata were studied. The fraction and extracts of S. paniculata showed potentialantimicrobial activities against 14 strains of microorganisms whereas L. aspera did not show any antimicrobial response. For antimicrobial test, Disc diffusion technique was used against five Gram positive and eight Gram negative bacteria and one fungi. The zone of inhibition of microorganisms was measured in mm. In vitro cytotoxicity test was also studied with both of the plants by Brine Shrimp Lethality Bioassay and results illustrated significant (p<0.05) cytotoxicity against A. salina, that were expressed as LC50.


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Evaluation of antimicrobial and cytotoxic properties of Leucas aspera and Spilanthes paniculata

Balick JM, Cox PA. 1996. Plants, People and Culture: the Science of Ethnobotany, Scientific American Library, New York, 228.

Cotton CM. 1996. Ethnobotany: Principle and Application. John Wiley and Sons, New York, 399.

Nair R, Chanda S. 2006. Activity of some medicinal plants against certain pathogenic bacterial strains. Indian J. Pharmacol 38, 142-144.

Nascimento GGF, Locatelli J, Freitas PC, Silva GL. 2000. Antibacterial activity of plant extracts and phytochemicals on antibiotic-resistant bacteria. Braz. J. Microbiol 31, 247-256.

Greathead W. 2003. Extraction and Antimicrobial Effect of Thai Herbs and Spices Extracts, J. Agricultural and Food Chemistry 43, 361-371.

Kirtikar KR, Basu BD. 1991. In: Indian Medicinal Plants. Vol III, Deharadun: Lalit Mohan Basu, 2019.

Reddy KM, Viswanathan S, Thirugnanasabmantham D, Santa R, Lalitha K. 1993. Analgesic activity of L. aspera. Fitoterapia 64, 151-154

Jansen RK. 1985. The systematics of Acmella (Asteraceae- Heliantheae). Syst. Bot. Monogr 8, 1-115.

Pandey V, Agrawal V, Raghavendra K, Dash AP. 2007. Strong larvicidal activity of three species of Spilanthes (Akarkara) against malaria (Anopheles stephensi Liston, Anopheles culicifacies, Species C) and filaria vector (Culex quinquefasciatus Say). Parasitol. Res. 102, 171–174

Prachayasittikul S, Suphapong S, Worachartcheewan A, Lawung R, Ruchirawat S, Prachayasittikul V. 2009. Bioactive Metabolites from Spilanthes acmella Murr. Molecules 14, 850–867.

Nakatani N, Nagashima M. 1992. Pungent alkamides from Spilanthes acmella L. var. oleracea Clarke. Biosci Biotechnol Biochem. 56, 759–762

Sittie AA, Lemmich E, Olsen CE, Hviid L, Broegger Christensen S. 1998. Alkamides from Phyllanthus fraternus. Planta Med. 64, 192–193

Majumder M, Shukla AK, Arunachalam A. 2008. Nutrient release and fungal succession during decomposition of weed residues in a shifting cultivation system. Communications in Biometry and Crop Science 3 (1), 45–59.

Haque M, Haque ME,  Rahman  MM.  2008. Antibacterial and cytotoxic activities of Capparis zeylanica Linn roots. Ars. Pharmaceutica 49, 31-37

Bauer AW, Kirby WMM, Sherries JC, Tuck M. 1966. Antibiotic susceptibility testing by a standardized disc diffusion method. American Journal of Clinical Pathology 45, 493-496

Meyer BN, Ferrigni NR, Putnam JE, Jacobsen JB, D.E. Nicholsand, Mclaughlin JL. 1982. Brine shrimp; a convenient general bioassay for activeplant constituents. Planta Medica 45, 31-34.

Aguinaldo AM, Espeso EI, Guevara BQ, Nonato MG. 2005. Phytochemistry. In: Guevara BQ (ed.) A Guidebook to Plant Screening: Phytochemical and Biological. University of Santo Tomas, Manila, Philippines.

Moon YJ, Wang X, Morris ME. 2006. Dietary flavonoids: Effects on xenobiotic and carcinogen metabolism. Toxicology 20, 187-210.

Veitch NC. 2007. Isoflavonoids of the Leguminosae. Nat. Prod. Rep. 24, 417-464.

Jiang H, Zhan WQ, Liu X, Jiang SX. 2008. Antioxidant activities of extracts and flavonoid compounds from Oxytropis falcate Bunge. Nat. Prod. Res. 22(18), 1650-1656.

Kim HP, Son KH, Chang HW, Kang SS. 2004. Anti-inflammatory Plant Flavonoids and Cellular Action Mechanisms. J. Phamacol. Sci. 96, 229-245.

Wu JH, Tung YT, Chien SC, Wang SY, Kuo YH, Shyur LF, Chang ST. 2008. Effect of Phytocompounds from the Heart-wood of Acacia confusa on Inflammatory Mediator Production. J. Agric. Food Chem. 56, 1567-1573.

Dzubak P, Hajduch M, Vydra D, Hustova A, Kvasnica M, Biedermann D, Markova L, Urba M, Sarek J. 2006. Pharmacological  activities  of natural triterpenoids and their therapeutic implications. Nat. Prod. Rep. 23, 394-411.

Mohana DC, Satish S, Raveesha KA. 2008. Antibacterial Evaluation of Some Plant Extracts Against Some Human Pathogenic Bacteria. Advances in Biological Research 2(3-4), 49-55.

Chowdhury SA, Sohrab MH, Haque MR, Choudhury MH, Rashid MA. 2008. Phytochemical and biological investigations of Polygonum lanatum. Orien. Pharm. Exp. Med. 8(1), 97-102

McLaughlin J, Chang C, Smith D. 1991. Proceedings of the 18th National Seminar and Unesco Workshop on Natural Products.Institute of Advance Studies, University of Malaysia, Malaysia.

Meyer BN, Ferrigni NR, Putnam JE, Jacobsen LB, Nichols DE, McLaughlin JL. 1982. Brine Shrimp: A convenient  general  bioassay for active plant constituents. Planta Med. 45, 31-34.

Sam TW. 1993.  Toxicity  testing  using  the  brine shrimp: Artemia salina. In: Colgate SM, Molyneux RJ (eds.). Bioactive Natural Products:Detection, Isolation and Structural Determination. CRC Press, USA.

Rahman  MS,  Sadhu  SK,  Hasan  CM.  2007. Preliminary antinociceptive, antioxidant and cytotoxic activities of Leucas aspera root. Fitoterapia 78 (7-8), 552-555.


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