Evaluation of applicability of CETP gene mutation as the predictor of cardiovascular disease in Bangladeshi population

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Research Paper 07/04/2023
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Evaluation of applicability of CETP gene mutation as the predictor of cardiovascular disease in Bangladeshi population

Md. Mahbubur Rahman, Ratna Khatun, Md. Jahangir Alam, Farhana Yesmin, Masuma Hossina Jarin, Md. Mahmudul Hasan Maruf, Md. Zahidus Sayeed, Md. Anwarul Kabir Bhuiya, Md. Abu Reza
Int. J. Biosci.22( 4), 64-71, April 2023.
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Cholesterol ester transfer protein (CETP) is a hydrophobic glycoprotein that mediates the transfer of cholesteryl ester from HDL in exchange for triglycerides in apolipoprotein B100 containing lipoproteins. Several studies showed genetic mutation in different populations in the CETP gene contributes to the manifestation of cardiovascular diseases (CVD) in that population. Exon 15 is the hotspot of CETP gene mutation. Therefore, the aim of the present study is to search for genetic mutation of CETP gene at exon 15 in the Bangladeshi population. In this study, total of 200 subjects including 100 CVD patients were recruited. Blood samples were drawn from the participants for the assessment of total cholesterol, LDL, HDL and triglycerides. Socio-demographic analyses were carried out among the CVD patients and control. DNA was extracted from the blood sample of selected patients and control individuals. Exon 15 of the CETP gene was amplified through PCR and was subjected to Sanger sequencing. Multiple sequence alignment was carried out to screen mutations. Sequence analysis revealed no mutation at exon 15 of the CETP gene. Thus, mutation of CETP in exon 15 did not provide any significant information for the prediction of CVD in the Bangladeshi population.


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