Evaluation of bio-wastes for multiplication of paecilomyces lilacinus

Paper Details

Research Paper 01/06/2017
Views (218) Download (11)

Evaluation of bio-wastes for multiplication of paecilomyces lilacinus

Z. M. Mwathi, W. M. Muiru, J. W. Kimenju, P. Wachira
Int. J. Agron. Agri. Res.10( 6), 1-5, June 2017.
Certificate: IJAAR 2017 [Generate Certificate]


Three agricultural based solid carriers namely barley, rice bran and rice husks were used in this experiment for continuous production of Paecilomyces lilacinus. The objective of this experiment was to establish the carrier that supported the highest growth of the fungi. A completely randomized design replicated four times was conducted in agriculture laboratory at Karatina University. The variable considered in this experiment was the colony forming units in each carrier at day 7, 14, 21 and 28 days after inoculation. Data collected was analyzed using Analysis of variance (ANOVA) with portable Genstat package. Separation of Means was done using Duncan’s Multiple range test at p<0.05.In this experiment, barley exhibited the highest spore count of 3.38 x 108 on the 28th day of the experiment. Barley was followed closely by rice bran with a spore count of 2.99×108. With time there was an increment in the number of spores from week one of inoculation to the fourth week. Growth of this fungus was significant in barley carrier. This led to the conclusion that barley can be considered as a suitable carrier that can be used for continuous production of Paecilomyces spp. fungi for effective management of nematodes in bananas.


Amala U, Jiji T, Naseema A.  2012. Mass multiplication of entomopathogenic fungus (Paecilomyces lilacinus) (Thom) Samson with solid substrates: Jbiopest 5(2), 168-170. www.jbiopest.com/users/lw8/efiles/vol_5_2_168-170-12067.pdf

Gerhardson B.  2002. Biological substitutes for pesticides. Trends  Biotechnology. 20, 338-343.

Gogoi BB, Neog PP. 2003.  Vegetable and pulse crops –host for culturing bacteria antagonists of root-knot nematodes. Annals of Plant Protection Sciences, 11, 403-404.

Gortari MC, Galarza BC, Cazau MC, Hours RA.  2008.   Comparison of the biological properties of two strains of Paecilomyces lilacinus (Thom) Samson associated to their antagonistic effect onto Toxocara canis eggs. Malaysian Journal of Microbiology, 4(2), 35- 41.

Humber RA.   2008. Evolution of entomopathogenicity in fungi. Journal of Invertebrate  Pathology 98(3), 262-266. http://dx.doi.org/10.1016/j,jip.2008.02.

Hutchinson  CM, Mcgiffen ME, Sims JJ, Bechkerj O.  1999.  Efficacy of methyl iodide soil fumingation for control of Meloidogyne incognita, Tylenchulus semipenetrans and Heterodera schashtii. Nematology 1, 407-414.

Jagadeesh BCS, Venkatachalapathy CM, Anitha CN. 2008.  Evaluation of locally available substrates for mass multiplication of entomopathogenic fungi Metarhizium anisopliae (Metch.) Sorokin. Journal of Biopesticides 1(2), 146-147.

Gulsar JB, Rohini I, Gunasekaran M.  2006.  Mass multiplication and formulation of a nematophagous fungus, Paecilomyces lilacinus. International Journal of Nematology 16(2), 145-152.

Kerry B,  Hidalgo-Diaz L. 2004.   Application of Pochonia chlamydosporia in the integrated control of root-knot nematodes on organically grown vegetable crops in Cuba. In: Sikora, R.A., Gowen, S., Hauschild, R., Kiewnick, S. (Eds.), Mcittitrophic infractions in soil and integrated control. IOBC/ WPRS Bulletin. 27, 123-126.

Khan A, Williams K, Nevalainen H.  2003.  Testing the nematophagus biological control strain Paecilomyces lilacinus 251 for paecilotoxin production. Fems Microbiology Letters 227, 107 –111. http://onlinelibrary.wiley.com/doi/10.1016/S03781097(03)00654-2/pdf.

Kiewnick S, Sikora RA.  2006. Biological control of the root -knot nematode Meloidogyne incognita by Paecilomyces lilacinus Strain 251. Biological Control 38, 179-187.

Mucksood AG, Tabreiz AK. 2013. In vitro studies of bio-wastes on growth and sporulation of fungal bio-agents. African Journal of Agricultural research 8(37), 4660-4663. http://dx.doi.org/10.5897/AJAR 10.585

Mucksood AG, Tabreiz AK.  2010. Biological potential of Paecilomyces lilacinus on pathogenesis of Meloidogyne javanica infecting tomato plant. European Journal of Applied Sciences 2(2), 80-84.

Nico AI, Jimenez-Diaz RM, Castillo P. 2004. Control of root-knot nematodes composted agro-industrial wastes in potting mixtures. Crop Protection. 23, 581-587.

Pandey  A, Soccol CR,   Poonam N, Soccol VT, Vandenberghe LPS.   2000. Biotechnological potential of agro-industrial residues ii: cassava bagasse. Bio-resource Technology 74, 81-87.

Shanthi A, Rajendran G.  2006. Biological control of lesion nematodes in bananas. Nematologia Mediterranea 34, 69-75.

Sharon E, Bar-Eyal M, Chet I, Herrera-Estrella A, Kleifeld O, Spiegel Y.  2001. Biological control of the root-knot nematode Meloidogyne javanica by Trichoderma harzianum. Phytopathology 91, 687-693.

Sikora RA, Fernande ZE. 2005. Nematode parasites of vegetables. In: Luc, M., Sikora , R.A., Bridge, J. (Eds.), Plant–parasitic nematodes in subtropical and tropical agriculture, Second Ed. Cabi Publishing,Wallingford,Uk.319-392. http://dx.doi.org/10.1079/9780851997278.0319.

Soccol CR, Vandenberghe LPS. 2003. Overview of applied solid state fermentation in Brazil. Biochemical Engineering Journal. 3, 205-218. http://dx.doi.org/10.1016/S1369-703X(02)001333-X

Somaseka N, Mehta UK, Hari K.  1998. Evaluation of sugarcane by-products for mass multiplication of nematode antagonistic fungi In: Nematology challeges and opportunities in 21st century. Coimbatore India: Proceedings of the Third International Symposium of Afro-Asian Society of Nematologists 199-202.

Sossamma VK, Jayashree. 1999.  Evaluation of some plant leaves for mass multiplication of the nematophagous fungus, Paecilomyces lilacinus. Indian Journal of Nematology 29, 103-104.

Thet TM, Saisamorn L.  2012. Conidial production of entomopathogenic fungi in solid state fermentation. KKU Research Journal, 17(5), 762-768. http://resjournal.kku.ac.th

Verma AC, Singh HK, Khan MN.  2004.  Evaluation of substrates for mass multiplication of Paecilomyces lilacinus. Annals of Plant Protection Sciences 12, 459-460.