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Evaluation of CHROMagar Candida in the rapid identification of medically important species of Candida

Research Paper | July 1, 2017

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Lucilyn D. Lahoylahoy, Bernadette C. Mendoza

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Int. J. Biosci.11( 1), 380-385, July 2017

DOI: http://dx.doi.org/10.12692/ijb/11.1.380-385


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Providing a quick and effective method of identification of the important human pathogen Candida species is a vital step in delivering prompt and efficient disease treatment and determining antifungal susceptibility profile. This study was conducted with the aim of evaluating the use of CHROMagar Candida in the rapid presumptive taxonomical identification of C. albicans, C. parapsilosis, and C. krusei. The previous taxonomic classification of 188 Candida species were reconfirmed through the standard traditional methods of yeast purification, morphological and colonial characterization, and standard biochemical tests of sugar assimilation and fermentation. The traditional methods reconfirmed that all isolates belonged to the genus Candida: C. albicans (126), C. parapsilosis (41), and C. krusei (21). CHROMagar Candida (CA), a selective medium claimed to differentiate Candida species based on colony color, was compared to the germ tube test (GTT) in the presumptive identification of C. albicans.  The use of GTT only confirmed 100 out of the 126 (79%) C. albicans isolates as compared to the 116 (92%) C. albicans verified using the CA medium. The use of CA gave a sensitivity of 92.1%, specificity of 98.4%, and accuracy of 94.1% compared with the 79.4% sensitivity, 70.5% specificity, and accuracy of 86.2% obtained for GTT. Overall, the better method to use for rapid and presumptive identification of C. albicans and C. parapsilosis is CHROMagar Candida.


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Evaluation of CHROMagar Candida in the rapid identification of medically important species of Candida

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