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Evaluation of drought tolerance in mutant Kenyan bread wheat (Triticum aestivum L.) using in vitro techniques

Research Paper | August 1, 2016

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Gerald Gikonyo Githinji, Miriam Kinyua, Oliver Kiplagat, Rael Birithia

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Int. J. Agron. Agri. Res.9( 2), 180-189, August 2016


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Wheat (Triticum aestivum L) is widely cultivated as a small-grain cereal. In Kenya, it is ranked second after maize in its contribution towards food security. Biotic stress conditions such as drought cause extensive losses to agricultural production worldwide. In Kenya, arid and semiarid lands represent 83% of total land area, which experience frequent crop failure due to drought stress. Developing drought-tolerant wheat genotypes has been the focus of many wheat improvement programs. Few drought tolerant varieties are available for commercial production in Kenya. Hence, there is need to develop more drought tolerant wheat varieties. The objective of this study was to screen for drought resistance in two mutant wheat lines in vitro using Polyethylene Glycol (PEG). Four wheat germplasm were tested for drought tolerance using -3.0, -9.0 and -15.0 PEG-6000 concentrations and the data was recorded on various seedling parameters including root length, shoot length and root length /shoot length ratio. The experiment was carried out in three replicates using completely randomized design. Data was subjected to analysis of variance (ANOVA) using GENSTAT 12th edition. Correlation was done by Pearson Correlation Coefficients to determine significant associations among the different variables. Results indicated that there was a significant difference (p=0.05) between Mutant 1 and Mutant 2 having longer roots, shoots and a root to shoot ratio compared to Chozi and Duma in the different PEG concentrations used. Hence, the two mutant lines are possible candidates for varieties that can be grown in ASALs regions in Kenya.


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Evaluation of drought tolerance in mutant Kenyan bread wheat (Triticum aestivum L.) using in vitro techniques

Abdel-Raheem AT, Ragab AR, Kasem ZA, Omar FD, Samera AM. 2007. In vitro selection for tomato plants for drought tolerance via callus culture under polyethylene glycol (PEG) and mannitol treatments. African Crop Science Society 8, 2027-2032.

Abdi AA, Badawy SA, Zayed BA, ElGohary AA. 2010. The role of root system traits in the drought tolerance of rice (Oryza sativa L.). World Academy of Science, Engineering and Technology 68, 1378–1382.

Abedi T, Pakniyat H. 2010. Antioxidant enzyme changes in response to drought stress in ten cultivars of oilseed rape (Brassica napus L.). Czech Journal of Genetics and Plant Breeding 46, 27–34.

Acquaah G. 2012. Principles of Plant Genetics and Breeding, 2nd Edition. Wiley-Blackwell. USA.

Al-Ghamdi AA. 2009. Evaluation of oxidative stress tolerance in two wheat (Triticum aestivum) cultivars in response to drought. International Journal of Agriculture and Biology 11, 7–12.

Ambika R, Rajendran A, Muthiah R, Manickam A, Shanmugasundaram P, Joel AJ. 2011. Indices of drought tolerance in Sorghum (Sorghum bicolor L. Moench) genotypes at early stages of plant growth. Research Journal of Agriculture and Biological Sciences 7, 42-46.

Anand A, Trick HN, Gil BS. 2003. Stable transgene expression and random gene silencing in wheat. Plant Biotechnology Journal 14, 241–251.

Bayoumi TY, Eid MH, Metwali EM. 2008. Application of physiological and biochemical indices as a screening technique for drought tolerance in wheat genotypes. The African Journal of Biotechnology 7, 2341-2352.

Bibi A, Sadaqat HA, Tahir MHN, Akram HM. 2012. Screening of sorghum (Sorghum bicolor var Moench) for drought tolerance at seedling stage in polyethylene glycol. The Journal of Animal and Plant Sciences 22(3), 671-678.

Dhanda SS, Sethi GS, Behl RK. 2004. Indices of drought tolerance in wheat genotypes at early stages of plant growth. Journal of Agronomy and Crop Science 19, 6–8.

Frazer TE, Silk WK, Rost TL. 1990. Effect of low water potential on cortical cell length in growing region of maize roots. Plant Physiology 93, 648–651.

Geleta N, Negasa D, Teshome D. 2015. Evaluation of bread wheat (Triticum aestivum L.) breeding lines for yield and yield related characters in Horo Guduru Wollega Zone, Western Ethiopia. Science, Technology and Arts Research Journal 4(1), 1- 8.

Geravandi M, Farshadfar EM, Kahrizia D. 2011. Evaluation of some physiological traits as indicators of drought tolerance in bread wheat genotype.Russian journal of Plant Physiolology 58(1), 69-75.

Gesimba RM. 2000. Selection of drought resistant bread wheat through shoot and root characteristics at seedling stage. M.Sc. Thesis, Egerton University Graduate School pp. 1-100.

Govindaraj M, Shanmugasundaram P, Sumathi P, Muthiah AR. 2010. Simple, rapid and cost effective screening method for drought resistant breeding in pearl millet Electron. Journal of Plant Breeding 1, 590–599.

Goyal A, Manoharachary C. 2014. Future Challenges in Crop Protection Against Fungal Pathogens. Springer Science. New York.

Hadas A. 1976. Water uptake and germination of leguminous seeds under changing external water potential in osmotic solutions. Journal of Experimental Botany 27(3), 480-489.

Hamayun M, Khan SA, Shinwari ZK, Khan AL, Ahmad N, Lee IJ. 2010. Effect of polyethylene glycol induced drought stress on physio-hormonal attributes of soybean. Pakistan Journal Botany 42, 977-986.

Hartmann M, College P, Lumsden. 2005. Responses of different varieties of Loliumperenne to salinity. Annual Conference of the Society for Experimental Biology, Lancashire.

International Atomic Energy Agency. 1998. Application of Biotechnology and Mutation Techniques for the Improvement of Local Food Crops in LIFDCs. Report of First RCM, Vienna, Austria.

Kamran M, Shahbaz M, Ashraf M, Akram NA. 2009. Alleviation of drought-induced adverse effects in spring wheat (Triticum aestivum L.) using proline as a pre-sowing seed treatment. Pakistan Journal of Botany 41(2), 621-632.

Kaur S, Gupta AK, Kaur N. 1998. Gibberellic acid and kinetin partially reverse the effect of water stress on germination and seedling growth in chickpea. Plant Growth Regulators 25, 29–33.

Khodarahmpour Z. 2011. Effect of drought stress induced by polyethylene glycol (PEG) on germination indices in corn (Zea mays L.) hybrids. African Journal of Biotechnology 10(79), 18222-18227.

Kinyua MG, Wanga H, Malusznyski M, Wanjama JK, Wambanyi O. 1998. Application of mutation techniques in the development of drought tolerant wheat varieties in Kenya. In: Proceedings of the 6th Biennial KARI Scientific Conference. Kenya Agricultural Research Institute, Nairobi, Kenya p. 226-233.

Kinyua MG. 1997. Transfer of genes of resistance to yellow rust (Puccinia triifarmis L.) from wild emmer Triticum dicoccoides into commercial varieties. University of Nairobi, Kenya.

Kosturkova G, Todorova R, Dimitrovai M, Tasheva K. 2014. Establishment of Test for Facilitating Screening of Drought Tolerance in Soybean. Series F. Biotechnologies, Vol. XVIII.

Kulkarni M, Deshpande U. 2007. In Vitro screening of tomato genotypes for drought resistance using polyethylene glycol. African Journal of Biotechnology 6, 691-696.

Mafakheri A, Siosemardeh A, Bahramnejad B, Struik PC, Sohrabi Y. 2010. Effect of drought stress on yield, proline and chlorophyll contents in three chickpea cultivars. Australian Journal of Crop Science 4(8), 580-585.

Matsuura A, Inanaga S, Sugimoto Y. 1996. Mechanism of interspecific differences among four graminaceous crops in growth response to soil drying. Japanese Journal of Crop Science 65, 352-360.

Meeta J, Mini M, Rekha G. 2013. Effect of PEG-6000 Imposed Water Deficit on Chlorophyll Metabolism in Maize Leaves. Journal of Stress Physiology and Biochemistry Vol. 9 No. 3, p. 262-271.

Ndiema AC, Aboud AA, Kinyua MG, Keya CON. 2011. Farmer perception in adoption of drought tolerant wheat in arid and semi-arid region of Kenya. African Crop Science Conference Proceedings Vol. 10. p. 359 – 363.

Ndiema AC. 2010. Factors influencing adoption of drought tolerant wheat varieties in the arid and semi-arid lands of Narok and Kajiado Districts Rift Valley Province of Kenya. Unpublished Degree of Doctor of Philosophy in Agricultural Extension of Egerton University thesis.

Prasad PVV, Staggenborg SA, Ristic Z. 2008. Impact of drought and heat stress on physiological, growth and yield processes. In L. H. Ahuja and S. A. Saseendran (Eds.) Modeling water stress effects on plant Growth Processes, Vol. 1 of the Advances in Agricultural Systems Modeling: Trasndisciplinary Research, Synthesis and Application Series. Madison, Wisconsin: ASA-CSSA.

Radhouane L. 2007. Response of Tunisian autochthonous pearl millet (Pennisetum glaucum L.) to drought stress induced by polyethylene glycol (PEG) 6000. African Journal of Biotechnology 6, 1102-1105.

Raziuddin Z, Swati J, Bakht B, Ullah M, Shafi M, Akmal M, Hassan G. 2010. In situ assessment of morpho-physiological response of wheat (Triticum aestivum L.) genotypes to drought. Pakistan Journal of Botany 42(5), 3183-3195.

Ridge I. 1991. Plant Physiology, Hodder and Stoughton. The Open University, Milton Keynes.

Sayar R, Khamira H, Kameli A, Mosbahi M. 2011. Physiological tests as predictive appreciation of drought tolerance in durum wheat (Triticum durum Desf) Agronomic Research 6(1), 79–90.

Singh R, Huertu-Espino J, Sharma R. 2007. High yielding spring wheat germplasm fro global irrigated and rain fed production systems Euphytica 157, 351–363.

Takele A. 2000. Seedling emergence and of growth of sorghum genotypes under variable soil moisture deficit. Acta Agronomica Hungarica 48(1), 95-102.

Torkamani J. 2005. Using a whole-farm modelling approach to assess prospects of technologies under uncertainty. Agricultural systems 85(2), 138-154.

United States Department of Agriculture (USDA). 2011: Economic Research Services Food Consumption Database. In http://www.ers.usda.gov/data/foodconsumption/, USDA-ERS: 2011.

United States Department of Agriculture (USDA). 2013. Developing Countries Dominate World Demand for Agricultural Products. USDA, Economic Research Service. www.ers.usda.gov/amber-waves/2013- august/developing-countries-dominate-world-demand-for-agricultural-products.aspx#.Vx4zZNJ94dV.