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Evaluation of genetic diversity in durum wheat genotypes (Triticum turgidum var. durum) using ISSR markers

Maryam Razmjoo, Reza Mohammadi, Lia Shooshtari

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J. Bio. Env. Sci.6(1), 522-529, January 2015

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Abstract

This study was investigated to evaluate the genetic diversity among 25 genotypes of durum wheat (23 breeding lines, two local varieties and one improved cultivar) at the biotechnology laboratory of Islamic Azad University, Kermanshah, Iran in 2010-2011. 10 inter-simple sequence repeats (ISSR) markers were used to investigate genetic diversity among tested genotypes. A total of 70 loci were scored by 10 primers. The average polymorphic information content (PIC) was 0.41, and the highest amount PIC was 0.49 related to primer IS12. The highest genetic distance between genotypes based on Dice similarity coefficients was observed between genotypes 23 and 24 and the least genetic distance was between genotypes 13 and 18. Based on Dice similarity coefficients, genetic distance of genotypes varied from 0.38 to 0.90 with mean value equal to 0.64. Cluster analysis based on Ward’s method and principal coordinate analysis (PCoA) classified the genotypes into five main groups. The results revealed that ISSR markers could be efficiently used to evaluate genetic variation in the durum wheat genotypeswheat germplasm.

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Evaluation of genetic diversity in durum wheat genotypes (Triticum turgidum var. durum) using ISSR markers

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