Evaluation of genetic diversity in weed dodder (Cuscuta epithymum L) on Northern of Iran by ISSR molecular marker
Paper Details
Evaluation of genetic diversity in weed dodder (Cuscuta epithymum L) on Northern of Iran by ISSR molecular marker
Abstract
Weed dodder (Cuscuta epithymum L) is a parasitic vine that attacks the aerial parts of many shrubs, trees, and is used widely in medicine. In this study for first time in world, Inter Simple Sequence Repeat (ISSR) technique was employed for determine genetic diversity of 20 genotype of weed dodder from Golestan and Mazandaran provinces. Six oligo nucleotide primers were used to amplify the genomic DNA isolated from the dried stems. 6 ISSR primers amplified 90 bands that 76.33% bands were polymorphism. Average polymorphism information content (PIC) and average marker index (MI) value was 0.26 and 20 for ISSR markers respectively. Cluster analysis based on DICE coefficient and UPGMA algorithm showed that there is wide variation in samples collected. The average of similarity based on ISSR markers was 0.57. This high level of diversity could be related to the existents of several sub species in weed dodder (Cuscuta epithymum L.) and the high discrimination power of molecular markers that be used in this study. ISSR molecular marker may serve as a complementary tool for determine of genetic diversity.
Doyle J. 1991. DNA protocols for plants – CTAB total DNA isolation. In: Hewitt GM, Johnston A (Eds.) Molecular Techniques in Taxonomy. Springer, Berlin.
Doyle JJ, Doyle JL. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemistry Bulletin 19, 11-15.
Fasih Z, Farshafvar M, Safari H. 2013. Genetic diversity evaluation of within and between population for Festuca arundinacea by ISSR markers. International Journal of Agriculture and Crop Sciences 5(13), 1468-1472.
Gustine DL, Voigt PW, Brummer E, Papadopuolos A. 2002. Genetic variation of RAPD markers for North American white clover collections and cultivars. Crop Science 42, 343- 347.
Khan K, Prasad MN. 2010. Optimization of DNA isolation and PCR protocol for RAPD analysis of selected medicinal and aromatic plants of conservation concern from Peninsular India. African Journal of Biotechnology 5 (3), 230-234.
Mahmood MK, Luikart G, Waples RS. 1997. Genetic monitoring as a promising tool for conservation and management. Trends Ecol Evol 22, 25–33.
Mclelland DL, Voigt PW, Brummer E, Papadopuolos A. 1995. Genetic variation of RAPD markers for North American white clover collections and cultivars. Crop Science 42, 343- 347.
Me J, Heydarinezhad AM, Niridehghan M. 1999. The specialist Hemiptera associated with Mistletoe. British Journal of Entomology and Natural History 12, 237-238.
Mejnartowicz L.2004. Genetic Analysis of Silver-fir population in the North Carpathian and Sudeten Mountains. Acta Soc. Bot. Pol 65 (3-4), 319-328.
Nei M. 1972. Genetic distance between populations. Am. Nature 106, 283-292.
Paeker H. 1993. European dodder – taxonomy, host trees, parts used, physiology. in. Bussing. Amsterdam: Harwood Academic Publishers 16, 120-235.
Palet, R. and Smouse PE. 2006. GenAlex 6: Genetic analysis in excel. population genetic software for Teaching and research. Molecular Ecology Notes 6, 288-295.
Parker, H. and Riches, K. 2008. Botany of European dodder (Cuscuta sp). Oncology 43, 2–7.
Paudit, H. 1386. Iranian culinary culture. 1th. Publication of Agricultural Sciences. 4, 103-102.
Peakle, A. Smouse, M. 2006. Apoptosis -inducing properties of Cuscuta epithymum L., correlate with their content of toxic dodder. Biotech Research 9, 203-208.
Sadeghi AA, Fakheri B, Kazemitabar SK, Gholinezhad R. 2012. Optimization of thermo cycler set to determine of genetic diversity on weed dodder. Chalous journal of azad Islamic university of iran. 23(7).
Sandler J, Sadeghi A, Farster J. 2001. Weed dodder – distribution, biology and the national survey. British Wildlife 7(2), 25-28.
Uddin H, Ghoiyd K, Berahmani S. 2007. Dodder lectins and their A and B chains. Oncology 43, 23-34.
Amin Sadeghi Alikelayeh, Seyed Kamal Kazemitabar, Baratali Fakheri, Leila Fahmideh, 2014. Evaluation of genetic diversity in weed dodder (Cuscuta epithymum L) on Northern of Iran by ISSR molecular marker. J. Biodiv. Environ. Sci., 4(1), 221-227.
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