Paper Details

Evaluation of genetic diversity of wild-type barley (Hordeum vulgare L. sub sp. Spontaneum) based on storage proteins polymorphism

Monireh Rahimi, Islam Majidi Harvan, Mostafa Valizadeh, Farokh Darvish Kajori, Farshad Ebrahimpour

DOI: http://dx.doi.org/10.12692/ijb/5.4.108-116

Int. J. Biosci. 5(4), 108-116. August, 2014. (PDF)

Abstract:

Today, most scientists have accepted that cultivated barley was originated from wild-type barley, Hordeum spontaneum. By the way, this is unclear whether domestication processes just occurred in Fertile Crescent or simultaneously in different areas all over the world. Additionally, there are disagreements in relation to center or main diversity centers of this plant. In the present study, in order to evaluate genetic and geographic diversity and also determining of barley populations phylogenic relations, 266 wild-type, H. spantaneum, from areas located in or out of Fertile Crescent and also 44 samples of cultivated barley from different world areas were studied. Barley samples storage proteins were analyzed by SDS-PAGE method and were assessed in three hordein groups including D, C and B. Genetic parameters, such as patterns number and genetic diversity index showed that by getting away from Fertile Crescent, barley genetic diversity was reduced, However, North Africa and East Asia could be considered as a secondary centers of diversity of the plant. The study of genetic distances showed that wild barley genotypes of Iraq and Turkey could be the nearest populations to modern cultivated barley samples. Based on these results, Fertile Crescent especially Iran, Iraq, South East of Turkey and Jordan could be main centers of diversity of wild barley H. Spontaneum.