Evaluation of genotoxic and antigenotoxic activities of resveratrol in the wing spot test of Drosophila

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Research Paper 01/08/2015
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Evaluation of genotoxic and antigenotoxic activities of resveratrol in the wing spot test of Drosophila

Eylem Eroğlu Doğan, Elif Yeşilada
Int. J. Biosci.7( 2), 86-95, August 2015.
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Resveratrol (RES) is a well-known polyphenolic compound found in plants and has received much interest due to its numerous biological activities. In this study, the potential genotoxic activity of resveratrol (RES) and its antigenotoxic properties against the mutagenic agents cyclophosphamide (CP), mitomycin C (MMC) and N-methyl-N-nitrosourea (MNU) were investigated using the standard (ST) cross of the wing spot test in Drosophila melanogaster. It was shown that five different concentrations of RES (0.1, 0.2, 0.5, 0.75, 1 mM) employed had no significant effect on spots frequencies indicating a lack of genotoxic activity; while CP (0.5, 1, 2 mM), MMC (0.025, 0.05, 0.1 mM) and MNU (0.5, 1, 1.5 mM) treatments gave positive results for all types of spots, indicating a strong genotoxic activity. Τhe simultaneous administration of CP (1 mM), MMC (0.05 mM) and MNU (1 mM) with RES (0.2, 0.5, 1 mM) led to considerable alterations of the frequencies of CP, MMC and MNU-induced wing spots with the total mutant clones showing reduction between 16.25% and 55.25%. The data clearly indicate a protective role of RES against CP, MMC, MNU-induced genotoxicity.


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