Evaluation of hepato-protective activity of B. lycium methenolic crude extracts collected from Distric Sherani, Balochistan

Paper Details

Research Paper 01/06/2019
Views (300) Download (15)

Evaluation of hepato-protective activity of B. lycium methenolic crude extracts collected from Distric Sherani, Balochistan

Sami Ullah Sherani, Javied Iqbal, Amanullah Khan, Muhammad Ali Khan, Nadeem Rashid, Mohammad Rahim Niazi, Zia Ud Din, Mohamad Kamran Taj
J. Bio. Env. Sci.14( 6), 77-83, June 2019.
Certificate: JBES 2019 [Generate Certificate]


Berberis lycium, which is commonly called as Indian barberry (English) and Kashmal or Ishkeen in Urdu, is a spiky plant which is the member of the genus Berberis of family Berberidaceae. B. lycium also includes anti-hepatotoxicity effect, when was mixed with G. aparine and P. integerrima and was tested in rats that were treated with carbon tetra chloride; the results revealed that the combination of these three medicinal plants encompasses anti hepatotoxicity effects. B. lycium (root) sample was collected from the hill of village of Ahmadedergah near to Tahkhta Suleiman District Sherani Balochistan, Pakistan. The collected samples were identified by Pharmacognosy, Department faculty of Pharmacy University of Baluchistan, Quetta. Adult healthy rabbits (male) having weights approximately 950g-1300g, were kept in animals house at CASVAB, UoB. Sample serum, within 3 hours after collection , was analyzed  for certain biochemical parameters (Alkaline phosphatase (ALP), Alanine aminotransferase (ALT), Gamma glutamyl transpeptidase (γ-GT), Aspartate aminotransferase (AST), and total Proteins like Bilirubin,  Albumin and Globulin) through automatic analyzer (Merck) at 37oC through standard reagent kits. The obtained values for LFT in the current portion of controlled group were, 0.76± 0.060, 0.032±0.008(mg/dL), 4.2±0.802, 93.8±1.500, 2.28±0.107 and 273±2.818(U/L) for TB, DB, ALT, AKP, GGT, SGOT and SGPT (AST) levels. Whereas, the group treated with CCl4 these parameters were, 0.86±0.075, 0.064+0.006(mg/dL), 4.6±0.680, 2.44±0.150, 90.4±3.467 and 218.4±1.439(U/L) level. While, the group treated with CCl4 and B. lycium 500mg, the above parameters were calculated as 0.92±0.086, 0.442±0.228(mg/dL), 4.4±0.601, 93.2±1.244, 2.62±0.097 and 221.6±1.540(U/L), respectively.


Abere TA, Okoto PE, Agoreyo FO. 2010. Antidiarrhoea and toxicological evaluation of the leaf extract of Dissotis Rotundifolia triana (Melastomataceae). BMC complementary and alternative medicine 10(1), 71.

Abere TA, Okoto PE, Agoreyo FO. 2010. Antidiarrhoea and toxicological evaluation of the leaf extract of Dissotis Rotundifolia triana (Melastomataceae). BMC complementary and alternative medicine 10(1), 71.

Agyare C, Asase A, Lechtenberg M, Niehues M, Deters A, Hensel A. 2009. An ethnopharmacological survey and in vitro confirmation of ethnopharmacological use of medicinal plants used for wound healing in Bosomtwi-Atwima-Kwanwoma area, Ghana. Journal of Ethnopharmacology 125(3), 393-403.

Ahmad M, Alamgeer Sharif T. 2009. “A potential adjunct to Insulin: Berberis lycium Royle.” Diabetologia Croatica 38(1), 13-18.

Ahmad M. 2008. “Hepatoprotective effect of Berberis lycium (Royle) in hepatotoxic rabbits”. Gomal University journal of research 24, 24.

Amin A, Hamza AA. 2005. Oxidative stress mediates drug-induced hepatotoxicity in rats: A possible role of DNA fragmentation. Toxicology 208, 367-375.

Asif A. 2007. “Wound healing activity of root extracts of Berberis lycium Royle in rats”. Phytotherapy Research 21(6), 589-591.

Beers SJ. 2012. Jamu: the ancient Indonesian art of herbal healing. Tuttle Publishing.

Chauhan NS. 1999. Medicinal and aromatic plants of Himachal Pradesh. Indus publishing

Cowan MM. 1999. “Plant products as antimicrobial agents”. Clinical microbiology reviews 12(4), 564-582.

Demori I, Voci A, Fugassa E, Burlando B. 2006. Combined effects of high-fat diet and ethanol Induce oxidative stress in rat liver. Alcohol 40, 185-191.

Dhar S. 2012. “In vitro plant regeneration system for Berberis lycium using cotyledonary node explants”. Journal of Tropical Medicinal Plants  13(1), 51-55.

Eisenberg DM, Davis RB, Ettner SL, Appel S, Wilkey S, Van Rompay M, Kessler RC. 1998. Trends in alternative medicine use in the United States, 1990-1997: results of a follow-up national survey. Jama 280(18), 1569-1575.

Eisenberg DM, Kessler RC, Foster C, Norlock FE, Calkins DR, Delbanco TL. 1993. Unconventional medicine in the United States–prevalence, costs, and patterns of use. New England Journal of Medicine 328(4), 246-252.

Imtiaz UH, Manzoor H. 2003. “Medicinal plants of Mansehra”. Hamdard medicus 36, 69.

Ivanovska N, Philipov S. 1996. “Study on the anti-inflammatory action of Berberis vulgaris root extract, alkaloid fractions and pure alkaloids”. International journal of Immunopharmacology 18(10), 553-561.

Jabeen N, Saleem A, Anwaar S, Hussain Z. 2015. Berberis lycium Royle (Royle, 1837): A threatened medicinal plant and Its biological activities. EC Agri-culture 1, 100-108.

Jafri SMH. “Berberidaceae”. Flora of Pakistan.

Joseph B, Jini D. 2011. “Insight into the hypoglycaemic effect of traditional Indian herbs used in the treatment of diabetes”. Research Journal of Medicinal Plant 5(4), 352-376.

Kim YJ, Park T. 2008. Genes are differentially expressed in the epididymal fat of rats rendered obese by a high-fat diet. Nutr. Res 28, 414-422.

Lee CH, Olson P, Evans RM. 2003. Minireview: lipid metabolism, metabolic diseases, and peroxisome proliferator-activated receptors. Endocrinology 144(6), 2201-2207.

Malik AR, Siddique MAA, Sofi PA, Butola JS. 2011. Ethnomedicinal practices and conservation status of medicinal plants of North Kashmir Himalayas. Res. J. Med. Plant 5, 515-530. Med. Coll. 2003, 25, 244-246.

Myagmar BE, Shinno E, Ichiba T, Aniya Y. Antioxidant activity of medicinal herb Plants and human health in the twenty-first century. TRENDS in Biotechnology 20(12), 522-531.

Okamura H. 1993. “Antioxidant activity of tannins and flavonoids in Eucalyptus rostrata”. Phytochemistry 33(3), 557-561.

Paul D. 2010. Studies on the Medicinal Flora of Amritsar District.

Potdar D. 2012. “Phyto-chemical and pharmacological applications of Berberis aristata”. Fitoterapia 83(5), 817-830.

Raskin I, Ribnicky DM, Komarnytsky S, Ilic N, Poulev A, Borisjuk N, … O’Neal JM. 2002. Rhodococcum vitisidaea on galactosamine-induced liver injury in rats. Phytomedicine 2004, 11.

Rong XL, Kim MS, Su N, Wen SP, Matsuo Y, Yamahara J, Murray M, Li YH. 2008. An aqueous extract of Salacia oblonga root, a herb-derived peroxisome proliferator-activated receptor-alpha activator, by oral gavage over 28 days induces gender-dependent hepatic hypertrophy in rats. Food Chem. Toxicol 46, 2165-2172.

Royle JF. 1834. “On the Lycium of Dioscorides”. Transactions of the Linnean Society of London 17(1), 83-94.

Sabir S. 2013. “Phytochemical and antioxidant studies of Berberis lycium.” Pakistan journal of pharmaceutical sciences 26(6), 1165-1172.

Shamsa F. 1999. “Antithistaminic and anticholinergic activity of Berbery fruit (Berberis vulgaris) in the guinea pig ileum”. Journal of Ethnopharmacology 64(2), 161-166.

Thounaojam MC, Jadeja RN, Valodkar M, Nagar PS, Devkar RV, Thakore S. 2011. Oxidative stress induced apoptosis of human lung carcinoma (A549) cells by a novel copper nanorod formulation. Food and chemical toxicology 49(11), 2990-2996.

Yang Z, Zhai W. 2010. “Identification and antioxidant activity of anthocyanins extracted from the seed and cob of purple corn (Zea mays L.)”. Innovative Food Science & Emerging Technologies 11(1), 169-176.

Yang JJ, Li GL, Liu HR, Ren BB. Effect of eveningrose oil on activities of oxygen free radical scaverging-related enzymes and hepatic morphosis in rats on high lipid diet. J. NingXia

Yang JS, Lee SJ, Park HW, Cha YS. 2006. Effect of genistein with carnitine administration on lipid parameters and obesity in C57Bl/6J mice fed a high-fat diet. J. Med. Food 9, 459-467.

Zovko K. 2010. “Evaluation of antioxidant activities and phenolic content of Berberis vulgaris L. and Berberis croatica Horvat”. Food and chemical toxicology 48(8-9), 2176-2180.