Evaluation of in-vitro antibacterial activity of leaf extracts of three species of family Oleaceae

Paper Details

Research Paper 01/07/2016
Views (307) Download (13)

Evaluation of in-vitro antibacterial activity of leaf extracts of three species of family Oleaceae

Rizwana Rafique, Zaheer-Ud-Din Khan, Sundas Altaf, Anjum Parveen
J. Bio. Env. Sci.9( 1), 150-158, July 2016.
Certificate: JBES 2016 [Generate Certificate]


The present study was carried out to determine the antibacterial potential of the extracts of Jasminum sambac (L.) Aiton, Olea europaea L. and O. ferruginea Royle leaf against Staphylococus aureus and Bacillus subtilis (Gram positive bacteria) and. Escherichia coli and Pseudomonas aeruginosa (Gram negative bacteria) by agar well diffusion method. The extracts were obtained by maceration in solvents, such as n-hexane, chloroform, ethanol and distilled water. The zone of inhibition of the leaf extracts recorded as antibacterial potential was compared with that of the different standard antibiotics, like amikacin (30µg), erythromycin (10µg) etc. The n-hexane extracts of Jasminum sambac showed highest activity (26.37±2.54 mm) against E. coli. Aqueous and ethanol extracts of all the plants exhibited comparatively higher antibacterial potential against Gram negative bacteria than the Gram positive bacteria. The Minimum Inhibitory Concentration (MIC) was also determined for all the extracts. The results supported the ethnomedicinal use of Jasminum sambac (L.) Aiton, Olea europaea L. and O. ferruginea Royle to treat various bacterial infections in man.


Adnan M, Bibi R, Mussarat S, Tariq A, Shinwari ZK. 2014. Ethnomedicinal and phytochemical review of Pakistani medicinal plants used as antibacterial agents against Escherichia coli. AnnalsofClinicalMicrobiologyand Antimicrobials 13, 40.

Akinyemi KO, Oluwa OK, Omimigbehin EO. 2006. Antimicrobial activity of crude extracts of three medicinal plants used in south- west Nigerian folk medicine on some food borne bacterial pathogens. African Journal of Traditional, Complementary and Alternative Medicine 3, 13-22.

Auwal MS, Mairiga IA, Shuaibu A, Ibrahim A, Gulani IA, Wampana B, Lateef GI, Lawan FA, Sanda KA, Thaluvwa AB,  Njobdi AN, Yagana KZ. 2013. Preliminary phytochemical and in vitro antibacterial evaluation of crude pericarp extract of Hyphaene thebaica(Doumpalm). Journal of Medicinal Plant and Herbal Therapy Research (JMPHTR) 1, 1-7.

Casal M, Vaquero M, Rinder H, Tortoli E, Grosset J, Gutierrez J, Jarlier V. 2005. A case study for multi-drug resistant tuberculosis risk factor in four European countries.MicrobialDrug Resistance 11, 62-67.

Chambers HF. 2001. The changing epidemiology of Staphylococcus aureus. Emerging Infectious Diseases 7, 178-182.

Dekanski D, Janicijevic-Hudomal S, Tadic V, Markovic G, Arsic I,Mestrovic D. 2009. Photochemical analysis and gastroprotective activity of an olive leaf extract.Journal ofSerbian Chemical Society 74, 367-377.

Fazal H, Ahmad N, Ulah I, Inayat H, Khan L, Abbasi BH. 2011. Antibacterial  potential in Parthenium hysterophorus, Stevia rebaudiana and Ginkgo biloba. Pakistan Journal of Botany 43, 1307-1313.

Gopalakrishnan S, Rajameena R, Vadivel E. 2012. Antimicrobial activity of the leaves of Myxopyrum serratulum A.W.Hill. International Journal of Pharmaceutical Sciences and Drug Research 4, 31-34.

Greathead W. 2003. Extraction and antimicrobial effect of Thai herbs and spices extracts.J. Agricultural and Food Chemistry 43, 361-371.

Grohmann F. 1974. Oleacceae. In: Flora of Pakistan. No.59.

Haloui E,  Marzouk Z,  Marzouk B, Bouftira I,  Bouraoui A, Fenina N. 2010. Pharmacological activities and chemical composition of the Olea europaea leaf essential oils from Tunisia.Journal of Food, Agriculture & Environment 2, 204-208.

Hansen K.1996. Isolation of an Angiotensin Converting Enzyme (ACE) inhibitor from Olea europaea and Olea valancia. Phytomedicine 2, 319-325.

Hussain A, Qarshi IA, liaqat R, Akhtar S, Aziz I, Ullah I, Shinwari K. 2014. Antimicrobial potential of leaf and fruit extracts and oils of wild and cultivated edible Olive. Pakistan Journal of Botany 46, 1463-1468.

Jorgensen JH, Turnidge JD. 2007. Susceptibility test methods: Dilution and disk diffusion methods. In: Murray, (Eds.), Manual of Clinical Microbiology. (9th Ed), ASM Press, Washington D.C., 1152-1172 p.

Khan MY, Panchal S, Vyas N, Butani A, Kumar V. 2015. Olea europaea: A phyto-pharmacological review. Pharmacognosy Review 1, 1.

Khan R, Islam B,  Akram M,  Shakil S,  Ahmad A, ManazirAS, Siddiqui M, khan AU. 2009. Antimicrobial activity of five herbal extracts against multi drug resistant (MDR) strains of bacteria and fungus of clinical origin. Molecules 14, 586-597.

Korukluoglu M, Sahan Y, Yigit A, Gucer S. 2010. Antibacterial activity and chemical constitiutions of Olea europaea L. leaf extracts. Journal of Food   processing and Preservations 34, 383-396.

KunhachanP,BanchonglikitulC, Kajsongkram T, Khayung A, Leelamanit W. 2012. Chemical composition, toxicity and vasodilation effect of the flowers extract of Jasminumsambac (L.) Ait. Evidence- Based Complementary and Alternative Medicine 4, 471312.

Mahmood A, Ahmad M, Jabeen A, Zafar M, Nadeem S. 2003.  Pharmacognostic studies of some indigenous medicinal plants of Pakistan. Journal of Ethnobotanical LeafletsSIUC, USA.

Majori MC. 1999. Plant products as antimicrobial agents. Clinical Microbiology Reviews 12, 564-582.

Micol V, Chaturla N, Fons LP, Mas V, Perez L, Estepa A. 2005. The olive leaf extract exhibits antiviral-activity against viralhaemorrhagic septicaemia rhabdovirus (VHSV). Antiviral Research 66, 129-136.

Nastro A, Germano MP, Angelo VD, Marino A, Cannatelli MA. 2000. Extraction    methods and bioautography for evaluation of medicinal plant antimicrobial activity. Letters in Applied  Microbiology 30, 379-384.

Paiva-Martins F, Gorden MH,Gameiro P. 2003. Activity and location of olive oil phenolic antioxidants in liposomes. Chemistry and Physics of Lipids 124, 23-36.

Pelczar MJ, Chan ECS, Krieg NR. 1993. Microbiology, Int. Ed.McGraw. 578.

Rybalchenko NP, Prykhodko VA, Nagorna SS, Volynets NN, OstapchukAN, Klochko VV, Rybalchenko TV, Avdeeva LV. 2010. In vitro antifungal activity of phenylheptatriyne from Bidens cernua L. against yeasts. Fitoterapia 81, 336-338.

Sabharwal S, Vats M, Sardana S, Aggarwal S. 2013. Pharmacological, Physico and Phytochemical evaluation of the leaves of Jasminum sambac Linn. InternationalJournalofPharmacy and Pharmaceutical Science ISSN: 0975-1491.

Samuelsson G. 2004. Drugs of Natural Origin: a textbookofPharmacognosy,5thSwedish. Pharmaceutical Press, Stockholm.

Shabir H, Khan MS, Rehman HU, Masood Z, Yousaf T, Majid A, Iqbal R.2015. Ethnomedicinal uses of xeric flora in tehsil Banda Daud Shah collected from district Karak KPK Pakistan. World Journal of Zoology 10, 59-69.

Steel RGD, Torrie JH, Discky DA. 1997. Principles and Procedures of Statistics: A Biometrical Approach. (3rd Ed.), McGraw Hill Co., New York.

Vasconsuelo A, Boland R. 2007. Molecular aspects of the early stages of elicitation of secondary metabolites in plants. Plant Science 172, 861-875.

Zhang ZYJ, Liu LYQ, Pu PX, Yang YC. 2008. Iridoidal glycosides from Jasminum sambac. Phytochemistry 38, 899-903.