Evaluation of in vitro fertilization outcome from very low sperm count and microdissection Testicular Sperm Extraction in patients with non-obstructive azoospermia: Paternal age factor
Paper Details
Evaluation of in vitro fertilization outcome from very low sperm count and microdissection Testicular Sperm Extraction in patients with non-obstructive azoospermia: Paternal age factor
Abstract
The study focused on evaluating the results of in vitro fertilization from very low sperm count and sperm from testicular microsurgery in NOA patients from different ages. Study subjects were divided into 2 groups: under or above 35 years old group; and each group was divided into 2 subgroups: T-ICSI and E-ICSI. The evaluation criteria mainly focused on the fertilization rates, the good embryo rates and the clinical pregnancy rates. The results showed that, in the under 35 years old group, the fertilization rates in the T-ICSI group were significantly higher than the E-ICSI group. However, the third day good embryo rates, the blastocyst formation and the good blastocyst rates in the both T-ICSI group and the E- CSI group were similar. In the above 35 years old group, the fertilization rates, the third day good embryo and the good blastocyst rates in the T-ICSI group were significantly higher than E-ICSI group The percentage of live births in the T-ICSI group was higher than the E-ICSI group, but there was no statistical difference. Especially, the weight of children born from the group using sperm from microTESE was heavier than that of children born from the group of sperm from Cryptozoospermia. The results of the study showed that sperm extraction from testicular microsurgery improved embryology results in the above 35 years old of males with non-obstructive azoospermia. Regarding clinical treatment results, the group using sperm from testicular microsurgery tended to be higher than the group using sperm from ejaculate samples.
Alpha Scientists in Reproductive M, Embryology ESIGO. 2011. The Istanbul consensus workshop on embryo assessment: proceedings of an expert meeting. Hum Reprod 26(6), 1270-1283. http://dx.doi.org/10.1093/humrep/der037.
Alkandari MH, Bouhadana D, Zini A. 2021. Is a contralateral testicular exploration required at microdissection testicular sperm extraction for men with nonobstructive azoospermia, cryptozoospermia or severe oligozoospermia? Andrologia 53(11), e14208. http://dx.doi.org/10.1111/and.14208.
Almajed, W., M. Alharbi and A. Zini 2020. Use of mini-incision microdissection testicular sperm extraction in men with cryptozoospermia and non-obstructive azoospermia. Andrology 8(5), 1136-1142. http://dx.doi.org/10.1111/andr.12795.
Ben-Ami I, Raziel A, Strassburger D, Komarovsky D, Ron-El R, Friedler S. 2013. Intracytoplasmic sperm injection outcome of ejaculated versus extracted testicular spermatozoa in cryptozoospermic men. Fertil Steril 99(7), 1867-1871. http://dx.doi.org/10.1016/j.fertnstert.2013.02.025.
Bradley CK, McArthur SJ, Gee AJ, Weiss KA, Schmidt U, Toogood L. 2016. Intervention improves assisted conception intracytoplasmic sperm injection outcomes for patients with high levels of sperm DNA fragmentation: a retrospective analysis. Andrology 4(5), 903-910. http://dx.doi.org/10.1111/andr.12215.
Cui X, Ding P, Gao G, Zhang Y. 2017. Comparison of the Clinical Outcomes of Intracytoplasmic Sperm Injection Between Spermatozoa Retrieved From Testicular Biopsy and From Ejaculate in Cryptozoospermia Patients. Urology 102, 106-110. http://dx.doi.org/10.1016/j.urology.2016.08.071.
Elbardisi H, Arafa M, Singh N, Betts B, Agrawal A, Henkel R, Al-Hadi AA, Burjaq H, Alattar A, Khalafalla K, Majzoub A. 2021. The effect of paternal age on intracytoplasmic sperm injection outcome in unexplained infertility. Arab Journal of Urology 19(3), 274-280. http://dx.doi.org/10.1080/2090598x.2021.1955553.
Esteves SC, Sánchez-Martín F, Sánchez-Martín P, Schneider DT, Gosálvez J. 2015. Comparison of reproductive outcome in oligozoospermic men with high sperm DNA fragmentation undergoing intracytoplasmic sperm injection with ejaculated and testicular sperm. Fertil Steril 104(6), 1398-1405. http://dx.doi.org/10.1016/j.fertnstert.2015.08.028.
Esteves SC, Santi D, Simoni M. 2020. An update on clinical and surgical interventions to reduce sperm DNA fragmentation in infertile men. Andrology 8(1), 53-81. http://dx.doi.org/10.1111/andr.12724.
Franco G, Scarselli F, Casciani V, De Nunzio C, Dente D, Leonardo C, Greco PF, Greco A, Minasi MG, Greco E. 2016. A novel stepwise micro-TESE approach in non obstructive azoospermia. BMC Urol 16(1), 20. http://dx.doi.org/10.1186/s12894-016-0138-6.
Haimov-Kochman R, Lossos F, Nefesh I, Zentner BS, Moz Y, Prus D, Bdolah Y, Hurwitz A. 2009. The value of repeat testicular sperm retrieval in azoospermic men. Fertil Steril 91(4 Suppl): 1401-1403. http://dx.doi.org/10.1016/j.fertnstert.2008.04.066.
Hồ Mạnh Tường, Đặng Quang Vinh and Vương Thị Ngọc Lan 2020. Thụ Tinh Trong Ống Nghiệm, NXB Y học.
Huỳnh Thị Thu Thảo and Tuyết HTD. 2020. The rate of successful sperm collection from microscopic testicle extraction and related factors at Hanh Phuc international hospital, Pham Ngoc Thach Medical University.
Jin, L., Z. Li, L. Gu and B. Huang 2020. Neonatal outcome of children born after ICSI with epididymal or testicular sperm: A 10-year study in China. Sci Rep 10(1), 5145. http://dx.doi.org/10.1038/s41598-020-62102-y.
Lan KC,. Huang FJ, Lin YC,. Kung FT, Hsieh C. H, Huang HW, Tan PH, Chang SY. 2003. The predictive value of using a combined Z-score and day 3 embryo morphology score in the assessment of embryo survival on day 5. Hum Reprod 18(6), 1299-1306. http://dx.doi.org/10.1093/humrep/deg239.
Moskovtsev SI, Jarvi K, Mullen JB, Cadesky KI, Hannam T, Lo KC. 2010. Testicular spermatozoa have statistically significantly lower DNA damage compared with ejaculated spermatozoa in patients with unsuccessful oral antioxidant treatment. Fertil Steril 93(4), 1142-1146. http://dx.doi.org/10.1016/j.fertnstert.2008.11.005.
Ostad M, Liotta D, Ye Z, Schlegel PN. 1998. Testicular sperm extraction for nonobstructive azoospermia: results of a multibiopsy approach with optimized tissue dispersion. Urology 52(4), 692-696. http://dx.doi.org/10.1016/s0090-4295(98)00322-7.
Sánchez-Rubio F, Soria-Meneses PJ, Jurado-Campos A, Bartolomé-García J, Gómez-Rubio V, Soler AJ, Arroyo-Jimenez MM, Santander-Ortega MJ, Plaza-Oliver M, Lozano MV,. Garde JJ, Fernández-Santos MR. 2020. Nanotechnology in reproduction: Vitamin E nanoemulsions for reducing oxidative stress in sperm cells. Free Radical Biology and Medicine 160, 47-56. http://dx.doi.org/10.1016/j.freeradbiomed.2020.07.024.
Showell MG, Mackenzie-Proctor R, Brown J, Yazdani A, Stankiewicz MT, Hart RJ. 2014. Antioxidants for male subfertility. Cochrane Database Syst Rev (12), Cd007411. http://dx.doi.org/10.1002/14651858.CD007411.pub3
Tournaye H, Camus M, Goossens A, Liu J, Nagy P, Silber S, Van Steirteghem AC, P. Devroey 1995. Recent concepts in the management of infertility because of non-obstructive azoospermia. Hum Reprod 10(1), 115-119. http://dx.doi.org/10.1093/humrep/10.suppl_1.115.
Vahidi S, Narimani N, Abouei S, Sadeghi A, Lorian K, Rahavian A. 2021. Comparison of intracytoplasmic sperm injection outcomes in azoospermic men who underwent testicular sperm extraction vs. microdissection testicular sperm extraction: A cross-sectional study. International Journal of Reproductive BioMedicine 19(9), 837-844. http://dx.doi.org/10.18502/ijrm.v19i9.9716.
Watanabe S. 2022. DNA damage in human sperm: The sperm chromosome assay. Reproductive Medicine and Biology 21(1), e12461. http://dx.doi.org/10.1002/rmb2.12461.
Wdowiak A, Bakalczuk S, Bakalczuk G. 2015. The effect of sperm DNA fragmentation on the dynamics of the embryonic development in intracytoplasmatic sperm injection. Reproductive Biology 15(2), 94-100. http://dx.doi.org/10.1016/j.repbio.2015.03.003.
WHO. 2021. WHO laboratory manual for the Examination and processing of human semen.
Yu Y, Wang R, Xi Q, Zhang H, Jiang Y, Li L, Liu R, Zhang X. 2019. Effect of paternal age on intracytoplasmic sperm injection outcomes in cryptozoospermic men: Ejaculated or testicular sperm? Medicine (Baltimore) 98(26), e16209. http://dx.doi.org/10.1097/md.0000000000016209.
Thong Nguyen Quang, Tu Nguyen Huynh Cam, Khoi Tran Vy, An Vo Thien, Thao Huynh Thi Thu, Huyen Nguyen Thi Thuong (2022), Evaluation of in vitro fertilization outcome from very low sperm count and microdissection Testicular Sperm Extraction in patients with non-obstructive azoospermia: Paternal age factor; IJB, V21, N6, December, P130-139
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