Evaluation of Kenyan wheat (Triticum aestimum L.) genotypes for leaf rust (Puccinia triticina Eriks.) at adult plant stage

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Evaluation of Kenyan wheat (Triticum aestimum L.) genotypes for leaf rust (Puccinia triticina Eriks.) at adult plant stage

Anncetta Gakii Nyamu, James O. Owuoche, Pascal P.O Ojwang, Ruth Wanyera
Int. J. Agron. Agri. Res.11( 6), 66-78, December 2017.
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Leaf rust (Puccinia triticina) is one of the major rust diseases that affect wheat (Triticum aestivum) production worldwide. The objective of this study was to determine genotypic variation among Kenyan wheat genotypes against leaf rust at adult plant stage. A set of 144 genotypes were evaluated in a two-season field experiments at Kenya Agricultural and Livestock Research Organization (KALRO), Njoro. In the field, genotypes were sown in 12 12 partially balanced lattice design. Adult plant infection assessed by Area under Disease Progress Curve ranged from means of 42.00 to 145.00. Mean grain yield ranged from 0.06 to 6.81 tonnes. ha-1. Highly significant (p ≤ 0.001) variations were noted among the seasons, genotypes tested over seasons and the interaction between genotype  season for plant height, a thousand kernel weight (TKW), and harvest index. There were significant (p ≤ 0.01) effects due to seasons and genotypes for spike length, days to maturity, leaf rust infection and grain filling period, biomass, yield, respectively. Effects due to seasons were significant (p ≤ 0.05) for hectoliter weight and stem rust infection. Genotypes K. Tai, K. Korongo, Fletcher, Verder, R1244, R1301 and R1305 exhibited adult plant resistance in both seasons. Considering the disease response and yield potential, genotypes R1301 and R1305 showed lowest leaf rust infection and highest grain yield. These genotypes are suitable candidates for utilization in yield and leaf rust resistance improvement programmes in Kenya.


Arslan U, Yagdi K, Aydogan E. 2002. Reactions of some bread wheat against leaf rust (Puccinia triticina Eriks) under Bursa conditions and related yield losses. Journal of Uludag University 16, 201-210.

Bancal MO, Robert C, Ney B. 2007. Modelling wheat growth and yield losses from late epidemics of foliar diseases using loss of green leaf area per layer and pre-anthesis reserves. Annals of Botany 100, 777-789.

Bhatta M. 2015. “Effect of Genotype, Environment, and Production Packages on Yield, Agronomic Characteristics, and End-Use Quality of Winter Wheat” Theses, Dissertations, and Student Research in Agronomy and Horticulture. Paper 98. http://digitalcommons.unl.edu/agronhortdiss/98.

Bolton MD, Kolmer JA, Galvin DF. 2008. Wheat leaf rust caused by Puccinia triticina, Molecular plant pathology 9, 563-575.

Brown JK, Hovmoller MS. 2011. Aerial dispersal of pathogens on the global and continental scales and its impact on plant disease, Science 297, 537-548.

Campbell CL, Madden VL. 1990. Introduction to Plant Disease Epidemiology. John Willy and Sons, New Yolk City.

Cherukuri DP, Gupta SK, Charpe A, Koul S, Prabhu KV, Singh RB, Haq QMR. 2005. Molecular mapping of Aegilops speltoides derived leaf rust resistance gene Lr28 in wheat. Euphytica 143, 19-26.

CIMMYT. 2008. A programme for calculation of AUDPC. Mexico DF a software package. CIMMYT, Mexico.

Draz IS, Abou-Elseoud MS, Kamara AM, Alaa-Eldein OA, El-Bebany AF. 2005. Screening of wheat genotypes for leaf rust resistance along with grain yield. Annals of Agricultural Science 60, 29-39.

Fayyaz M, Rattu AR, Ahmad I, Akhtar MA, Hakro AA, Mujeeb-Kazi A. 2008. Current status of the occurrence and distribution of (Puccinia triticina) Wheat leaf rust virulence in Pakistan. Journal of Botany 40, 887-895.

Feuillet C, Travella S, Stein N, Albar L, Nubiat L, Keller B. 2003. Map-based isolation of the leaf rust disease resistance gene Lr10 from the hexaploid wheat (Triticum aestivum L.) genome. Proceedings of the National Academy of sciences USA 100, 15253-15258.

German S, Barcellos A, Chaves M, Kohli M, Decompos P, Viedma L. 2007. The situation of common wheat rusts in the southern core of America and perspectives for control. Australian Journal of Agricultural Research 58, 620-630.

Hailegiorgis DM Genet T. 2011. Genetic Divergence Analysis on some Bread Wheat Genotypes Grown in Ethiopia. Journal of Central European Agriculture 12, 344-352. http;//mathworld.Wolfran. com/correlationcoefficient.html. Retrieved on 20th, April 2017.

Huang L, Brooks SA, Li W, Feller JP, Trick HN, Gill BS. 2003. Map-based cloning of leaf rust resistance gene Lr21 from the large and polyploid genome of wheat. Genetics 164, 655-664.

Huerta-Espino J, Singh V, German S, McCallum B, Park R F, Chen WQ, Bhardwaj SC, Goyeau H. 2011. Global status of wheat leaf rust caused by Puccinia triticina, Euphytica 179, 143-160.

Hussain M, Chaudhry MH, Rehman A, Anwar J, Khan SB. 1999. Development of durable rust resistance in wheat. Phytopathology 11, 130-139.

Hysing SC, Singh RP, Huerta-Espino J, Merker A, Lilje-roth E, Diaz O. 2006. Leaf rust (Puccinia triticina) resistance in wheat (Triticum aestivum) cultivars grown in Northern Europe 1992-2002. Hereditas 143, 1-14.

Jaetzold R, Hornetz B, Shisanya CA, Schmidt H. 2012. Farm Management Handbook of Kenya. Volume 4 (Western, Central, Eastern, Nyanza, Southern Rift Valley, Northern Rift Valley, Coast), Nairobi. Available at: www.uni-trier.de/index.php?id=58581.

Johnston CO, Browder LE. 1966. Seventh revision of the International register of physiologic races of Puccinia recondite f. sp. Tritici. Plant disease 50, 756-760.

Kolmer JA, Long DL, Hughes ME. 2005. Physiological specialization of Puccinia triticina on wheat in the United States in 2003. Plant disease 89, 1201-1206.

Kolmer JA. 1996. Genetics of resistance to wheat leaf rust. Annual Review of phytopathology 34, 435-455.

Kolmer JA. 2005. Tracking wheat rust on a continental scale, Plant Biology 8, 441-449.

Li ZF, He ZH, Li X, Zhang LJ, Wang HY, Meng QF, Yang WX, Li G, Liu DQ. 2010. Seedling and Slow Rusting Resistance to Leaf Rust in Chinese Wheat Cultivars. Plant Disease 94, 45-53.

Martinez F, Niks RE, Singh RP, Rubiales D. 2001. Characterization of Lr46, gene conferring partial resistances to wheat leaf rust. Journal of Heredity 135, 111-114.

McDonald BA, Linde C. 2002. Pathogen population genetics, evolutionary potential, and durable resistance. Annual Review Phytopathology 40, 349-379.

McIntosh RA, Yamazaki Y, ubcovsky J, Rogers WJ, Morris CF, Somers DJ. 2008. Catalogue of gene symbols for wheat. Proceedings of the 11th International Wheat Genetics Symposium. Brisbane, Australia.

Milan M, Novo P, Peter C, Vojislari M, Vladimir A, Bojan J, Nicola H, Novica M. 2015. Relationship between grain yield and agronomic traits in winter barley. Crop Science 1, 124-134.

Mirza JM, Ahmad F, Abdul M. 1992. Correlation study and path Analysis of plant height, yield and yield components. Journal of Agriculture 8, 647-651.

Mojerlou S, Safaie N, Alizadeh A, Khelghatibana F. 2009. Measuring and modeling crop loss of wheat caused by septoria leaf blotch in seven cultivars and lines in Iran. Journal of Plant Production Research 9, 3-8.

Namibi A, Tewari JP, Singh R, McCallum B, Laroche A, Briggs. 2005. Inheritence and QTL analysis of durable resistance to stripe and leaf rust in an Australian cultivars, Triticum aestivum. Genome 48, 97-107.

Naser S, Masoud SB, Fida A, Farzad A, Ebrahim M G, Kumarse N, Samer L, Seyed TD. 2013. Molecular genetic diversity in Iranian populations of Puccinia triticina, the causal agent of wheat leaf rust. Plant Science 4, 1375-1386.

Newcomb M, Acevedo M, Bockelman HE, Brown, Guedira G, Goates BJ, Jackson EW, Jin Y, Njau P, Rouse MN, Singh D, Wanyera R, Bonman JM. 2013. Field resistance to the Ug99 race group of the stem rust pathogen in spring wheat landraces. Plant disease 97, 882-890.

Ntanos DA, Koutroubas SD. 2002. Dry matter and N accumulation and translocation for Indica and Japonica rice under Mediterranean conditions. Crops Research 74, 93-101.

Nzuve FM, Bhavani S, Tusiime G, Njau P, Wanyera R. 2012. Evaluation of bread wheat for both Seedling and Adult Plant resistance to stem rust. Plan science 6, 426-432.

Ochoa J, Parlevliet JE. 2007. Effect of partial resistance to barley leaf rust, Puccinia hordei on the yield of three barley cultivars. Euphytica 153, 309-312.

Ormoli L, Costa C, Negri S, Perenzin M, Vaccino P. 2005. Diversity trends in bread wheat in Italy during the 20th century assessed by traditional and multivariate approaches, Scientific reports 5, 8574-8581.

Peterson RF, Campbell AB, Hannah AE. 1948. A diagrammatic scale for estimating rust intensity of leaves and stem of cereals. Canadian Journal of Research 26, 496-500.

Purnima S, Sundeep K, Uttarn K, Laksman P, Amit KS, Rakesh S, Joshi AS. 2012. Pathological and molecular characterizations of slow rusting in fifteen wheat (Triticum aestivum Lem Thell) genotypes. African journal of biotechnology 11, 14956-14966.

Rubiales D, Nick RE. 2000. Combination of mechanism of resistance to rust fungi as a strategy to increase durability. CHEM-IAMZ 6, 333-339.

Singh D, Park RF, McIntosh. 2001. Postulation of leaf (Brown) rust resistance genes in 70 wheat cultivars grown in the United Kingdom. Euphytica 120, 205-218.

Singh RP, Huerta-Espino J, Rajaram S. 2000. Achieving near-immunity to leaf and stripe rusts in wheat by combining slow rusting resistance genes. Phytopathology 35, 133-139.

Singh RP, Huerta-Espino J, William M. 2001. Slow rusting genes based resistance to leaf and yellow rusts in wheat: genetics and breeding at CIMMYT. Proceedings of the 10th Assembly of the Wheat Breeding Society of Australia, 16th-21st September 2001, Mildura, Australia pp. 103-108.

Steel R, Torrie J. 1980. Principles and procedures of statistics-a biometrical approach. 2nd edition. McGraw-Hill, New York.

Watson IA, Luig NH. 1983. Progressive increase in virulence in Puccinia graminis f. sp. tritici. Phytopathology 58, 70-73.

William M, Singh RP, Huerta-Espino J, Ortiz Islas S, Hoisington D. 2003. Molecular marker mapping of leaf rust resistance gene Lr46 and its association with stripe rust resistance gene Yr29 in wheat. Phytopathology 93, 153-159.

Yan Z, Joseph GL, Roger B, Natalia L. 2010. Effects of genotype by environment interaction on agronomic traits in soybean. Crop Science 50, 696-702.

Yuen J, Dlurle A, Crossa J, Huerrera-Foessel A S, Singh RP, Huerta- Espino J. 2007. Evaluation of slow rusting resistance components to leaf rust in CIMMYT durum wheats. Euphytica 1, 69-72.

Zadoks JC, Chang TT, Konzak CF. 1974. A decimal code for the growth stages of cereals. Weed Research 14, 415-421.