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Evaluation of larvicidal potential of Azadirachta indica (Neem) plant extract and synthesized AgNPs against Aedes aegypti L. (Diptera: Culicidae)

Research Paper | April 1, 2019

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Muhammad Waris, Shabab Nasir, Farhat Jabeen, Tayyaba Sultana

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Int. J. Biosci.14( 4), 26-34, April 2019

DOI: http://dx.doi.org/10.12692/ijb/14.4.26-34


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Aedes mosquitoes are the most important group of vectors having ability to cause diseases like Chikungunya fever, Dengue fever and Zika virus in human. These vectors can efficiently and ecofriendly be controlled by using green silver nanoparticles (AgNPs). In the present study, leaves extract of Azadirachta indica (Neem) and green silver nanoparticles (AgNPs) synthesized from this extract were evaluated as larvicidal agent for 2nd  and 3rd instar larvae of the Aedes aegypti ( main mosquito vectors). UV-Vis spectroscopy, X-ray spectroscopy (XRD), Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM) were used to study the characteristics of green synthesized AgNPs.2nd and 3rd instar larvae of Aedes aegypti were kept in the different concentrations (50-250ppm) of plant extract and green AgNPs to calculate the percentage mortality at time intervals of 12, 24, 36 and 48h of exposure. The nanoparticles proved significant toxic for the Ae. aegypti larvae (dengue vector) having (LC50 =15.94 and LC90 =111.82 ppm) compared to the plant extract (102.46 ppm; 251.80 ppm) respectively. Our results suggest the extract of A. indica and synthesized nanoparticles as excellent controlling agents for vector mosquitoes instead of pollution causing existing chemical pesticides.


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