Int. J. Micro. Myco.4(2), 1-7, September 2016
Environmental contamination for excessive use of chemical protectant increasing the interest in integrated pest management and organic farming. Trichoderma harzianum (Rifai) a promising biocontrol agent fairly acceptable globally used against wide array of plant pathogens. Culture establishment and easy utilization technique of those isolates may not evident for commercialization in all level because of costly production. To cultivate the inoculums in the cheapest suitable media of organic source present study was undertaken. Mycelial growth, conidial production and biomass yield of Trichoderma harzianum were examined on four different culture media including potato dextrose agar, modified potato dextrose agar, carrot Agar, pulse sucrose agar. The medium had a significant effect on growth rate and population of the Trichoderma species. Carrot Agar was the best medium in terms of quick growth rate and spore production with at low cost. Average linear growth rate was measured after three days of inoculation and highest linear growth has found on potato dextrose agar medium followed by carrot agar. The Biomass yield also recorded as fresh weight and dry weight of inoculums from liquid culture medium and the maximum yielding of inoculums has found in potato dextrose broth and minimum in carrot broth.
Das BC, Roy SK, Boro LC. 1997. Mass multiplication of Trichoderma species on different media. Ind. J. Agri. Sci. 10(1), 95-100.
Elad Y, Chet I, Henis Y. 1981. A selective medium for improving quantitative isolation of Trichoderma spp. from soil. Phytoparasitica 9(1), 59-69.
Harman GE, Howell CR, Viterbo A, Chet I, Lorieto M. 2004. Trichoderma species opportunistic, a virulent plant symbionts. Nat. Rev. 2, 43-56.
Harman GE, Jin X, Stasz TE, Peruzzotti G, Leopold AC, Taylor AG. 1991. Production of conidial biomass of Trichoderma harzianum for biological control. Biological Control 1, 23-28.
Harman GE. 2006. Overview of mechanisms and uses of Trichoderma spp. Phytopatho. 96, 190-194.
Herrera-Estrella A, Chet I. 2003. The biological control agent Trichoderma: from fundamentals to applications. In: Arora, D. (Ed), Hand book of fungal Biotechnology, Dekker, New York, pp1000-10020.
Jahan N, Sultana S, Adhikary SK, Rahman S, Yasmin S. 2013. Evaluation of the growth performance of Trichoderma harzianum (Rifai) on different culture media. Journal of Agriculture and Veterenary Science 3(4), 44-50.
Khandelwal M, Datta S, Mehta J, Naruka R, Makhijani K, Sharma G, Kumar R, Chandra S. 2012. Isolation, characterization & biomass production of Trichoderma viride using various agro products- A biocontrol agent. Advance in Applied Science Research 3(6), 3950-3955.
Kumhar KC, Babu A, Bordoloi M, Ali A. 2014. Evaluation of culture media for biomass production of Trichoderma viride (KBN 24) and their production economics. American Journal of Agriculture and Forestry 2(6), 317-320.
Mustafa M, Khan MA, Inam-ul-Haq M, Aslam Pervez M, Umar U. 2009. Usefullness of different culture media for in vitro evaluation of Trichoderma spp. against seed born fungi of economic importance. Pak. J. Phytopathol 21(1), 83-88.
Shoresh M, Harman GE, Mastoury F. 2010. Induced systemic resistance and plant responses to fungal biocontrol agent. Ann. Rev. Phytopathol 48, 21-43.
Sing A, Shahid M, Srivastava M, Panday S, Sarma A, Kumar V. 2014. Optimal physical parameters for growth of Trichoderma species at varying PH, Temperature and agitation. Virol Mycol 3, 127. DOI: 10,4172/2161-0517.1000127.
Subash N, Viji J, Sasikumar C, Meenaks-hisundaram M. 2013. Isolation, media optimization and formulation of Trichodermaharizanumin agricultural soil. Microbiol. Biotech. Res 1, 61-64.