Evaluation of PGPR strains having ACC deaminase activity for their efficacy to induce water stress tolerance in sunflower under varied moisture regimes

Paper Details

Research Paper 01/02/2019
Views (359) Download (14)

Evaluation of PGPR strains having ACC deaminase activity for their efficacy to induce water stress tolerance in sunflower under varied moisture regimes

Muhammad Shahid Siddique, Ghulam Qadir, Shahid Maqsood Gill, Tariq Sultan, Riffat Hayyat
Int. J. Biosci.14( 2), 418-428, February 2019.
Certificate: IJB 2019 [Generate Certificate]


Sunflower is an important oil seed crop in Pakistan but incidence of drought stress considerably hampers its growth and productivity. The present pot study was performed to evaluate the potential of PGPR strains having ACC deaminase activity for their efficacy to induce water stress tolerance in sunflower. Ten PGPR strains (KS7, KS8, KS10, KS15, KS17, KS25, KS28, KS41, KS42 and KS44) already isolated and identified (Kiani et al., 2015) along with uninoculated control were tested at three soil moisture levels (25, 20 and 15% volumetric water contents representing no, medium and high soil water stress, respectively) in a two factor Completely Randomized Design with three replications. The volumetric water contents were determined daily with Time Domain Reflectometer (TDR) and different soil moisture regimes were maintained accordingly. Four plants were maintained in each pot. Two plants each after 30 and 60 days of germination were harvested for data recorded. The results revealed that at high water stressed condition (15%VWC),KS42 and KS17 caused maximum increase of upto 35% and 31%, respectively in chlorophyll SPAD readings over uninoculated control after 30 and 60 DAG. While in terms of growth traits PGPR strains KS42 and KS7 outperformed and gave maximum increment of upto 151% &104%, 255% & 205% and 139% & 100% in fresh & dry shoot weight, fresh & dry root weight and total root length, respectively over uninoculated control. It was concluded that PGPR strains KS42 and KS7 exhibited greater potential for mitigation of negative effects of drought stress in sunflower.


Arshad M, Shaharoona B, Mahmood T. 2008. Inoculation with Pseudomonas spp. containing ACC-deaminase partially eliminates the effects of drought stress on growth, yield, and ripening of pea (Pisum sativum L.). Pedosphere 18(5), 611-620. https://doi.org/10.1016/S1002-0160(08)60055-7

Ambrosini A, Beneduzi A, Stefanski T, Pinheiro FG, Vargas LK, Passaglia LMP. 2012. Screening of plant growth promoting Rhizobacteria isolated from sunflower (Helianthus annuus L.). Plant Soil 356(2), 245–264. http://dx.doi.org/10.1007/s11104-011-1079-1

Badar H, Javed MS, Ali A, Batool Z. 2002. Production and Marketing Constraints Limiting Sunflower Production in Punjab (Pakistan). International journal of Agriculture and Biology 4(2), 267-271.

Bangash N, Khalid A, Mahmood T, Siddique MT. 2013. Screening rhizobacteria containing ACC-deaminase for growth promotion of wheat under water stress. Pakistan Journal of Botany 45, 91-96

Chen L, Dodd IC, Davies WJ, Wilkinson S. 2003. Ethylene limits abscisic acid- or soil drying-induced stomatal closure in aged wheat leaves. Plant, Cell and Environment 36(10), 1850–1859. https://doi.org/10.1111/pce.12094

Debaeke P, Abdellah A. 2004. Adaptation of crop management to water limited environments. European Journal of Agronomy 21(4), 433–446. https://doi.org/10.1016/j.eja.2004.07.006

Edmeades GO. 2008. Drought tolerance in maize: an emerging reality. A feature. In: James, C. (Ed.), Global Status of Commercialized Biotech/GM Crops. ISAAA Brief 39, 197-217.

Fulda S, Mikkat S, Stegmann H, Horn R. 2011. Physiology and proteomics of drought stress acclimation in sunflower (Helianthus annuus L.). Plant Biology 13(4), 632-642. https://doi.10.1111/j.1438-8677.2010.00426.x.

Glick BR, Cheng Z, Czarny JC, Duan J. 2007. Promotion of plant growth by ACC-deaminase containing soil bacteria. European Journal of Plant Pathology 119, 329-339. http://dx.doi.org/10.1007/sI0658-007-9162-4

GOP. “Economic Survey of Pakistan 2014-2015”. Government of Pakistan, Ministry of Food, Agriculture and Livestock, Agriculture & Livestock Division (Economic Wing), Islamabad.

Huang Y, Hutchison LH, Laskey CE, Kieber JJ. 2003. Biochemical and functional analysis of CTR1, a protein kinase that negatively regulates ethylene signaling in Arabidopsis. The Plant Journal 33(2), 221-233. https://doi.org/10.1046/j.1365-313X.2003.01620.x

Hardoim PR, Van Overbeek LS, Van Elsas JD. 2008. Properties of bacterial endophytes and their proposed role in plant growth. The Trends in Microbiology 16(10), 463–471. https://doi.10.1016/j.tim.2008.07.008

Iqbal N. 2004. Influence of exogenous glycine betaine on drought tolerance of sunflower (Helianthus annuus L.). Ph.D. Thesis, Dept. of Bot, Univ. of Agri., Faisalabad.

Khalil IA, Jan A. 2002. Cropping technology, National Book Foundation, Islamabad. Pakistan, p 28.

Kasim WA, Osman ME, Omar MN, Abd El-Daim IA, Beja S, Meijer J. 2013. Control of drought stress in wheat using plant growth-promoting bacteria. Journal of Plant Growth Regulation 32(1), 122–130.

Khalid A, Arshad M, Zahir ZA. 2004. Screening plant growth-promoting rhizobacteria for improving growth and yield of wheat. Journal of Applied Microbiology 96(3), 473–480. https://doi.org/10.1046/j.1365-2672.2003.02161.x

Kiani SP, Grieu P, Maury P,  Hewezit T, Gentzbittel A. Sarrafi S. 2007. Genetic variability for physiological traits under drought conditions and differential expression of water stress-associated genes in sunflower. Theory of Applied Genetics 114(2), 193-207.

Kiani SP, Maury P, Sarrafi S, Grieu P. 2008. QTL analysis of chlorophyll fluorescence parameters in sunflower (Helianthus annuus L.) under well-watered and water-stressed conditions. Plant Science 175(4), 565-573.

Kiani MZ, Ali A, Sultan T, Ahmed MM. 2015. Characterization of plant growth promoting rhizobacteria isolated from root system of sunflower. Pakistan Journal of Agricultural Research 28(2), 136-142.

Kang BG, Kim WT, Yun HS, Chang SC. 2010. Use of plant growth-promoting rhizobacteria to control stress responses of plant roots. Plant Biotechnology 4(3), 179-183. http://dx.doi.org/10.10.1007/s11816-010-0136-1

Kavamura VN, Santos SN, da Silva JL, Parma MM,  ÁvilaL A, Visconti A,  Zucchi TD, Taketani RG, Andreote FD, de Melo IS. 2013. Screening of Brazilian cacti rhizobacteria for plant growth promotion under drought. Microbiological Research 168(4), 183–191. https://doi.10.1016/j.micres.2012.12.002

Lauteri M, Haworth TD, Serrajn R, Monteverdi MC, Centritto M. 2014. Photosynthetic diffusional constraints affect yield in drought stressed rice cultivars during flowering. PLoS One 9(9), e109054.http://dx.doi.org/10.10.1371/journal.pone.0109054;10.1371/journal.pone.0117631

Maniatis T, Frtch EF, Sambook. 1982. Molecular Coloning; A Laboratory Manual. ColdSpring Harber laboratory, New York, USA., 545.

Manivannan P, Jaleel CA, Sankar B,  Kishorekumar A,  Somasundaram R, Alagu LGM, Panneerselvam R. 2007b. Growth, biochemical modifications and proline metabolism in Helianthus annuus L. as induced by drought stress. Colloids Surfaces B: Biointerfaces 59(2), 141-149. https://doi.10.1016/j.colsurfb.2007.05.002

Marasco R, Rolli E, Ettoumi B, Vigani G, Mapelli F, Borin S. 2012. A droughtresistance-promoting microbiome is selected by root system under desert farming. PLoS ONE 7, e48-479. http://dx.doi.org/10.10.1371/journal.pone.0048479

Nezami A, Khazaei HR, Rezazadeh ZB, Hosseini A. 2008. Effects of drought stress and defoliation on sunflower (Helianthus annuus L.) in controlled conditions. Desert 12(2), 99-104. http://jdesert.ut.ac.ir

Nain L, Rana A, Joshi M, Shrikrishna JD, Kumar D,  Shivay YS,  Paul S, Prasanna R. 2010. Evaluation of synergistic effects of bacterial and cyanobacterial strains as biofertilizers for wheat. Plant and Soil 331, 17–230

Nadeem SM, Zahir ZA, Naveed M, Ashraf M. 2013. Microbial ACC-deaminase: prospects and applications inducing salt tolerance in plants. Critical Reviews in Plant Sciences 29(6), 360-393.

Ommen OE, Donnelly A, Vanhoutvin S, Vanoijen M, Manderscheid R. 1999. Chlorophyll content of spring wheat flag leaves grown under elevated CO2 concentration and other environmental stress within `ESPACE-Wheat` project. European Journal of Agronomy 10(4), 197-203 http://dx.doi.org/10.10.1016/S1161-0301(99)00011-8

Rathore VS, Gautam RC, Kaushik SH. 2001. Yield, quality and nutrient uptake by sunflower as influenced by weed and nutrient management. Journal Annals of Agricultural Research 22(3), 443-444.

Ranawake AL, Dahanayaka N, Amarasingha UGS, Rodrigo WDRJ, Rodrigo UTD. 2011. Effect of water stress on growth and yield of mung bean (Vigna radiata L.). Tropical Agricultural Research and Extension 14(4), 76-79. http://www.agri.ruh.ac.lk/tare/pdf/V

Shaharoona B, Arshad M, Zahir ZA. 2006. Effect of plant growth promoting rhizobacteria containing ACC-deaminase on maize (Zea mays L.) growth under axenic conditions and on nodulation in mung bean (Vigna radiata L.). Letters in Applied Microbiology 42, 155-159.

Skoric D, Jocic S, Lecic, N. 2008. Genetic possibilities for altering sunflower oil quality to obtain novel oils. Canadian Journal of Physiology and Pharmacology 86(4), 215-221.

Skoric D. 2009. Sunflower breeding for resistance to abiotic stresses. Helia 32(50), 1-16.

Siddiqui MH. 2010. Nutrient management for sunflower production. PhD diss., Tendo Jam univ. Sindh, (Pakistan).

Sandhya V, Ali SKZ, Grover M, Reddy G, Venkateswarlu B. 2010. Effect of plant growth promoting Pseudomonas spp. on compatible solutes, antioxidant status and plant growth of maize under drought stress. Plant Growth Regulation 62(1), 21-30. https://doi.10.1007/s10725-010-9479-4

Stefan M, Munteanu N, Staler N, Mihasan M. 2013. Effects of inoculation with plant growth promoting rhizobacteria on photosynthesis, antioxidant status and yield of runner bean. Romanian Biotechnological Letters 18(2), 8132-8143.

Tank N, Saraf M. 2010. Salinity-resistant plant growth promoting rhizobacteria ameliorates sodium chloride stress on tomato plants. Journal of Plant Interaction 5(1), 51–58. https://doi.org/10.1080/17429140903125848

Vasudevan P, ReddyMS, Kavitha S, Velusamy P, Paul Raj RSD, Purushotta-man SM, Priyadarsini VB, Bharathkumar S,  Kloepper JW, Gnanamanickam SI. 2002. Enhancement of rice seedling growth and grain yield. Current Science 83, 1140–1143.

Zhao TJ,  Sun S, Liu Y,  Liu JM,  Liu Q, Yan YB, Zhou HM. 2006. Regulating thedrought-responsive element (DRE)-mediated signaling pathway by synergic functions of trans-active and transinactive DRE binding factors in Brassica napus. Journal of Biological Chemistry 281(16), 10752-10759.

Zahir ZA, Munir AHN, Shahroona B, Arshad M. 2008. Effectiveness of rhizobacteria containing ACC-deaminase for growth promotion of peas (Pisum sativum L.) under drought conditions. Journal of Microbiolology & Biotechnology 18(5), 958-963.