Evaluation of photoperiod and thermosensitive genic male sterile lines for hybrid rice seeds production in Kenya

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Evaluation of photoperiod and thermosensitive genic male sterile lines for hybrid rice seeds production in Kenya

Kanya James Ireri, Njiruh Paul Nthakanio, Kimani John Munji, Wajogu Rapheal Kinyanjui, Kariuki Simon Njau
Int. J. Agron. Agri. Res.3( 2), 21-39, February 2013.
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Photo–thermo-sensitive genic male sterile (P(T)GMS) rice is a new hybrid rice technology that uses prolonged light length and high temperatures to induce sterility. This technology is being introduced in Kenya but such growth conditions are lacking in the tropics. To overcome this, we grew P(T)GMS under greenhouse conditions where day light length was prolonged to 14 hrs using solar illumination and day and night temperatures were maintained above 36Cº and 24ºC respectively. Sterility of P(T)GMS was determined by the level of abortive pollen and seed set rates. Hybrid seeds were produced by crossing three P(T)GMS lines (V1PGM, V2TGM and V3PGM) as female lines with Basmati 370 and Basmati 217 varieties as pollen donors. Under long and normal day lengths and high temperatures, pollen sterility ranged from 99-100% but no seeds were set in PGMS lines. However, TGMS recorded 3% and 2% seed set under similar conditions. Under natural conditions both PGMS and TGMS reverted to fertility. Agronomic evaluation showed outstanding (P<0.01) performance of hybrids over parents in flag leaf length (V2370; 35.8 cm), panicle exsertion (V2217; 8cm) and shorter flowering time (V3217; 85 days) under greenhouse conditions while flag leaf width (V1370; 1.48 cm) and tillering (100) performed better under natural conditions. Percentage seed set positively correlated with flag leaf related traits and flowering days but negatively correlated with panicle length, panicle exsertion and tillering. We conclude that the P(T)GMS lines are adaptable for hybrid rice seed production in Kenya but there is need to review the P(T)GMS lines’ background so as to minimize incompatibility in hybrids.


Ahmadikhah A, Nasrollanejad S, Alishah O. 2008. Quantitative studies for investigating variation and its effect on heterosis of rice. International Journal of Plant 2, 297–308.

Akhilesh KS, Pawan K, Rahul P, Virupaxagouda P, Manisha D, Vinay S. 2011. Occurrence of Trifid Stigma Morphotype in a Maintainer Line of Rice (Oryza sativa L.), International Journal of Plant Breeding and Gentics 6(4), 252-255.

Ali J, Siddiq EA, Zaman FU, Abraham MJ, Ilyas DA. 1995. Identification and characterization of temperature sensitive genic male sterility sources in rice (Oryza sativa L.). International Journal of Genetics 55(3): 243-259.

Bagal UR, Leebens-Mack JH, Lorenz WW, Dean JF, D. 2012. Phylogenomic analysis of the phenylalanine ammonia lyase gene family in loblolly pine (Pinus taeda L.). BioMed Central Genomics. DOI: 10. 1186/1471-2164-13-S3-S1.

Bardhan RSK, Pateña GF, Vergara BS. 1982. Feasibility of selection for traits associated with cold tolerance in rice under rapid generation advance method. Euphytica 31, 25-31, 1982.

Blake NK, Lanning SP, Martin JM, Sherman JD, Talbert LE. 2006. Relationship of Flag Leaf Characteristics to Economically Important Traits in Two Spring Wheat Crosses. Crop Sciience 47: 491-494.

Dalmacio B, Sitch I, Kush V. 1995. Identification and transfer of new cytoplasmic male sterile source from  Oryza  perennis  into  indica  rice  (O.  sativa). Euphytica 82, 221-225.

Dere S, Yildirim MB. 2006. Inheritance of grain yield per plant, flag leaf width and length in 8 x 8 Diallel cross pollination of bread wheat (T. aestivum L.). Turkish Journal of Agriculture and Forestry 30, 339-345.

Donald CM. 1968. The breeding of crop ideotypes. Euphytica 17, 385–403.

Dwivedi DK. 2012. Gene Action and Heterosis for Yield and Associated Traits in Indica and Tropical Japonica Crosses of Rice (Oryza sativa L.) Involving Wide Compatibility Gene(s). International Journal of Plant Breeding and Genetics 6(3), 140-150.

Evans LT. 1998. Feeding the Ten Billion: Plants and Population Growth, PP. 5-12. Cambridge University Press, Cambridge, UK.

Falster DS, Reich PB, Ellsworth DS, Wright IJ, Westoby M, Oleksyn J, Lee TD. 2011. Lifetime return on investment increases with leaf lifespan among 10 Australian woodland species. New Phytology 10, 1469-1489.

Hasegawa T. and Horie T. 1996 Rice leaf photosynthesis as a function of nitrogen and crop developmental stage. Crop Science 65, 553-554.

Hittalmani S, Shashidhar HE, Bagali P, Huang N, Sidhu JS, Singh VP, Khush GS. 2002. Molecular mapping of quantitative trait loci for plant growth, yield and yield related traits across three diverse locations in a doubled haploid rice population. Euphytica 125: 207-214.

Ikehashi H, Araki H. 1986. Genetics of F1 Sterility in Remote Crosses of Rice. In Rice Genetics (ed. IRRI), PP. 119-130. International Rice Research Institute, Manila, Philippines.

International Rice Research Institute (IRRI). 2009. Accelerating Hybrid rice development. In (ed. F. Xie and B. Hardy), PP. 3-8. Metro Manila, DAPO Box 7777, Philippines.

Islam MD, Hasanuzzaman M, Rokonuzzaman M. 2009. Effect of split application of nitrogen fertilizer on morphophysiological parameters of rice genotypes. International Journal of Plant Production 3(1), 1735-6814.

Kanya JI, Hauser TP, Kinyamario JI, Amugune NO. 2012. Hybridization potential between cultivated rice Oryza sativa and African wild rice Oryza longistaminata. International Journal of Agricultural Research 7(6), 291-302.

Kaushal P, Ravi N. 1998. Crossability of wild species of Oryza with O. sativa cvs PR106 and Pusa Basmati 1 for transfer of bacterial leaf blight resistance through interspecific hybridization. Agricultural Science 130, 423-431.

Kende H, van der Knaap E, Cho HT. 1998. Deep water rice: a model plant to study stem elongation. Plant Physiology 118, 1105–10.

Kikuchi F, Ikehashi H. 1984. Semi dwarfing genes of high-yielding rice varieties in Japan. Rice Genetics Newsletter 1: 93-94.

Ku C, Kim B, Chung S. 2001. Cytological observation of two environmental genic male sterile lines of rice. Molecular Cell 12(3), 403-406.

Kush GS. 1994. Increasing the genetic yield potential of rice: prospects and approaches. International Rice commission Newsletter 43, 1-7.

Lin I, Kawashima Y. 1992. Segregation distortion via male gametes in hybrids between Indica and Japonica or wide compatibility varieties of rice (Oryza sativa L.). Theoretical and Applied Genetics 84, 812-818.

Lopez MT. 2000. Development of TGMS lines for developing two-line hybrids for the tropics. Euphytica 114, 211–215.

Ma A, Nawab NN, Abbas A, Zulkiffal M, Sajjad M. 2009. Evaluation of selection criteria in Cicer arietinum L. using correlation coefficients and path analysis. Australian Journal of Crop Science 3, 65-70.

Ministry of Agriculture. 2010. The ministry of agriculture at a glance. Office of the Permanent secretary, Kenya.

Muhammad A, Khan AS, Khan SHU, Ahmad R. 2012. Association of various morphological traits with yield and genetic divergence in rice (Oryza sativa). International Journal of Agriculture and Biology 1814–9596.

Naokuni E, Izawa T. 2011. Flowering time genes heading date 1 and early heading date 1 together control panicle development in rice. Plant Cell Physiology 52: 1083-1094.

Nguyen NV. 2010. Ensuring food security in the 21st century with hybrid rice: issues and challenges. Accelerating hybrid rice development, PP. 9-24. IRRI-Manila, Philippines.

Njiruh PN, Xue Q. 2011. Programmed cell death-like behavior in photoperiod sensitive genic male sterile (PGMS) rice. African Journal of Biotechnology 10(16), 3027-3034.

Oka HI. 1974. Analysis of genes controlling F1 sterility in rice by the use of isogenic line. Genetics 77, 521-534.

Reddy AS. 2007. Alternative splicing of pre-messenger RNAs in plants in the genomic era. Annual review of Plant Biology 58, 267–294.

Santhosh P, Sudheer KS, Ahmed MI. 2009. Characterization of temperature sensitive genic male sterile (TGMS) lines in rice (Oryza sativa L.). Oryza 44, 1-6.

Sasahara TT, Kayaba T, Tsunoda S. 1992. A new strategy for increasing plant productivity and yield in rice. IRC Newsletter 41, 1-6.

Schnable PS, Stinard P, Wen TJ, Heinen S, Weber D, Zhang L, et al. 1994. The genetics of cuticular wax biosynthesis. Maydica 39, 279-287.

Schnable PS, Wise RP. 1998. The molecular basis of cytoplasmic male sterility and fertility restoration. Trends in Plant Science 3, 175–180.

Shanti L, George ML, Vera Cruz CM, Bernardo MA, Nelson RJ, Heung H. 2001. Identification of resistance genes effective against rice bacterial blight pathogen in eastern India. Plant Diseases 85, 506-512.

Shi MS. 1981. Preliminary research report on breeding and utilization of the natural two-uses line in late japonica rice. Scientia Agricola 7, 1-3.

Shi MS. 1985. The discovery and study of the photosensitive recessive male-sterile (Oryza sativa L. subsp. japonica). Scientia Agriculturae Sinica 19, 44-48.

Shi MS, Deng JY. 1986. The Discovery, Determination and Utilization of the Hubei Photosensitive Genic Male-sterile Rice (Oryza sativa subsp. japonica). Acta Genetica Sinica 13(2), 107-112.

Tari DDB, Gazanchian A, Pirdashti HA, Nasiri M. 2009. Flag leaf Morph-physiological Response to Different Agronomical Treatments in a Promising Line of Rice (Oryza sativa L.). American-Eurasian Journal of Agriculture and Environmental science 5: 403-408.

Tua S, Luana L, Liua Y, Longa W, Konga F, Hea T. et al. 2007. Production and heterosis analysis of rice autotetraploid hybrids. Crop Science 47: 2356-2363.

Virmani SS, Viraktamath BC, Lopez MT. 1997. Nucleus and breeder seed production of thermosensitive genic male sterile lines. International Rice Research Newsletter 22(3), 26-27.

Virmani SS. 1992. Transfer and induction of thermosensitive genic male sterile mutant in indica rice. In: Proceedings of the second international symposium on hybrid Rice held on 21-25 April 1992. International Rice Research Institute, Manila (Philippines).

Virmani SS, Kumar I. 2004. Development and use of hybrid rice technology to increase rice productivity in the tropics. International Rice Research Newsletter 29(1), 10-19.

Wang F, Hengn F, Zhang G. 2007. Difference in grain yield and quality among tillers in rice genotypes differing in tillering capacity. Rice Science 14, 135-140.

Wang YG, Xing QH, Deng QY, Liang FS, Yuan LP, Weng ML, Wang B. 2003. Fine mapping of the rice thermo-sensitive genic male-sterile gene tms5. Theoretical Applied Genetics 107, 917– 921.

WARDA. 1999. Crossing African and Asian rice species. Report of advances in rice research. West African Rice Development Association, PP. 13-36. Bouake, Cote d’Ivoire.

Weider LJ, Frisch D, Hebert PD. 2010. Long-term changes in metapopulation genetic structure: a quarter-century retrospective study on low-Arctic rock pool Daphnia. Proceedings of the Royal Society 277, 139–146.

Xiao H, Wang W. 2008. Elongation of the Uppermost Internode for Changxuan 3S, a Thermo-Sensitive Genic Male Sterile Rice Line. Rice Science 15, 2009-214.

Xue E, Zhang L, McCouch Y, Earl ED. 1999. Production and testing of plants regenerated from proplasts of photoperiod sensitive genic male sterile rice (Oryza sativa L.). Euphytica 205, 167-172.

Yang Z, Sun X, Wang S, Zhang Q. 2003. Genetic and physical mapping of a new gene for bacterial blight resistance in rice. Theoretical Applied Genetics 106, 1467–1472.

Yang Z, Xu C, Wang Y. 1996. Theories and methods of rice breeding for maximum yield. Acta Agronomica Sinica 22(3), 297-304.

Yuan SC, Zhang ZG, He HH, Zen HL, Lu KY, Lian JH, Wang BX. 1993. Review and interpretation of two photoperiod-reactions in photoperiod-sensitive genic male-sterile rice. Crop Science 33(4), 651-660.

Zafar N, Aziz S, Masod S. 2004. Phenotypic divergence for agro-morphological traits among landrace genotypes of rice (Oryza sativa L.) from Pakistan. International Journal of Agriculture and Biology 2, 335–339.

Zhang Q, Liu KD, Yang GP. 1997. Molecular marker diversity and hybrid sterility in indica-japonica rice crosses. Theoretical Applied Genetics 95, 112-118.

Zhang QF, Zhen BZ, Dai XK, Mei MH, SaghaMaroof MA, Li ZB. 1994. Using bulked extremes and recessive class to map genes for photoperiod-sensitive genic male sterility in rice. Proceedings of the National Academy of Sciences 91, 8675- 8678.