Evaluation of progenies from crosses between Bt and non-transgenic sweet potato (Ipomoea batatas)

Paper Details

Research Paper 01/03/2013
Views (257) Download (8)
current_issue_feature_image
publication_file

Evaluation of progenies from crosses between Bt and non-transgenic sweet potato (Ipomoea batatas)

R.J. Rukarwa, S.B. Mukasa, G. Ssemakula
Int. J. Agron. Agri. Res.3( 3), 28-37, March 2013.
Certificate: IJAAR 2013 [Generate Certificate]

Abstract

In this study, transgenic event, CIP410008.7 was used as donor parent of cry7Aa1 gene and a commercial cultivar, New Kawogo, was used as the recipient parent. A total 53 progenies were obtained and evaluated. The characters evaluated were vine vigour, vine internode length, vine length, foliage weight, root skin and flesh colour, carotenoid content, number of storage roots and weevil resistance. Significant variation in vine vigour, vine length, number of roots per plant and carotenoid content (p<0.05) was observed in the F1 progeny. However, no significant differences were observed for foliage weight and vine internode length. The existence of continuous variation of the measured traits indicated the quantitative nature of most sweetpotato traits. The progenies were also analysed for the presence of cry7Aa1 using PCR. Expected 608bp bands were amplified in progenies that contained cry7Aa1. Chi-square test showed a 1:1 segregation ratio. There was no significant difference in production of roots between the transgenic and non transgenic segregants, showing that the transgenic plants were not affected by the presence of cry7Aa1. In insect bioassays, there were significant differences in the mean development period of the weevil (p=0.03), total number emerged (p<0.001) and susceptibility index (P<0.001) between the transgenic progeny and control sweetpotato roots.

VIEWS 2

Ashikari  M,  Matsuoka  M,  Datta  SK.  2004. Transgenic rice plants. In: Curtis IS, ed. Transgenic Crops of the World: essential protocols. MA, USA: Kluwer Academic publishers, p. 3-18.

Blanche SB, Myers GO, Zumba JZ, Caldwell D, Hayes J. 2006. Stability comparisons between conventional and near isogenic transgenic cotton cultivars. Journal of Cotton Science 10, 17-28.

Burgos G, Carpio R, Sanchez C, Sosa P, Porras E, Espinoza J, Grüneberg W. 2009. Guide for using the Royal Horticultural Society (RHS) colour chart for selecting for high β-carotene sweetpotato. Lima, Peru: CIP/harvest Plus, p. 30.

Dobie P. 1977. The contribution of the tropical stored products centre to the study of insect resistance in stored maize. Tropical Stored Products Information 34, 7-22.

Fuglie KO. 2007. Priorities for sweetpotato research in developing Countries: Results of a survey. HortScience 42, 1200-1206.

Hussein HM, Habuštová O, Sehnal F. 2005. Beetle-specific Bacillus thuringiensis Cry3Aa toxin reduces larval growth and curbs reproduction in Spodoptera littoralis (Boisd.). Pest Management Science 61, 1186-1192, http://dx.doi.org/10.1002/ps.1112

Kapinga RE, Carey EE. 2003. Present status of sweetpotato breeding for eastern and southern Africa. In: Rees D, Oirschot Q and Kapinga R, ed. Sweetpotato post-harvest assessment: experiences from East Africa. Chatham, UK: Natural Resources Institute, p. 3-8.

Kreuze KF, Valkonen JPT, Ghislain M. 2009. Genetic Engineering. In: Loebenstein G, Thottappilly G, ed. The Sweetpotato. New York: Springer-Verlag New York Inc, p. 41-43.

Luo HR, Santa Maria M, Benavides J, Zhang DP, Zhang YZ, Ghislain M. 2006. Rapid genetic transformation of sweetpotato (Ipomoea batatas (L.) Lam) via organogenesis. African Journal of Biotechnology 5, 1851-1857.

Lynch PT, Jones J, Blackhall NW, Davey MR, Power JB, Cocking FC, Nelsan MR, Bigelow DM, Orum TV, Orth CE, Schuh W. 1995. The phenotypic characterization of R2 generation transgenic rice plants under field and glasshouse conditions. Euphytica 85, 395–401, http://dx.doi.org/10.1007/BF00023972

Mishra MK, Slater A. 2012. Recent Advances in the Genetic Transformation of Coffee. Biotechnology Research International. p. 17, http://dx.doi.org/10.1155/2012/580857

Mwanga ROM, Odongo B, Ocitti p’Obwoya C, Gibson RW, Smit NEJM, Carey EE. 2001. Release  of  five  sweet  potato  cultivars  in  Uganda. HortScience 36, 385-386.

Osborn TC, Pires JC, Birchler JA, Auger DL, Chen ZJ, Se LH, Comai L, Madlung A, Doerge RW, Colot V, Martienssen RA. 2003. Understanding mechanisms of novel gene expression in polyploids. Trends in genetics 19, 141-47, http://dx.doi.org/10.1016/S0168-9525(03)00015-5

Paul A. 2003. Production and characterization of transgenic Brassica oleraceae carrying endotoxin genes of Bacillus thuringiensis. Biological Control 48, 611–636.

Rees  D,  Van  Oirschot  Q,  Kapinga  R.  2003. Sweetpotato Post–Harvest Assessment: Experiences from East Africa. Chatham, UK: Natural Resources Institute, p. 129.

Smit NEJM. 1997. Integrated Pest Management for sweetpotato in Eastern Africa. PhD Thesis, Agricultural University Wageningen, The Netherlands, p. 151.

Stacey J, Isaac PG, Rapley R. 2000. Isolation and purification of DNA from plants. In: The Nucleic Acid Protocols Handbook. New York: Humana Press Inc, p. 13-16

Stathers TE,  Rees D,  Nyango  A,  Kiozya H, Mbilinyi L, Jeremiah S, Kabi S, Smit N. 2003. Sweetpotato infestation by Cylas spp. in East Africa: II. Investigating the role of root characteristics. International Journal of Pest Management 49,141-46, http://dx.doi.org/10.1080/0967087021000043094

Stevenson PC, Muyinza H, Hall DR, Porter EA, Farman D, Talwana H, Mwanga ROM. 2009. Chemical basis for resistance in sweetpotato Ipomoea batatas to the sweetpotato weevil Cylas puncticollis. Pure Applied Chemistry 81, 141-151. doi: 10.1351/PAC-CON-08-02-10

Takahata Y, Noda T, Nagata T. 1993. HPLC determination of β-carotene of sweetpotato cultivars and its relationship with colour values. Japan Journal of Breeding 43, 421-427.

Visarada KBRS, Meena K, Aruna C, Srujana S, Saikishore N, Seetharama N. 2009. Transgenic Breeding: Perspectives and Prospects. Crop Science 49, 1555-1563, http://dx.doi.org/10.2135/cropsci2008.10.0581