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Research Paper | October 1, 2013

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Evaluation of rice varieties using proteomic approach

Shazia Dilber, Khushi Muhammad, Inamullah, Muhammad Shahid Nadeem , Fida Muhammad Abbasi, Nazia Akbar, Ikram Muhammad, Habib Ahmad

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Int. J. Biosci.3(10), 8-14, October 2013

DOI: http://dx.doi.org/10.12692/ijb/3.10.8-14

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Abstract

In present study, proteomics approach was used to evaluate three varieties i.e. Bas-385, Indica and KS-282 of rice. Total crude protein was isolated from root, endosperm, embryo and leaf sheath and leaf blade of these varieties and it was separated by SDS-PAGE. The protein bands were scored and used to compare the rice varieties. Seven protein bands in endosperm, six in embryo, seven in roots, six in leaf sheath and seven in leaf blade were recorded conserved proteins during this study. Three protein bands of 25, 30 and 160KDa were detected in the endosperm protein of Bas-385 and JP-5, sharing a common genetics of Basmati and non Basmati. A band of 17KDa was detected in the embryo of Bas-385 while absent in KS-282 and JP-5. Three protein bands i.e. 27, 90 and 120KD were detected in the leaf blade of Bas-385. A band of 45KDa was detected in the leaf blade of JP-5 and absent in KS-282 and Bas-385. A band of 61KDa and three bands of 25, 85 and 175KDa were detected in the embryo and leaf sheath of JP-5 respectively and absent in KS-282 and Bas-385. It is indicating that these proteins could determine the Japonica characters in JP-5. Two bands of 35KDa and 40KD were recorded in endosperm and root of KS-282 respectively while absent in Bas-385 and JP-5. This study explored that there are proteins that are specific for Basmati rice and non Basmati rice or Indica and Japonica rice and could be used to identify rice cultivars.

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Evaluation of rice varieties using proteomic approach

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