Evaluation of snake bean (Vigna sesquipedalis) through agro-morphological and SDS-PsAGE base markers
Paper Details
Evaluation of snake bean (Vigna sesquipedalis) through agro-morphological and SDS-PsAGE base markers
Abstract
The present panel of work was conducted to investigate intra-specific genetic variability among Snake bean landraces. Genetic variation is considered as a basic feature to plants breeding and makes it viable to choose plants with appropriate traits. The methods to study genetic variation are morphological traits and SDS-PAGE. For this purpose, seeds of 40 landraces were collected. The morphometric parameters include both qualitative and quantitative traits. In 4 qualitative parameters significant level of variation was observed in seed shape, seed color and pod pigmentation except legume colour. For 11 quantitative traits descriptive statistics analysis done in which 100 seed weight show maximum coefficient of variance CV (0.37%) followed by pod length (0.32%), While minimum coefficient of variance showed by internode length (0.10%) followed by plant height 0.16% and seed length (0.18%). In comparative correlation, the plant height show highly positive correlation with plant biomass (.705**), seed per pod (.464**) and pod length (.362*), while highly negative correlation obtained with leaf length (-.335*).The protein profile of 40 snake bean landraces tested through SDS-PAGE shown 15 reproducible bands. High level of variation was observed in band B14 (0.93%), followed by B11 (0.80%), B15 (0.78%) B12 (0.75%), B13 (0.75%), B1 (0.65%), B5 (0.50%) and B8 (0.50%). Similarly low level of variation was noted in B3 (0.15%) followed by B4 (0.33%), B10 (0.38%), B6 (0.40%), B2 (0.45%), B7 (0.48%) and B9 (0.50%). The entire bands show polymorphism. All the traits show outmost level of divergence which is advantageous for further improvement of best variety.
Akinjogunla OJ, Taiwo MA, Kareem KT. 2008. Immunological and molecular diagonstic method for detection of viruses infecting cowpea (Vigna unguiculata). African Journal of Biotechnology 7, 2099-2103.
Andrew PS. 2002. Conversation Biology (1Edn) Cambridge University Press, Cambridge 345 p.
Amaliotis D, Therios I, Karatissiou M. 2004. Effect of nitrogen fertilizer on growth, leaf nutrient concentration and photosynthesis in three peach cultivars. ISHS, Acta Hort. 499, 36-42.
Anonymous. 2002. Horticultural export development component: IDA funded. Dhaka, Bangladesh 1-2.
Arshad M, Naazarali, Abdulghafoor. 2006. Character correlation and path coefficient in soybean Glycine max (L.) Merrill. Pakistan Journal of Botany 38(1), 121-130.
Asaf K, Zabta KS, Ajmal I, Syed FW, Nausheen, Mohammad N. 2016. Conventional breeding enhances genetic diversity in Pisum sativum (L.) Detected through sds-page. Pakistan Journal of Botany 48(6), 2433-2438,
Bashir M, Ahmad Z, Ghafoor A. 2002. Cowpea germplasm evolution for virus resistance under greenhouse condition. Asian Journal of Plant Science 1, 585-587.
Benchasri S, Bairman C, Nualsri C. 2012. Investigation of cowpea and yard long bean for Resistance to Bean Aphids (Aphis craccivora Koch) in southern part of Thailand. Proceeding of the international conference on Agricultural and Animal Science 22, 119-121.
Benchasri S, Bairman C, Nualsri C. 2011. Evalution of yard long bean and cowpea for resistance to Aphis craccivoraa Koch in southern part of Thailand. The journal of Animal and Plant Science 22(4).
Branca F, La Malfag G. 2008. Traditional Vegetable of Silicy. Chronica Horticulturae 48, 20-25.
Duangjai Chanapan, Sorapong Benchasri, Sakunkansimla. 2017. investigation of inorganic and organic agricultural system for vignaspp. production in Thailand, department of plant science, Faculty of Technology and Community Development, Thaksin University, Pa Phayom, Phatthaling Thailand P.O.93210 2D.Austraian Journal of Crop science.
FAQ. 2013. Yard long bean (Vigna unguiculata (L) Walpers cv-gr. SesquipedalisE.Westphal ]aource; yard long bean –NIAS Genebank.).
Franche C, Lindstrom K, Elmerich C. 2009. Nitrogen-fixing bacteria associate with leguminous and non-leguminous plants. Plant Soil 321, 35-59.
Fuller D, Harvey EL. 2006. The archaeo botany of Indian pulses: identification, Processing and evidence for cultivation. Environmental Archaeology 11: 219-246.
Ghafoor A, Ahmad Z, Afzal M. 2005. Use of SDS-PAGE markkers for determining quantitative traits loci in blackgram [Vigna mungo (L.) Hepper] germplasm. Pak. J. Bot., 37(2), 263-269.
Hammer K. 1984. Das Domestikations syndrom. Genetic resources and Crops Evolution 32, 11-34.
Hansen V. 2013. Snake (yard long bean) bean, black book cooking, available online: www.blackbpplcooking.com/snakebeanoryardlongbean.
Hassan A, Khaliq Nausheen T, Jan A, Ghafoor M, Khan, Nisar M. 2016. Genetic polymorphism in Lens culinaris collected from Malakand Division Khyber Pakhtunkhwa, Pakistan. J. of Bio. AndEnvl. Sci. 8(2), 53-60.
Nursu’aidah H, Motior MR, Nazia AM, Islam MA. 2014. Geowth and photosynthetic response of long bean (Vigna unguiculata) and mung bean (vigna radiate) response to fertilizer, The Journal of Animal & Plant Science 24(2), 2014, page, 573-578.
Hugqe AM, Hossain MK, Alam N, Hasaanuzzaman M, Biswas BK. 2012. Genetic divergence in yard long bean (Vigna unguiculata (L.) Walp. ssp. Sesquipedalid Verdc.). Bangladesh Journal of Botany, 41(1).
Izawa T, Konishi S, Shomura A, Yano M. 2009. DNA changes tell us about rice domestication. Current Opinion in Plant Biology 12, 185-192.
Karistsapol Nooprom Quanchit Santipracha 2015. Effect of varieties on growth and yield of yard long bean under songkhla conditions, Southern Thailand; Modern applied Science 9(13), 2015.
Karp A, Edwards K. 1997. DNA markers: a globl overview. In DNA Markers, Protocols, Application and Overview, p1-13 (Eds; Caetano Anolles, G. and P.M. Gresshoff). New York: Wiley-liss, Inc.
Kishitij Khatri, Guodong Liu, Qingren Wang, Yuncong Li, David Dinkins, Bonnie Wells 2015. Long Bean –an Asian Vegetable Emerhing in Florida. UF/IFAS Extension HS1268.
Ladizinsky G, Hymowitz T. 1979. Seed protein electrophoresis in taxonomic and evolutionary studies. Theoretical and Applied Genetics 54, 145-151.
Lawrence JH, Moore LM. 2012. Plant Guide: Yardlong Bean. USDA Natural Resources Conservation Service. Accessed October 1, 2015. http//plants.Usds.gov/plantguide/pdf/pg-viuns2.
Litty Varghese Dr. Celine VA. 2015. Department of Olericulture, College of Agriculture, Vellayanikerala. Evalution of yard long bean (Vigna unguiculata subsp. Sesquipedalis (L.) Verdcourt genotypes for yield and quality character under polyhouse. International Journal of Applied and pure science and Agriculture.
Mohiuddin M, Hossain MM, Rahman AKMM, palash MS. 2009. Socioeconomic study of insecticide use on vegetable cultivation at farm level in chittagong region. Journal of the Bangladesh Agricultural University 7(2), 343-350.
Mustapha Y, Singh BB. 2008. Inheritance of pod colour in cowpea (Vigna unguiculata (L.) Walp). Science World Journal 3, 39-42.
Nagalakshmi RM, Kumari RU, Kumari RU, Boranayaka MB. 2010. Assessment of Genetic diversity in cowpea (Vigna unguiculata). Electronic Journal of Breeding 1, 453-461.
Newman JM. 2006. Long beans are twins. Flavor and fortune, dedicated to the art and science of Chinese cuisine. Vegetables and vegetarian foods 13, 25-28. Lakshmi.
Nisar M, Ghafoor A, Ahmad H, Rashid KM, Qureshi AS, Ali H. 2008. Evaluation of genetic diversity of pea germplasm through phenotypic traits analysis. Pak. J. Bot., 40(3).
Ofri K, Klogo PY. 2005. Optimum time for harvesting yard long bean (Vigna unguiculata) for high yield and quantity of pods and seeds. Journal of Agriculture and Social science.
Power your diet. 2013. Yard long bean nutrition facts, 2009-13.
Purugganan MD, Fuller DQ. 2009. The nature of selection during plant domestication. Nature 457, 843-848.
Rachie KO. 1985. Introduction. In: Singh, S. R., Rachie, K., O, John, W. a. s. (Eds), cowpea Research, Production and Utilization, Chichester UK.
Rahman MM, Munsur MAZ. 2009. Genetic divergence analysis of lime. Journal of Bangladesh Agriculture University 7, 33-37.
Rambabu E, Ravinder Reddy K, Kamala V, Saidaiah P, Pandravada SR. 2016. International Journal of Science and Nature. I.J.S.N., 7(2), 2016 344-34. ISSN 2229–6441.
Ramya V. 2011. Detection of viable urediniospores, morphological Characterization in resistant and susceptible genotypes, and germ Tube anastomosis of phakopsorapachyrhizi.
Rao NK, Hanson J, Dulloo ME, Ghosh K, Nowell D, Larinde M. 2006. Manual of seed handling in genebanks. In Handbooks for Genebanks No. 8. Biodiversity International, Rome, Italy. 147, p.
Sarutayophat T, Nualsri C, Santipracha Q. 2007. Characterization and genetic relatedness among 37 yard long bean and cowpea accession based on morphological characters and RAPD analysis Songklanakarin Journal of Science and Technology, 29(3). 120-129
Teerawat Sarutayophat, Charssari Nualsri, Quanchit Santipracha Vinich Saereeprasert. 2007. Characterization and genetic relatedness among 37 yardlong bean and cowpea accessions based on morphological characters and RAPD analysis.
Timko MP, Ehlesr JD, Roberts PA. 2007. Cowpea. In: kole, C. (Ed0, Genome Mapping and Molecular Breeding in Plants, Volume 3 Pulses, Sugar and Tuber Crops. Springer-Verlag berlin Heidelberg, p 49-67.
Tucker M. 2004. Primary nutrients and plant growth-in: Essential plant nutrients (Scribd, Ed.). North Carolina Department of Agriculture.
USDA-ARS. 2012. United State Department of Agriculture (USDA). Plant Guide: yard long bean Vigna unguiculata (L.) Walp. Ssp. Sesquipedalis (L.) Verdc.
Vasagam KPK, Rajkumar M. 2011. Beneficial influences of germination and subsequent autoclaving of grain legumes on proximate composition, antinutritional factors and apparent digestibility in black tiger shrimp, Penaenus monodon fabricus. Aquacult. Nutr 17(2), 188195.
Verdcourt B. 1970. Studies in the Leguminosae –Papilionoideae for the flora of Tropical East Africa.
Karishma Gul, Murad Ali, Arshad Khan, Khwaja M Khan, Sundas Wisal, Waqar Khan, Mohammad Nisar (2019), Evaluation of snake bean (Vigna sesquipedalis) through agro-morphological and SDS-PsAGE base markers; JBES, V14, N1, January, P34-45
https://innspub.net/evaluation-of-snake-bean-vigna-sesquipedalis-through-agro-morphological-and-sds-psage-base-markers/
Copyright © 2019
By Authors and International
Network for Natural Sciences
(INNSPUB) https://innspub.net
This article is published under the terms of the
Creative Commons Attribution License 4.0