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Evaluation of the antimicrobial activities of different solvent extracts from the bark of Acacia Stenophylla A. CUNN. EX. BENTH.

Research Paper | November 1, 2020

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Dawood Shah, Arshad Iqbal

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Int. J. Biosci.17( 5), 184-194, November 2020

DOI: http://dx.doi.org/10.12692/ijb/17.5.184-194


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The present study was conducted to evaluate the in-vitro antimicrobial activities of crude extracts derived from the bark of Acacia stenophylla by different organic solvents such as N-Hexane, Chloroform, Ethyl acetate, and N-Butanol. These solvent extracted samples were applied against three Gram-positive bacteria including Bacillus atrophaus, Bacillus subtilis, and Staphylococcus aureus, as well as four Gram-negative bacteria that comprised Klebsiella pneumonia, Pseudomonas aeruginosa, Salmonella typhi, and Escherichia coli. Additionally, the antimicrobial activities of these samples were also tested against one fungal strain i.e. Candida albicans. The antimicrobial potentials were measured by using the Well diffusion method in three different doses of 1, 2, and 3 mg, respectively. The antibiotic ciprofloxacin, anti-fungal clotrimazole, and DMSO were used as positive and negative control. Our results suggest that Salmonella typhi was a highly sensitive bacterial strain followed by Staphylococcus aureus showing 87.34% and 72.41% ZI, respectively.  Similarly, Bacillus atrophaus was found the most resistant bacterium tested. N-hexane was highly potent against Salmonella typhi that indicates that biologically active substances against S.typhi­ are non-polar. N-Hexane fraction showed maximum inhibitory potential against gram-positive bacteria as compared to other solvent fractions while crude methanol demonstrated maximum anti-microbial strength against Gram-positive, Gram-negative bacteria as well as fungal strain. This specifies the broad-spectrum antibacterial and antifungal potential of the crude methanol. Our study verified that crude methanol and its different solvent fractions extracted from the bark of A. stenophylla have promising antimicrobials that can be used against various bacterial and fungal diseases. Furthermore, a study is needed for the optimization of antimicrobials of the plant for commercial utilization.


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