Evaluation of the effectiveness of Metarhizium anisopliae on Callosobruchus maculatus Fab. (Coleoptera: Bruchidae) and Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae) in laboratory
Paper Details
Evaluation of the effectiveness of Metarhizium anisopliae on Callosobruchus maculatus Fab. (Coleoptera: Bruchidae) and Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae) in laboratory
Abstract
Callosobruchus maculatus and Sitophilus zeamais are major storage insect pests of cowpea, Kersting’s groundnut and maize in tropical Africa. These pests can cause post-harvest losses of up to 100% in a few months. The most common suppression method is the use of pesticides, with unfortunately important hazards and side effects. This study, evaluated the effectiveness of four isolates of Metarhizium anisopliae (ma351, ma356, ma357 and ma358) on C. maculatus and S. Zeamais in stored grains of cowpea, Kersting’s groundnut and Maize, respectively. The pathogenicity of the four isolates was assessed and two of them (ma356, ma357) were the most effective. To study the virulence of these two isolates, four doses were used 0, 108, 109, 1010 conidia/100 grains. Results from the pathogenicity test indicated that all studied isolates were pathogenic to insects at a dose of 109 conidies/100 grains equivalent to 0.002 g/100 grains powder form. In the presence of isolates, the lifespan of treated pests was reduced compared to the control insect pests. The effectiveness of the isolates was significantly dose-dependent for all parameters evaluated except for imagos hatching rate of C. maculatus on cowpeas and seed weight loss in maize. The dose of 1010 conidia/100grains significantly reduced longevity of S. zeamais whereas with the dose of 109 conidia/100 grains reduction was already significant on C. maculatus for all parameters. This study built from experiments and speculated on how the production of entomophagenic fungi at large scale would be an alternative to the use of chemical pesticides in controlling population of C. maculatus and S. zemaïs in stored products and therein significantly reduce the overall post-harvest losses.
Adégbola PY, Arouna A, Ahoyo NRA. 2011. Acceptabilité des structures améliorées de stockage. Bulletin de la Recherche Agronomique du Bénin – Numéro spécial 2: Aspects économiques du stockage et de la conservation du maïs au Sud-Bénin -Septembre 2011.
Andersen SO. 1979. Biochemistry of insect cuticle. Annual Review of Entomolomology 24, 29-61.
Batta YA. 2005. Control of lesser grain borer (Rhyzopertha dominica Fab. Coleoptera: Bostrychidae) by treatements with residual formulations of Metarhizium anisopliae (Metsh) Sorokin. Journal of Stored Products Research 41, 221-229.
Béhanzin E. 1986. Importance de la lentille de terre (Macrotyloma geocarpum (Harms) dans l’alimentation béninoise: Etude du cas de la ville de Cotonou.
Benhalima H, Chaudhry MQ, Mills KA, Price NR. 2004. Phosphine resistance in store-product insects collected from various grain storage facility in Morocco. Journal of Stored Products Research 40, 241-249.
Douro Kpindou OK, Djegui DA, Glitho A, Tamò M. 2012. Réponse des stades larvaires de Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) à l’application de champignons entomopathogènes Metarhizium anisopliae et Beauveria bassiana. Biotechnologie Agronomie Société et Environnement 16, 283-293.
Gansou G, Yabi M, Kiki E. 2000. Etude des modes de «vente à terme» et de «vente précoce» des récoltes du maïs par les producteurs. PADSA/DANIDA. 43 p.
Gueye MT, Seck D, Wathelet JP, LognayG. 2011. Lutte contre les ravageurs des stocks de céréales et de légumineuses au Sénégal et en Afrique occidentale: synthèse bibliographique. Biotechnologie Agronomie Société et Environnement 15, 183-194.
Hidalgo E, Moore D, Le Patourel G. 1998. The effect of different formulation of Beauveria bassiana on Sitophilus zeamais in stored maize. Journal of Stored Product Research 34, 171-179.
Inglis GD, Goettel MS, Butt TM, Strasser H. 2001. Use of hyphomycetous fungi for managing insect pests. In: Butt TM, Jackson CW, Magan N (eds) Fungi as biocontrol agents: progress, problems and potential. CABI Publishing, Wallingford, UK, 23–55.
Kassa A, Zimmermarm G, Stephan D, Vidal S. 2002. Susceptibility of Sitophilus zeamais (Motsch) (Coleoptera: Curcilionidae) and Prostephanus truncatus (Hom) (Coleoptera: Brotrichidae) to entomopathogenic fungi from Ethiopia. Biocontrol Science Technologie 12, 727-736.
Ngamo LST, Hance T. 2007. Diversité des ravageurs des denrées et méthodes alternatives de lutte en milieu tropical. Tropicultura 25, 215-220.
Ouakid ML, Farine JP, Soltani N. 2005. Evaluation de l’activité entomopathogène d’une souche locale du champignon Metarhizium anisopliae sur les larves de Lymantria dispar. IOBC/Wprs Bulletin 28(8), 185-191.
PADSA. (Programme d’Appui au Développement du Secteur Agricole). 2000. Evaluation à mi-parcours du PADSA. Rapport final. PADSA/DANIDA, Cotonou, Bénin, 80 p.
Regnault-Roger C. 2002. De nouveaux phyto-insecticides pour le troisième millénaire? In : Philogène B.J.R, Regnault-Roger C. & Vincent C., coord. Biopesticides d’origine végétale. Paris : Lavoisier-Éditions Tec & Doc, 19-39.
Pronaf. 2002. Développement, diffusion et adoption participatifs des technologies de niébé pour la réduction de pauvreté et le bien-être durable dans les ménages ruraux des zones semi-arides en Afrique de l’Ouest, 1-6.
Salim M. 2011. Evaluation de l’effet insecticide et de la persistance des huiles essentielles de « callistemon viminalis » G Don, de « xylopia ethiopica » Dunal et « lippia chevalierie » Moldenke sur « Callosobruchus maculatus » Fabricius et « Sitophilus zeamais » Motchulsky, principaux ravageurs des stocks de niébé et du maïs. Master Thesis, biologie animale, Université Cheikh AntaDiop de Dakar, Sénégal.
Sankara F, Dabire LCB, Dugravot S, Cortesero AM, Sanon A. 2012. Capacités de discrimination des femelles de quatres souches de C. maculatus Fab. (Coleoptera: Chrysomelidae, Bruchidae) pour la localisation de l’hôte et la ponte aux dépens d’hôtes secondaires. International Journal of Biological and chemical Sciences 6, 1303-1315.
SAS Institute Inc. 2003. SAS® 9.1 2003. Qualification Tools User’s Guide. Cary, NC, USA: SAS Institute Inc.
Yallou C. 1994. Production et valorisation du maïs à l’échelon villageois en Afrique de l’Ouest: actes du séminaire “Mas prospère”, 25-28 janvier 1994, Cotonou, Bénin. CIRAD-SAR, UNB. 1995. Montpellier: CIRAD-SAR, 304 p. (Colloques: CIRAD) ISBN 2-87614-206-6.
Marilyse Kokoye, Ouorou Kobi Douro-Kpindou, Martine Zandjanakou Tachin, Aimé Hippolyte Bokonon-Ganta (2016), Evaluation of the effectiveness of Metarhizium anisopliae on Callosobruchus maculatus Fab. (Coleoptera: Bruchidae) and Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae) in laboratory; IJAAR, V9, N6, December, P51-64
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