Int. J. Biosci.2(12), 139-145, December 2012
The effect of cassava mill effluent on the microbial populations and physicochemical parameters of the soil at various depths was studied. The result revealed the bacterial isolates as Proteus mirabilis, Proteus vulgaris, Bacillus sp, Staphylococcus saprophyticus, and Klebsiella sp, Streptococcus sp, while the fungal isolates were Aspergillus sp, Penicillium sp, Mucor sp and Rhizopus sp. In addition, total aerobic bacterial count results at P<0.05 revealed no statistical difference between surface (4.2×106 ±2.01 cfu/g) and subsurface counts (3.9×106 ±1.46 cfu/g) while both were significantly higher than the deeper sample counts (2.3×106 ±0.76cfu/g). Total fungal results showed significant difference at P<0.05 between surface (4.2×106±2.38 cfu/g) and subsurface (2.1×106±1.36 cfu/g) sample counts, as well as between surface and deeper sample counts (0.2×106±0.44 cfu/g), while no significant difference was observed between subsurface and deeper sample counts at P>0.05. The pH, cyanogenic glycosides, and C/N ratio all increased with depths while %OC, %N, %OM and ECEC decreased with depth. Cassava mill effluent has negatively affected the microbial populations and physicochemical parameters at various depths.
Akani NP, Nmelo SA, Ihemanadu IN. 2006. Effects of Cassava Mill Effluents on the Microbial Population and Physicochemical Properties of Loamy Soil in Nigeria. 10th Annual Conference of Nigerian Society for Microbiology, Nasarawa State University, Keffi, 10-14th October, 2006.
Akpor OB, Igbinosa OE, Igbinosa OO. 2007. Studies on the Effect of Petroleum Hydrocarbon on the Microbial and Physico- Chemical Characteristics of Soil. African Journal of Biotechnology 6,(16), 1939-1943.
Alesii BA, Fuller WH. 1976. The Mobility of Three Cyanide Forms in Soils. In: Fuller, W. H. (Eds). Residual Management by Land Disposal, Proceedings of Hazardous waste Research Symposium, Tucson, Arizona.
Atlas RM, Bartha R. 1998. Microbial Ecology: Fundamentals and Applications. 4th edn. Benjamin Cummings Publishing Company Inc. London. 300-350.
Cheesbrough M. 2004. District Laboratory Practice in Tropical Countries. (II). University Press, Cambridge.
Cummings SP, Baxter J. 2006. The Current and Future Applications of Microorganisms in the Bioremediation of Cyanide Contamination. Antonie Van Leeuwenhock 90, 1-17.
Ebhoaye JE, Dada JO. 2004. The Effects of Fresh and Aged Cassava Processing Effluent on the Physico-Chemical Properties of Soil. Pakistan Journal of Scientific and Industrial Research 47(1), 13-16.
Ehiagbonare JE, Enabulele SA, Babatunde BB, Adjarhore R. 2009. Effect of Cassava Effluent on Okada Denizens. Scientific Research and Essay 4(4), 310-313.
FAO. 2004. Cassava Industrial Revolution in Nigeria. Codex Alimentarius Commission XII, Supplementary 4, Rome.
Fawole MO, Oso BA. 2000. Laboratory Manual of Microbiology. 2nd ed. Spectrum Books Ltd. Ibadan, 1-115.
Fuller WH. 1977. Movements of Selected Metals, Asbestos, and Cyanide in Soil: Applications to Waste Disposal Problems. EPA-600/2-77-020. U.S. Environmental Protection Agency, Office of Research and Development, Municipal Environmental Research Laboratory, Solid and Hazardous Waste Research Division, Cincinnati, OH.
Fuller WH. 1984. Cyanide in the Environment with Particular Attention to Soil. In: Van Zyl D (Ed). Cyanide and the Environment. Geochemical Engineering Program vol. 1. Colorado State University, Fort Collins, 19-46.
Knowles CJ. 1988. Cyanide Utilization and Degradation by Microorganisms. CIBA Found Symp 140, 3-15.
Knowles CJ, Wyatt J. 1992. Pollution. In: Wyatt, J. (Ed): Microbial Control of Pollution. Cambridge University Press, Cambridge. 113-28.
NRCRI. 2006. Annual Report: Microbiological and Physicochemical Studies of Soil Contaminated with Cassava Processing Effluent at Umudike, Nigeria, 16-19.
Nwaugo VO, Etok CA, Chima GN, Ogbonna CE. 2008b. Impact of Cassava Mill Effluent (CME) on Soil Physicochemical and Microbial Community Structure and Functions. Nigerian Journal of Microbiology 22(1), 1681-1688.
Okafor JO. 2008. Impact of Effluents from Garri Processing Industries on the Environment in Bida, Niger State of Nigeria. Journal of Engineering and Applied Sciences 3(6), 487-490.
Okwu DE, Nwosu PO. 1999. Effects of Cassava Effluents on Soils Around Cassava Processing Mills in Abia State, Nigeria. NRCRI, Umudike, 1-9.
Okwute OL, Isu NR. 2007. Impact Analysis of Palm Oil Mill Effluent on the Aerobic Bacterial Density and Ammonium Oxidizers in a Dumpsite in Anyigba, Kogi State. African Journal of Biotechnology 6(2), 116-119.
Olorunfemi DI, Emoefe EO, Okieimen FE. 2008. Effect of Cassava Processing Effluent on Seedling Height, Biomass and Chlorophyll Content of Some Cereals. Research Journal of Environmental Sciences 2(3), 221-227.
Oti EE. 2002. Acute Toxicity of Cassava Effluent to the African Catfish Fingerlings. J. Aquat. Sc 17, 31-34.
Schlesinger WH, Reynolds JF, Cunningham GL, Huenneke LF, Jarrel WM, Virginia RA, Whitford WG. 1990. Biological Feedback in Global Desertification. Science 247, 1043-1048.
Shakir SH. 1989. Structural Comparison of Soil Macro Invertebrate- Micro Communities in Selected Global Ecosystem with Special Emphasis to Land use. PhD Thesis. State University of New York. 1-7.
Silver-Avalos J, Richmond MG, Nagappan O, Kunz DA. 1990. Degradation of the Metal-Cyano Complex Tetracyanonickel-ate (11) by Cyanide-Utilizing Bacteria. Appl. Environ Microbial56, 3664-3670.
Snedecor GW, Cochran WG. 1987. Statistical Methods. Oxford IBH Publishing Co. Ltd, New Delhi, 20-35.
Towill LE, Drury JS, Whitefield BL, Lewis EB, Galyan EL, Hammon AS. 1978. Review of the Environmental Effects of Pollutants. U.S. Environmental Protection Agency, Cincinnati.
Ubalua AO. 2010. Cyanogenic Glycosides and the Fate of Cyanide in the Soil. Australian Journal of Crop Science 4(4), 223-237.
Uzoije AP, Egwuonwu N, Onunkwo AA. 2011. Distribution of Cyanide in a Cassava –Mill-Effluent Polluted Eutric Tropofluvent Soils of Ohaji Area, South-Eastern Nigeria. Journal of Soil Science and Environmental Management 2(2), 49-57.
Wood BJ. 1977. A Review of Current Methods for Dealing with Palm Oil Mill Effluents. Planter 53, 477-495.