Evaluation of the efficiency of some antioxidant chemical for germination of bean seeds and on the inhibiting growth of the pathogen causing root and stalk rot disease under laboratory

Paper Details

Research Paper 01/03/2018
Views (344) Download (10)
current_issue_feature_image
publication_file

Evaluation of the efficiency of some antioxidant chemical for germination of bean seeds and on the inhibiting growth of the pathogen causing root and stalk rot disease under laboratory

Rekan Hameed Farhan, Hurria Hussien Al-Juboory
J. Bio. Env. Sci.12( 3), 98-109, March 2018.
Certificate: JBES 2018 [Generate Certificate]

Abstract

The study was conducted to isolate and identify the fungi associated with bean root rot, testing their pathogenicity and evaluate the activity of some antioxidants: Ascorbic acid, Benzoic acid, Salicylic acid (SA) and Phylax on bean seed germination and against growth of some pathogenic causes . The results of Isolation and identification showed the presence of nine species which are belong to seven genus of fungi, of these fungi, R. solani was found the more abundant followed by F. solani and M. phaseiolina. While the other fungi recorded lower frequency percentage. The Preliminary test of pathogencity for nine isolates showed a significant increase in the percentage of seed infection compared with 0.00% in control. The results showed that  all of the chemical with its three concentrations (1500, 2500 ,5000 ppm) effects on seed germination percentage and varying degrees at 1 to 24 hours , SA treatments showed significantly increased germination compared with other treatments while there were no significant differences between the soaking the seeds in SA at concentration 2500 ppm and the sedimentation with water for 24 hours at 100% each , and that there was an inverse relation between concentrations and germination. There were significant differences in the inhibition growth of fungi with the entire chemical to varying degrees compared to the fungus alone, with a percentage of inhibition of 0.0%.SA superiority reduced the rate of inhibition of tested fungi relative to other chemicals. It was observed that there was a positive relationship between concentrations and the percentage of inhibition.

VIEWS 12

Ahmed SM. 2010. Effects of Salicylic acid, Ascorbic acid and two fungicides in control of early blight disease and some physiological components of two varieties of potatoes. Journal of Agriculture Research 36(2), 220-236.

Akram NA, Shafiq F,  Ashraf M. 2017. Ascorbic Acid-A Potential Oxidant Scavenger and Its Role in Plant Development and Abiotic Stress Tolerance. Frontiers in Plant Science Journal 8(613), 1-17. http://dx.doi.org/10.3389/fpls.2017.00613

Al- Juboory  HH.   2002.    Effect of Plant Growth Retardants Cultar on Vicia faba  L. Plant Infection by Root Rot Pathogens. Thesis, Plant Protection/Plant Diseases, College of Agriculture, University of Baghdad.

Al-Juboory HH, Juber KS, Hussein SN. 2016. Identification, Pathogenicity and Controlling of the Macrophomina Phaseolina (Tassi) Goid The Causal Agent of The Charcoal Rot Disease On Watermelon. Journal of University Of Duhok, 19(1) (Agri. And Vet. Sciences), Pp 558-564, 2016 (Special Issue) The 2nd Scientific Agricultural Conference (April 26 And 27th 2016).

Al-Mousawy MA, Jaber KS. 2012 . Isolation And Identification Of The Pathogen Causing Root And Stem Rot Disease On Cowpea And Evaluation Of The Azotobacter vinelandii Efficacy For Controlling The Disease Under Labrotary Condetions, The Iraqi Journal of Agricultural Sciences 43(2), (Special Issue), 67-75.

Barnett HL, Hunder BB. 1972. Ill  streated Genera of imperfect  fungi. 22 p.

Bertagnolli BL, Dal Soglio FK, Sinclair JB. 1996. Extracellular enzyme profiles of the fungal pathogen Rhizoctonia solani isolate 2B-12 and of two antagonists, Bacillus megaterium strain B153-2-2 and Trichoderma harzianum isolate Th008.I. Possible correlations with inhibition of growth and biocontrol. Physiol Mol Plant Pathol 48, 145-160. http://dx.doi.org/org/10.1006/pmpp. 1996. 0013.

Bhattacharya D, Dhar TK,  Siddiqui KA, Ali E. 1992. Phytotoxic metabolites of Macrophomina phaseolina. Indian Journal of Mycology and Plant Pathology 22, 54–57.

Bhattacharya D, Dhar TK,  Siddiqui KA, Ali E. 1994. Inhibition of seed germination by Macrophomina phaseolina is related to phaseolinone production; Journal of Applied Bacteriology 77, 129–133. http://dx.doi.org/10.1111/j.13652672.1994.tb03055.x.

Binagwa PH, Bonsi CK, Msolla SN, Ritte II. 2016. Morphological and molecular identification of Pythium spp. isolated from common beans (Phaseolus vulgaris) infected with root rot disease. African Journal of Plant Science 10(1), 1-9. http://dx.doi.org/10.5897/AJPS2015.1359.

Buruchara RA. 2006. Background information on common beans (Phaseolus vulgaris L). Biotechnology, breeding and seed systems for African Crops; [Online] Available (accessed 23rd July 2012). The Rockefeller Foundation, Nairobi, Kenya. www.africancrops.net/rockefeller/crops/beans/index.htm

Choi HW, Hwang BK. 2011. Systemic  acquired resistance of pepper to microbial pathogens. Journal of  Phytopathol 159, 393-400. http://dx.doi.org/10.1111/j.1439-0434.2010.01781.

El-Mougy NS. 2004. Preliminary evaluation of salicylic acid and acetylsalicylic acid efficacy for controlling root rot disease of lupine under greenhouse conditions. Egyptian Journal of Phytopathology 32(1-2), 11-21.

El-Mougy NS, El-Gamal NG, Abdel-Kader MM. 2007. Control of wilt and root rot incidence in Phaseolus vulgaris L. by some plant volatile compounds.  Journal of Plant Protection Research 47 (3), 255-265.

Gomaa FH. 2010. Studies on root rot in the faba bean. Thesis, Plant Diseases, College of Agriculture, University of Alexandria, Egypt.

Hasan AK, Samir SH. 2007. Effect of Copper and Silicon Nutrients and Salicylic Acid to Induce Systemic Resistance for Cucumber Plants Against Pythium aphanidermatum (Edson) Fitz. Arab J. Pl. Prot 25, 171-174.

Hassan AK. 2013. Evaluate The Efficiency Of Some Biological And Chemical Agents In Controlling Damping Off and Root Rot Caused By Pythium aphanidermatum in Pepper. College of Agriculture-University of Baghdad, Plant Protection-Plant Pathology.

Hemeda AH. 2009. Control of bean root rot disease by using resistance chemical inducers. Alex. Journal Agriculture Res. 54(1), 165-174 www.dx.doi.org/10,3923/pp2009.79.89

Juber KS, Al-Juboory HH, Al-Juboory SB. 2016.Identification And Control Of Strawberry Root And Stalk Rot In Iraq, International Journal of Environmental & Agriculture Research 2(2), 54-63.

Kumar NP, Sharma V. 2013. Detached Leaf Assay for Resistance to Macrophomina phaseolina and Isolation of Toxin from Infected Leaves and its Analysis by TLC. Journal of Biological and Chemical Research 30(1), 254-263. http://dx.doi.org/11/04/2013Accepted:13/04/2013.

Leslie JF, Summerell BA, Bullock S. 2006. The Fusarium laboratory manual: Wiley Online Library. ISBN: 978-0-813-81919-8.

Lozovaya VV, Lygin AV, Zernova OV, Li S, Widholm JM, Hartman GL. 2006. Lignin degradation by Fusarium solani f.sp. glycines. plant Dis  9, 77-82. http://dx.doi.org/10.1094/PD-90-0077

Maina PK,  Wachira PM, Okoth SA, Kimenju JW. 2017. Cultural, Morphological and Pathogenic Variability among Fusarium oxysporum f. sp. phaseoli Causing Wilt in French Bean (Phaseolus vulgaris L.). Journal of Advances in Microbiology 2(4), 1-9. www.dx.doi.org/10.9734/JAMB/2017/32684

Marcenaro D, Valkonen JP.  2016. Seedborne Pathogenic Fungi in Common Bean (Phaseolus Vulgaris Cv. Inta Rojo) In Nicaragua. Plos One, 11(12), E0168662. December 20, 2016. www.dx.doi.org/10.1371/journal.pone.0168662

Matloob AAH. 2012. Determination of The Causeses of Bean Foot and Root Rot Disease and Evaluation of the Efficacy of Some Biocontrol Agents in Their Control, Plant Protection, Plant Diseases, College of Agriculture at the University Of Baghdad.

Mbazia A, Ben Youssef NO, Kharrat M. 2016. Effect of some chemical inducers on chocolate spot disease of faba bean in Tunisia. J. Crop Prot 5(4), 541-552. www.dx.doi.org/10.18869/modares.jcp.5.4.541.

Morris MM, Muthomi JW, Wagacha JM. 2017. Effect of Soil Fertility and Intercropping on the Incidence and Severity of Root Rot Diseases of Common Bean (Phaseolus vulgaris L.). World Journal of Agricultural Research 5(4), 189-199. http://dx.doi.org/10.12691/wjar-5-4-1.

Mwang ombe AW, Thiong G, Olubayo FM, Kiprop EK. 2007. Occurrence of Root Rot Disease of Common Bean (Phaseolus vulgaris L.) In Association with Bean Stem Maggot (Ophyiomia sp.) In EMBU District, Kenya. Plant Pathology Journal 6, 141-146. http://dx.doi.org/ppj.2007.141.146.

Nicolai P, Boy E,  Wirth JP, Hurrell RF. 2015. Review: The Potential of the Common Bean (Phaseolus vulgaris) as a Vehicle for Iron Biofortification Nutrients 7(10), 1144-1173. http://dx.doi.org/10.3390/nu7021144

Parmeter J, Whitney HS. 1970. Taxonomy and nomen cleature of the imperfect stage in: Rhizoctonia solani Biology and pathology. parmeter, J.R. Univ. of  California , Berkeley. 255 p.

Salari M, Panjekeh N,  Nasirpoor Z, Abkhoo J. 2012. Reaction of melon (Cucumis melo L.) cultivars to soil-borne plant pathogenic fungi in Iran. African Journal of Biotechnology 11(87), 15324-15329. http://dx.doi.org/10.5897/AJB12.799.

Saseetharan NHM, Zakaria L. 2014. Occrrence of Fusarium spp. On vegetable Crop and Assessment of their pathogenicity pertanika. J Trop. Agric. Sci 37, 445 – 455.

Takemura Y, Satoh M, Satoh K, Hamada H,  Sekido Y, Kubota S. 2010. High dose of ascorbic acid induces cell death in mesotheiioma cells. Biochem. Biophys. Res. Commun 394, 249–253. http://dx.doi.org/10.1016/j.bbrc.2010.02.012

Timothy GP, James SB, Daniel GD, Scott AJ, James DK, Hannes D. 2013. Use of Wild Relatives and Closely Related Species to Adapt Common Bean to Climate Change. Agronomy 3, 433-461. http://dx.doi.org/10.3390/agronomy3020433.

Uquillas  C, Letelier I, Blanco F, Jordana X, Holnigne L. 2004. NPR1-Idndependent activation of immediate early salicylic acid responsive genes in Arabidopsis. Molecular Plant Microbe 17(1), 34-42. http://dx.doi.org/10.1094/MPMI.2004.17.1.34

Vidhyasekaran P. 1997. Fungal Pathogenesis in plant in crop. Molecular biology and host defense mechanism. Marcel Dekker Incorportion, 2nd Edition. 542 p.