Evaluation of the healing activity of propolis hydrogel in an excision-based model

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Research Paper 06/11/2022
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Evaluation of the healing activity of propolis hydrogel in an excision-based model

Emmanuel Agbamu, Matthew Ikhuoria Arhewoh, Efe Endurance Ahama, Happiness Chiamaka Obeleke, Emmanuel Igho Odokuma
Int. J. Biosci.21( 5), 37-47, November 2022.
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Propolis hydrogel made of carbopol has been shown to provide better contact with the wounded site and reduce wound healing time. This study explored the physicochemical properties of Nigerian propolis hydrogel and its wound healing activity.  Propolis was obtained from bee hives using standard techniques and macerated in absolute ethanol at 40 oC for 2 weeks in a dark room at room temperature. The preparation was filtered, concentrated and stabilized in 20 ml 5 %v/v tween 80. The hydrogel was prepared at different concentrations of propolis and carbopol. The physicochemical properties of the formulation were evaluated and statistically analyzed. The animals were grouped into six; group 1 was treated with the control (cikatrin), group 2 was treated with the blank gel, group 3, 4, 5 and 6 were treated with formulation P1, P2, P3 and P4 respectively. The wound healing activity was evaluated on days 7, 14, and 21 after inducing dermal injury. The quantitative study of the extract indicated the presence of alkaloid (351 mg/g), flavonoid (300 mg/g), phenolic (50 mg/g), tannin (10.9 mg/g), and saponin (19.9 mg/g). The pH of the formulation was 6.73 – 6.6 (p = 0.04); spreadability was 4.72 – 3.17 (p = 0.64); depth was 18.67 – 14.83 (p < 0.001); viscosity was highest in formulations of 2 % carbopol (72,802-74,122 mPas.s). The stability studies revealed that preparations stored in the refrigerator had better profiles, (pH of 6.86-4.51; spreadability of 3.25-5.57; viscosity of 7997-72,802 mPas.s) over 90 days period. The histoarchitectural features were characterized by ulceration, granulation tissue formation, and various shades of wound healing. This study has shown that propolis formulated as hydrogel can be used to induce wound healing, and thus indicated the need for standardizing propolis content in wound dressings and other topical delivery systems to obtain optimum activity with good physicochemical profile.


Akanksha D, Vikas G, Neetesh KJ, Shalendra S, Neelam B, Dinesh KJ. 2009. Formulation and Evaluation of Neomycin Sulphate Ointment containing Natural Wound Healing Agent Curcuma longa, International Journal of Pharmaceutical Sciences and Drug Research 1, 105–118.

Alalor CA, Okafo SE, Onyeisi J. 2021. The formulation and evaluation of coconut oil-based based diclofenac-loaded solid self-emulsifying drug delivery system. African Journal of Biomedical Research 24(2), 181-186

Alaribe CS, Oladipupo AR, Ola AO, Okeoma C, Adeyeye AO, Basheeru KA, Luca R, Coker HAB. 2018. Comparative Chemical and Quantitative Analysis of Flavonoid Contents In Propolis Samples From South East (Abia) and South West (Ibadan) of Nigeria. Nigerian Journal of Pharmaceutical and Applied Science Research 7(2), 19-27.

Arhewoh MI, Agbamu E, Agare GI, Enwa FO, Aduba P, Atamenwan OJ. 2022. Evaluation of the Antimicrobial Activity of Propolis Ointment. Nigerian Journal of Pharmaceutical Research: In press 18(2).

Bankova VS, De Castro SL, Marcucci MC. 2000. Propolis: Recent advances in Chemistry and Plant Origin. Apidologie 31(1), 3–15.

Baranda L, Gonzalez-Amaro R, Torress-Alvarez B, Alvarez C, Ramirez V. 2000. Correlation between pH and irritant effect of cleansers marketed for dry skin. International Journal of Dermatology 41, 494-499.

Chin GC, Diegelmann RF, Schultz GS. 2005. Cellular and molecular regulation of wound healing. In: Falabella AF, Kirsner RS, editors. Wound healing. Boca Raton: Taylor & Francis Group; 17-37 p.

Chirani N, Yahia L, Gritsch L, Motta FL, Chirani S, Fare S. 2015.  History and application of hydrogels. Journal of Biomedical Science 4, 1-6.

Dash GK, Murthy PN. 2011. Evaluation of Argemone mexicana Linn. Leaves for Wound Healing Activity. Journal of Natural Product and Plant Resources 1(1), 46–56.

Drescher N, Klein AM, Schmitt T, Leonhardt SD. 2019. A clue on bee glue: New insight into the sources and factors driving resin intake in honeybees (Apis mellifera). PLOS one 14(2), p.e0210594.

Działo M, Mierziak J, Korzun U, Preisner M, Szopa J, Kulma A. 2016. The potential of plant ohenolics in prevention and therapy of skin disorders. International Journal of Molecular Science, 17, 160-162

Eming SA, Krieg T, Davidson JM. 2007.  Inflammation in wound repair. Molecular and cellular mechanisms. Journal of Investigative Dermatology, 127, 514-516.

Trease GC, Evans WC (1996). Trease and Evans Pharmacognosy, 14th Ed. London: W.B. Sanders. pp. 545-546.

Jin K, Chang ML. 2018. Transdermal hydrogel composed of polyacrylic acid containing propolis for wound healing in a rat model. Macromolecular Research 26(13), 1219-1224.

Kaler A, Mittal AK, Katariya M, Harde H, Agrawal AK, Jain S, Banerjee UC. 2014. An investigation of in vivo wound healing activity of biologically synthesized silver nanoparticles. Journal of Nanoparticle Research 16, 2605-2607.

Kokane D, More RY, Kale MB, Nehete MN, Mehendale PC, Gadgoli CH. 2009. Evaluation of wound healing activity of root of Mimosa pudica. Journal of Ethnopharmacology 124(2), 311–315.

Maria LAB, Ricardo LSH, Lucia MC, Vânia SA, Eliana MMR, Rosangela PLL. 2011. Antimicrobial and Wound Healing Activities of Piper hayneanum. Journal of Chemical and Pharmaceutical Research, 3(4), 213–222.

McLennan SV, Bonner J, Milne S, Lo L, Charlton A, Kurup S, Jia J, Yue DK, Twigg SM. 2008. The anti-inflammatory agent propolis improves wound healing in a rodent model of experimental diabetes. Wound Repair and Regeneration 16(5), 706-713. http://dx.doi.org/10.1111/j.1524-475X.2008.00421.x.

Moreira L, Dias LG, Pereira JA, Estevinho L. 2008. Antioxidant properties, total phenols and pollen analysis of propolis samples from Portugal. Food Chemistry and Toxicology 46(11), 3482-3485.

National Research Council. 2010. Guide for the care and use of laboratory animals, National Academies Press. Washington, DC; 2010.

Olczyk P, Komosinska-Vassev K, Winsz-Szczotka K, Stojko J, Klimek K, Kozma EM. 2013. Propolis induces chondroitin/dermatan sulphate and hyaluronic acid accumulation in the skin of burned wound. Evidence-Based Complimentary and Alternative Medicine 290675.

Omar RMK, Igoli J, Gray AI, Ebiloma GU, Clements CJ, Fearnley J, Ebel RE, Zhang T, De Koning HP, Watson DG. 2016. Chemical characterization of Nigerian red propolis and its biological activity against Trypanosoma Brucei. Phytochemical Analysis 27(2), 107–115.

Omar R, Igoli JO, Zhang T, Gray AI, Ebiloma GU, Clements CJ, Fearnley J, Ebel RE, Paget T, De Koning HP, Watson DG. 2017. The chemical characterization of Nigerian propolis samples and their activity against Trypanosoma Brucei. Science Reports 7(1), 923.

Oroian M, Florian D, Florin U. 2019. Comparative evaluation of maceration, microwave and ultrasonic-assisted extraction of phenolic compounds fro propolis. Journal of Food Science and Technology-Mysore 57(1), http://dx.doi.org/10.1007/s13197-019-04031-x

Piccinelli AL, Campo FM, Cuesta-Rubio O, Márquez HI, de Simone F, Rastrelli L. 2005. Isoflavonoids isolated from Cuban propolis. Journal of Agricultural and Food Chemistry 53, 9010-9016.

Pillai SI, Kandaswamy M, Subramanian S. 2010. Antiulcerogenic and ulcer healing effects of Indian propolis in experimental rat ulcer models. Journal of ApiProduct and ApiMedical Science 2(1), 21-28.

Sasutjarit RA, Sirivat A, Vayumhasuwan P. 2005. Viscoelastic properties of carbopol 940 gels and their relationship to piroxicam diffusion coeeficients in gel bases. Pharmaceutical Research 22, 2134-2140. http://dx.doi.org/10.1007/s11095-005-8244.

Sehn E, Hernandes L, Franco SL, Goncalves CC, Baesso ML. 2009. Dynamics of reepithelialisation and penetration rate of a bee propolis formulation during cutaneous wounds healing. Anal Chim Acta 635(1), 115–120.

Sforcin JM. 2016. Biological properties and therapeutic applications of propolis. Phytotherapy Research 30, 894–905.

Silva GL, Lee I, Douglas KA. 1998. Special problems with extraction of plants. In Cannell J. P. R (eds). Natural Products Isolation. Humana Press Publishers, New Jersey (USA) 356-358 p.

Szliszka E, Kucharska AZ, Sokol-Letowska A, Mertas A, Czuba ZP, Krol W. 2013. Chemical composition and anti-inflammatory effect of ethanolic extract of Brazilian green Propolis on activated J774A.1 Macrophages. Evidence Based Complimentary Alternative Medicine 976415.

Toreti VC, Helia HS, Maria GP, Yong KP. 2013. Recent Progress of Propolis for it’s Biological and chemical compositions and it’s Botonical Origin. Propolis: Properties, Application and it’s Potential. Evidenced Based Complementary and Alternative Medicine 697390. http://dx.doi.org/10.1155/2013/697390.

Vijay KS, Praveen KS, Purnendu KS, Peeyush KS, Ashutosh M. 2013. Formulation and evaluation of topical gel of aceclofenac containing piparine. Indo American Journal of Pharmaceutical Research 3(7), 1-15.

Zhang J, Shen X, Wang K, Cao X, Zhang C, Zheng H, Hu F. 2016. Antioxidant activities and molecular mechanisms of the ethanol extracts of Baccharis propolis and Eucalyptus propolis in RAW64. 7 cells. Pharmaceutical Biology 54(10), 2220-2235.