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Evaluation of thrombolytic, antimicrobial and cytotoxicity potential of extract and its polar fractions of Heliotropium strigosumby in-vitro assay

Abdur Rehman, Sami Ullah, Raja Adil Sarfraz, Faisal Nawaz, Abdul Qayyum, Muhammad Javid, Syed Muhammad Imran Bin Riaz, Khalil Ullah Khan

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Int. J. Biosci.10(1), 109-116, January 2017

DOI: http://dx.doi.org/10.12692/ijb/10.1.109-116


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Medicinal plants are the major sources of natural product and are known as natural source of derived compounds which are being used in the safety of human life. In the early age of human life, the plants are used for the nutritional purposes but as the research is focus on the investigation of their medicinal potential then these are become a useful source of remedies for various diseases and health improvements. The present work was focus on evaluation of antimicrobial, cytotoxicity and thrombolytic potential of methanolic extract and its polarities based fractions of Heliotropium strigosum. The extraction was performed by methanolic solvent and fractionation by using chloroform, ethyl acetate and aqueous solvent sequentially on the basis of their polarities. Antibacterial activity and antifungal activity was performed by disk inhibition assay which showed significant (p<0.05) potential 36mm and 32mm was shown by against Staphylococcus aurous and Escherichia coli respectively.  Highest antifungal activity was shown by 28mm by aqueous fractions whereas least 12mm by ethyl acetate fraction against the Aspergilus nigerfungus species.  Cytotoxicity activity also showed significant results by hemolytic assay. Thrombolytic activity showed highest activity (34.54%) by methanolic crude extract while ethyl acetate fraction showed lowest (23.16%) activity. This study showed that the crude extract and polar fractions of crude methanolic extract of H. strigosum can be considered the potential sources of antimicrobial and thrombolytic agent not only in the food industry but also in cosmetics and pharmaceutical preparations.


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Evaluation of thrombolytic, antimicrobial and cytotoxicity potential of extract and its polar fractions of Heliotropium strigosumby in-vitro assay

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