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Evaluation of tolerance potential of Aspergillus niger and Trichoderma viridae against hexvalent chromium

Gul Zareen Ghafoor, Maham Sarfraz, Maria Abbas, Memuna Ghafoor Shahid

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Int. J. Biosci.14(4), 35-44, April 2019

DOI: http://dx.doi.org/10.12692/ijb/14.4.35-44


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In this study bioremediation potential of fungi inhabiting in heavy metal contaminated soil of Aik drain was investigated. Out of the total nine species isolated, belonging to various genera, Aspergillus niger and Trichoderma viridae were selected to evaluate their potential to tolerate Cr(VI). For this purpose, PDA medium was supplemented with 100 – 1000 ppm concentration of Cr(VI) and change in the radial growth of mycelium and tolerance index (TI) of A. niger and T. viridae was investigated and compared with control (labeled as 0ppm). A decreasing trend was observed in the radial growth and TI of both isolates with the increase in metal toxicity. No visible growth in the mycelia was observed at 1000 ppm (Minimum Inhibitory Concentration). A negatively significant relationship of chromium stress was observed with radial growth of mycelia and TI at p <0.01. Among both isolates, a minor difference was observed in their TI but comparatively Aspergillus niger exhibited more ability to tolerate high concentration of chromium than Trichoderma viridae. It is concluded that the Aspergillus niger and Trichoderma viridae both can be considered as best candidates for bioremediation due to their capability to remove heavy metals from contaminated soils.


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Evaluation of tolerance potential of Aspergillus niger and Trichoderma viridae against hexvalent chromium

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