Welcome to International Network for Natural Sciences | INNSpub

Evaluation of tolerance potential of Aspergillus niger and Trichoderma viridae against hexvalent chromium

Research Paper | April 1, 2019

| Download 7

Gul Zareen Ghafoor, Maham Sarfraz, Maria Abbas, Memuna Ghafoor Shahid

Key Words:

Int. J. Biosci.14( 4), 35-44, April 2019

DOI: http://dx.doi.org/10.12692/ijb/14.4.35-44


IJB 2019 [Generate Certificate]


In this study bioremediation potential of fungi inhabiting in heavy metal contaminated soil of Aik drain was investigated. Out of the total nine species isolated, belonging to various genera, Aspergillus niger and Trichoderma viridae were selected to evaluate their potential to tolerate Cr(VI). For this purpose, PDA medium was supplemented with 100 – 1000 ppm concentration of Cr(VI) and change in the radial growth of mycelium and tolerance index (TI) of A. niger and T. viridae was investigated and compared with control (labeled as 0ppm). A decreasing trend was observed in the radial growth and TI of both isolates with the increase in metal toxicity. No visible growth in the mycelia was observed at 1000 ppm (Minimum Inhibitory Concentration). A negatively significant relationship of chromium stress was observed with radial growth of mycelia and TI at p <0.01. Among both isolates, a minor difference was observed in their TI but comparatively Aspergillus niger exhibited more ability to tolerate high concentration of chromium than Trichoderma viridae. It is concluded that the Aspergillus niger and Trichoderma viridae both can be considered as best candidates for bioremediation due to their capability to remove heavy metals from contaminated soils.


Copyright © 2019
By Authors and International Network for
Natural Sciences (INNSPUB)
This article is published under the terms of the Creative
Commons Attribution Liscense 4.0

Evaluation of tolerance potential of Aspergillus niger and Trichoderma viridae against hexvalent chromium

Abbas M, Rahman MAU, Safdar A. 2012. Detection of Heavy Metals Concentration Due to Leather Tanning Industry and Prevalent Disease Pattern in Kasur, Pakistan. Environment and Urbanization ASIA 3(2), 375-384.

Abbas ST, Mehdi SM, Sarfraz M, Hassan G. 2004. Contents of heavy-metals in waters of nullahs dek, bisharat and aik, and their effect on soil-health. Science Vision 9, 1-11.

Akhtar S, Mahmood-ul-Hassan M, Ahmad R, Suthor V, Yasin M. 2013. Metal tolerance potential of filamentous fungi isolated from soils irrigated with untreated municipal effluent. Soil and Environment 32(1), 55-62.

Akpor OB, Musa JO, Babalola OO, Adejobi OI. 2015. Tolerance of Aspergillus niger to selected concentrations of metals and sodium chloride. IOSR Journal of Environmental Science, Toxicology and Food Technology 9(10), 33-37.

Bai SR, Abraham E. 2003. Studies on chromium (VI) adsorption-desorption using immobilized fungal biomass. Bioresource Technology 87, 17 – 26.

Desai H, Patel D, Joshi B. 2016. Screening and Characterization of Heavy Metal Resistant Fungi for its Prospects in Bioremediation of Contaminated Soil. International Journal of Current Microbiology and Applied Science 5(4), 652-658.

Ezzouhri L, Castro E, Moya M, Espinola F, Lairini K. 2009. Heavy metal tolerance of filamentous fungi isolated from polluted sites in Tangier, Morocco. African journal of microbiology research 3(2), p 35-48.

Fernández PM, Viñarta SC, Bernal AR, Cruz EL, Figueroa LIC. 2018. Bioremediation strategies for chromium removal: current research, scale-up approach and future perspectives. Chemosphere 208, 139-148. https://doi.org/10.1016/j.chemosphere.2018.05.166

Fernandez PM, Farina JI, Figueroa LIC. 2010. The significance of inoculum standardization and cell density on the Cr(VI) bioremediation by environmental yeast isolates. Water, Air &Soil Pollution 212, 275-279. https://doi.org/10.1007/s11270-010-0341-0

Ghosh A, Dastidar MG, Sreekrishnan TR. 2017. Bioremediation of chromium complex dyes and treatment of sludge generated during the process. International Biodeterioration and Biodegradation 119, 448-460. http://dx.doi.org/10.1016/j.ibiod.2016.08.013

Go P. 2016. The Punjab gazette. Government of the Punjab. Law and Parliamentary Affairs.

Gupta R, Ahuja P, Khan S, Saxena RK, Mohapata H. 2000. Microbial biosorbents: meeting challenges of heavy metal pollution in aqueous solutions. Current Science 78, 967-973.

Gururajan K, Belur PD. 2018. Screening and selection of indigenous metal tolerant fungal isolates for heavy metal removal. Environmental Technology and  Innovation 9, 91-99.

Hajieghrari B. 2010. Effect of some metal-containing compounds and fertilizers on mycoparasite Trichoderma species mycelia growth response. African Journal of Biotechnology 9(26), 4025-4033.

Humber RA. 1997. Fungi: identification. In Manual of techniques in insect pathology. 153 p.

Iram S, Parveen K, Usman J, Nasir K, Akhtar N, Arouj S, Ahmad I. 2012. Heavy metal tolerance of filamentous fungal strains isolated from soil irrigated with industrial wastewater. Biologija 58(3).

Iram S, Zaman A, Iqbal Z, Shabbir R. 2013. Heavy Metal Tolerance of Fungus Isolated from Soil Contaminated with Sewage and Industrial Wastewater. Polish Journal of Environmental Studies 22(3).

Jobby R, Jha P, Yadav AK, Desai N. 2018. Biosorption and biotransformation of hexavalent chromium [Cr (VI)]: a comprehensive review. Chemosphere 207, 255-266. https://doi.org/10.1016/j.chemosphere.2018.05.050

Kannangara S, Dharmarathna RMGCS, Jayarathna DL. 2017. Isolation, identification and characterization of Trichoderma species as a potential biocontrol agent against Ceratocystis paradoxa. Journal of Agriculture Science 12(1).

Liu L, Li W, Song W, Guo M. 2018. Remediation techniques for heavy metal-contaminated soils: Principles and applicability. Science of the Total Environment 633, 206-219.

Liu SH, Zeng GM, Niu QY, Liu Y, Zhou L, Jiang LH, Tan XF, Xu P, Zhang C, Cheng M. 2017. Bioremediation mechanisms of combined pollution of PAHs and heavy metals by bacteria and fungi: A mini review. Bioresource Technology 2I24, 25-33.

Mohammadian E, Ahari AB, Arzanlou M, Oustan S, Khazaei SH. 2017. Tolerance to heavy metals in filamentous fungi isolated from contaminated mining soils in the Zanjan Province, Iran. Chemosphere185, 290-296. http://dx.doi.org/10.1016/j.chemosphere.2017.07.022

Morales-Barrera L, Cristiani-Urbina E. 2008. Hexavalent chromium removal by a Trichoderma inhamatum fungal strain isolated from tannery effluent. Water, Air &Soil Pollution 187, 327-336.

Oladipo OG, Awotoye OO, Olayinka A, Bezuidenhout CC, Maboeta MS. 2018. Heavy metal tolerance traits of filamentous fungi isolated from gold and gemstone mining sites. Brazilian Journal of Microbiology 49(1), 29-37. https://doi.org/10.1016/j.bjm.2017.06.003

Qadir A, Malik RN, Husain SZ. 2008. Spatio-temporal variations in water quality of Nullah Aik-tributary of the river Chenab, Pakistan. Environmental Monitoring and Assessment 140(1), 43-59.

Rasool A, Irum S. 2014. Toxic metal effect on filamentous fungi isolated from the contaminated soil of Multan and Gujranwala. Journal of Bioresource Management 1(2), 1.

Sugasini A, Rajagopal K. 2015. Hexavalent chromium removal from aqueous solution using trichoderma viride. International Journal of Pharma and Biosciences 6(1), 485-495.