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Evaluation of wild Rhynchosia minima (L.) DC. through morphometric and biochemical markers

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Research Paper 01/03/2019
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Evaluation of wild Rhynchosia minima (L.) DC. through morphometric and biochemical markers

Arshad Khan, Murad Ali, Rashid Ul-Haq, Khalid Khan, Karishma Gul, Aizaz Khan, Imran Khan, Sundas Wisal, Mazhar Iqbal
Int. J. Biosci.14( 3), 7-15, March 2019.
Certificate: IJB 2019 [Generate Certificate]
Key: BiochemicalRhynchosia minima morphometricSDS-PAGE

Abstract

Aim of the study, to explore the intra-specific diversity among the genotypes of specie Rhynchosia minima. The methods to study genetic variation are morphological and SDS-PAGE. Total 13 morphological characters was studied which include both qualitative and quantitative traits. Significant level of variation was observed in four qualitative traits, two types of pod shape was found (70.8%) curved and (20.1%) oval. In case of seed color three colors were observed (54.1%) grey, (37.5%) dark grey and (8.3%) light grey. Leaf color and seed shape showed no variation. Descriptive statistics done for nine quantitative traits in which seed per pod showed maximum coefficient of variance CV (173.66%) followed by petiole length (49.62%), biomass per plant (46.21%) and pod per plant (45.65%). While minimum coefficient of variance showed by leaf width (25.62%) followed by internode length (29.48%), leaf length (30.80%) and pod length (34/68%). Comparative correlation was also done in which petiole length show strongly negative correlation with internode length and biomass per plant. One way cluster analysis for nine quantitative traits also done which separated the genotypes into different cluster on the basis of genetic similarity and dissimilarity. SDS-PAGE was carried out on 12% gel electrophoresis which revealed 10 reproducible bands. High level of variation was observed in band B-1 (0.54%) followed by B-2 (0.54%), B-3 (0.46%) and B-5 (0.42%), similarly low level of variation was found in B-7 (0.25%) followed by B-4 (0.29%) and B-8 (0.33%).The entire bands loci show polymorphism. report addressing genetic variability in R. minima.

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