Evaluation the efficiency of chitosan and salicylic acid in controlling gray mold disease caused by Botrytis cinerea on eggplant

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Research Paper 01/02/2019
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Evaluation the efficiency of chitosan and salicylic acid in controlling gray mold disease caused by Botrytis cinerea on eggplant

Hind Basem Hattab Al-Hadithy, Hurria Hussien Al-Juboory
J. Bio. Env. Sci.14( 2), 9-16, February 2019.
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Abstract

The study was conducted to isolate and identify the causal agent of gray mold disease on eggplant and evaluate the efficiency of chitosan (CH) and salicylic acid (SA) to control the disease. The morphological characteristics proved that the causal agent of gray mold on eggplant is Botrytis cinerea . Three isolates of the pathogen, from sample showing gray mold at different location of Baghdad area, were obtained and designated as BC1, BC2, BC3. The pathogenicity tests on wounded eggplant fruits revealed that the 3 isolates were highly pathogenic. The disease severity was found to be 90.25 , 83.21 , 69.52% followed by BC2 with disease severity , 72.20 , 66.39 , 57.20 % and BC1 , 56.20 , 56.72 , 39.59 % after 14 days of inoculation on wounded fruits inoculates on upper third , lower third , and on unwounded fruits respectively. The addition of chitosan at 300 mg / L and SA at 125 mg / L separately or in combination into PDA caused high reduction in B. cinerea growth. The combination of SA + CH was the more effective with growth inhibition percentage 95.29% compared with 87.84% and 87.83% with SA and CH respectively. The application of SA and Chitosan on the plants 7 days before leaves inoculation with BC3 caused signification reduction in disease severity to 4.23% compared with 55.6% in control.

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Al-Maamoory MWH. 2013. Pathogencity and Vitality isolated from the fungus Botrytis cinerea in some of the genotypes of the tomato. Athesis, College of Agriculture. University of Baghdad.

Al-Rekabi FE, Abd El-Jabar Jasem. 1981. Vegetable production, Institute of Technical Institutes. Ministry of Higher Education and Scientific Research, Baghdad. Iraq.

Aziz A, Trotel-Aziz P, Dhuicq L, Jeandet P, Couderchet M, Vernet G. 2006. Chitosan oligomers and copper sulfate induce grapevine defense reactions and resistance to gray mold and downy mildew. Phytopathology 96, 1188–94.

Bentez T, Rincon AM, Limon MC, Codon AC.  2004. Biocontrol   mechanisms of Trichoderma strains. Internationla Microbiol 7, 249 -260.

Choquer M, Fournier E, Kunz C, Levis C, Pradier JM, Simon A, Viaud M. 2007. Botrytis cinerea virulence factors: New insights into a necrotrophic and polyphagous pathogen. FEMS. Federation of European, Microbiological Societies.  Microbiology. Letters 277, 1-10.

Dean R, Van KJAPretorius ZAHammond-Kosack KEDi Pietro ASpanu PDRudd JJDickman MKahmann REllis JFoster GD. 2012. The top 10 fungal pathogens in molecular plant pathology. Molecular Plant Pathology 13, 414–430.

Di Piero N, Garda A. 2008. Quitosana reduz a severidade da antracnose e aumenta a atividade de glucanase em feijoeiro-comum. Pesq Agropec Bras, 43, 1121-1128.

EIGhaouth A, Arul J, Asselin A. 1992. Antifungal activity of chitosan on two postharvest pathogens of strawberry fruits. Phytopathology 82, 398-402.

Ellis MB. 1971. Dematiaceous Hyphomycetes. 1st Edn., Commonwealth Mycologicl Institute, Kew, Surrey, UK., 608.

Elmer PAG, Reglinski T, Taylor JT, Wood PN,  Hoyte SM. 2010. Inhibition of Botrytis cinerea growth and suppression of botrytis bunch rot in grapes using Chitosan plant pathology 59, 882-890.

Farhan R, Al-Juboory HHH. 2018. Evaluation of the efficiency of some antioxidant chemical for germination of bean seeds and on the inhibiting growth of the pathogen causing root and stalk rot disease under laboratory. Journal of Biodiversity and Environmental Sciences 12(3), p 98-109.

Fillinger S, Elad Y. 2016. Botrytis-the Fungus, the Pathogen and its Management in Agricultural Systems. Pub. Springer, New York.

Freeman S, Minz D, Kolesnik I, Barbul O, Zveibil A, Maymon M. 2004. Trichoderma biocontrol of Colletotric humacutatum and Botrytis cinerea and survival in strawberry. European Journal of Plant Pathology 110, 361-370.

Grabke A, Fernandez-Ortuno D, Schnabel G. 2013. Fenhex amid resistance in Botrytis cinerea from strawberry fields in the carolinasis associated with four target gene mutation. Plant Disease 97, 271-276.

Grabke A, Fernandez_Ortuno D, Amiri A, Li XP, Peres NA, Smith P, Schnabel G. 2014. Characterization of iprodion resistance in Botrytis cinerea from strawberry and blackberry. Phytopathology 104, 396-402.

Hayfa Jabnoun-Khiareddine, Riad SR, El-Mohamedy, Farid Abdel-Kareem, Rania Aydisen Abdalla, Mouna Gueddes-chahed, Majda Daami-Remadi. 2015. Variation in Chitosan and Salicylic Acid Efficacy Towards Soil-borne and Air-borne Fungi and their suppressive Effect of Tomato Wilt Severity. Plant pathol microbial 6, 11.

Hussan AK. 2005. Evaluation of Efficiency for some induced factors and fungicides in protection of cucumber plants against (Pythium aphanidermatum). Athesis, College of Agriculture. University of Baghdad.

Karabulut OA, Gabler FM, Mansour MM, Smilanick JL. 2004. Postharvest ethanol and hot water treatments of table grapes to control gray mold. Postharvest Biology and Technology 34, 169–177.

Kubicek CP, Starr TL, Glass NL. 2014. Plant cell wall-degrading enzymes and their secretion in plant-pathogenic fungi. Annual Review of Phytopathology 52, 427–451.

Li L, Zou Y. 2017.  Induction of disease resistance by salicylic acid and calcium ion against Botrytis cinerea in tomato (Lycopersicon esculentum), Emirates Journal of Food and Agriculture 29(1), 78-82.

Martín AF, Fernández JG, Pereira SV, Messina GA, Salinas E, Raba J, Ferramola MIS. 2011. Development of an indirect competitive enzyme linked immunosorbent assay applied to the Botrytis cinerea quantification in tissues of postharvest fruits. Fernández-Baldo et al. BMC Microbiology.

Mckinney HH. 1923. Influence of soil temperature and moisture on infection of wheat seedling by Helminthosporum sativum. Journal of Agriculture. Research 26, 195-217.

Metrau JP. 2001. Systemic acquired and Salicylic acid. curvet and knowledge European Journal of Plant Pathology 106, 13 -18.

Mosbach A, Leroch M, Mendgen KW, Hahn M. 2011. Lack of evidence for a role of hydrophobins in conferring surface hydrophobicity to conidia and hyphae of Botrytis cinerea. Microbiology 11, 10-21.

Nicot PC. 2008. Protection intégrée des cultures maraîchères sous serre: expérience et atouts pour un contexte en évolution. Cahiers Agricultures 17(1), 45-50.

Ramachandraw M, Rao VR. 1980. Physiological analysis of nitrogen response in safflower. Indian Journal Agriculture science 50(12), 918–924.

Reglinski T, Elmera PAG, Taylora JT, Woodb PN, Hoyte SM. 2010. Inhibition of Botrytis cinerea growth and suppression of botrytis bunch rot in grapes using chitosan. Plant Pathology 59, 882–890.

Romanazzi G, Nigro E, Ippolito A, Di Venere D, Salerno M. 2002. Effects of pre- and postharvest chitosan treatments to control storage grey mold of table grapes. Journal of Food Science 67, 1862–7.

Rosslenbroich HJ, Stuebler D. 2000. Botrytis cinerea history of chemical control and novel fungicides for its management. Crop Protection 19, 557–61.

Saleh YA, Al-Mansoury BAA. 2015.  Evaluation of the efficiency of bioagent Trichoderma harzianum and some fungicides for field control of gray mold disease on eggplant caused by Botrytis cinerea field. Kufa Journal of Agricultural Sciences 7(4).

Silva JS, Stamford NP, Lima MAB, Arnaud TMS, Pintado MM, Sarmento BF. 2014. Characterization and inhibitory activity of chitosan on hyphae growth and morphology of Botrytis cinerea plant pathogen. International Journal of Applied Research in Natural Products 7(4), 31-38.

Soliman HM, El-Metwally MA, Leaky MT,  Badawi WE. 2015. Alternatives to Chemical Control of Grey Mold Disease on Cucumber Caused by Botrytis cinerea Pers. Asian Journal of Plant Pathology 9, 1-15.

Trotel-Aziz P, Couderchet M, Vernet G, Aziz A, 2006. Chitosan stimulates defense reactions in grapevine leaves and inhibits development of Botrytis cinerea. European Journal of Plant Pathology 114, 405–13.

Vlot AC, Klessig DF, Park SW. 2008. Systemic acquired resistance: the elusive signal(s). Current Opinion in Plant Biology 11, 436–442.

Yin G, Wang W, Sha S, Liu L, Yu X. 2010. Inhibition and control effects of the ethyl acetate extract of Trichoderma harzianum fermented broth against Botrytis cinerea African Journal of Microbiology Research 4(15), 1647-1653,

Zhang CQ, Hu JL, Wei FL, Zhu GN. 2009. Evolution of resistance to different classes of fungicides in Botrytis cinerea from greenhouse vegetables in eastern China. Phytoparasitica 37(4), 351-359.

Zhang CQ, Hu JL, Wei FL, Zhu GN. 2009. Evolution of resistance to different classes of fungicides in Botrytis cinereafrom greenhouse vegetables in eastern China. Phytoparasitica 37(4), 351- 359.

Zimmerli L, Jakab C, Me´ traux JP, Mauch-Mani B. 2000. Potentiation of pathogen-specific defense mechanisms in Arabidopsis by beta-aminobutyric acid. Proceeding of the National Academy of Sciences of the United States of America 97, 12920–12925.