Exploration of gastric ulcer in the people of rural area of Peshawar/KPK through enzyme linked immunosorbent assay

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Research Paper 01/10/2017
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Exploration of gastric ulcer in the people of rural area of Peshawar/KPK through enzyme linked immunosorbent assay

Bashir Ahmad, Komal Aman, Sajid Ali, Shumaila Bashir, Nourin Mahmood, Muhammad idrees
Int. J. Biosci.11( 4), 365-371, October 2017.
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Abstract

Helicobacter pylori reside and grow in digestive tract of human and cause gastric ulcer which in extreme cases leads to gastric adinocarcinoma. People in most of the territories in Peshawar/Pakistan believe that H. pylori is due to different life style habits and different acidic food usage. Therefore to clear this contradiction and expose actual cause of this disease and to bring awareness among people, this study has been designed. H. pylori antibodies were determined using ELISA (Enzyme Linked Immunosorbent Assay), because this is an easy diagnostic approach and is low cost diagnostic test. This study was started from January 2016 to May 2016 and Blood sample was collected from general population of a rural area of Peshawar KPK. In this aspect we collected blood samples from 80 subjects who had stomach problem and also from 6 normal individuals as control. Gender and age base study was done in this research. Out of 80 patients, 60 patients showed positive results for H. pylori, 10 were negative and 10 showed equivocal (nor clearly positive neither negative). Moreover, female have shown more positivity as compared to male and 40-50 age group subjects were more affected. H. pylori infection is present in more than 50% of the population and bacteria are involved in causing it not people’s life style. Secondly huge population involvement reflects unawareness of the problem concerned.

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Ali N, Ullah A, Akhtar S, Shah SW, Junaid M. 2013. Factors associated with peptic ulcer: a single centre experience at tertiary care hospital of Khyber Pakhtunkhwa. KMUJ-Khyber Medical University Journal 5, DOI: 10.6007/ijarbss/v 4-i2/668.

Alley NJ, Newell DG, Ormand JE, Carpenter HA, Wilson WR, Zinsmeister AR, Perez-Perez GI, Blaser MJ. 1991. Serodiagnosis of Helicobacter pylori: comparison of enzyme-linked immunosorbent assays. Journal of Clinical Microbiology 29, 1635-1639. DOI: 10.1093/jnci/83.23.1734.

Ashraf HM, Ashraf S, Saeed F, Mehtab M, Asad S. 2005. Is it worthwhile to treat Helicobacter pylori in all dyspeptic spatients. Pak Armed Forces Med J 55, 135-140. DOI: org/10.1007/s11739-011-0614-7.

Bayerdörffer E, Oertel H, Lehn N, Kasper G, Mannes GA, Sauerbruch T, Stolte M. 1998. Topographic association between active gastritis and Campylobacter pylori colonisation. Journal of clinical pathology 42, 834-839. DOI: 10.1136/jcp.42.8.834.

Bazzoli F, Zagari M, Fossi S, Pozzato P, Ricciardiello L, Mwangemi C, Roda A, Roda E. Urea breath tests for the detection of Helicobacter pylori infection. Helicobacter 2, 34-37. DOI: 10.1111/ j.1523-5378.1997.06b10.x

De Martel C, Ferlay J, Franceschi S, Vignat J, Bray F, Forman D, Plummer M. 2012. Global burden of cancers attributable to infections in 2008: a review and synthetic analysis. The lancet oncology 13, 607-615. DOI: 10.1016/S1470-2045(12)70137-7.

Dhar R, Mustafa AS, Dhar PM, Khan MS, Al-Rashidi FJ, Al-Shamali AA, Ali FH. 1998. Evaluation and comparison of two immunodiagnostic assays for Helicobacter pylori antibodies with culture results. Diagnostic microbiology and infectious disease 30, 1-6. DOI: 10.1016/S0732-8893(97)00178-8.

Dowsett SA, Kowolik MJ. 2003. Oral Helicobacter pylori: can we stomach it. Critical Reviews in Oral Biology & Medicine 14, 226-233. Critical Reviews in Oral Biology & Medicine 2003;14:226-233 DOI: 10.1177/154411130301400307.

Evans DJ, Evans DG, Graham DY, Klein PD. 1989. A sensitive and specific serologic test for detection of Campylobacter pylori infection. Gastroenterology 96, 1004-1008. DOI: 10.1016/0016-5085(89)91616-8.

Forman D, Coleman M, De Backer G, Elder J, Møller H, Cayolla Da Motta L, Roy P, Abid L, Tjönneland A, Boeing H, Haubrich T. 1993. Epidemiology of and risk factors for, Helicobacter pylori infection among 3194 asymptomatic subjects in 17 populations. Gut 34, 1 672-1676. DOI: 10.1136/gut. 34.12.1672.

Goodwin CS, Blincow E, Peterson G, Sanderson C, Cheng W, Marshall B, Warren JR, McCulloch R. 1987. Enzyme-linked immunosorbent assay for Campylobacter pyloridis: correlation with presence of C. pyloridis in the gastric mucosa. Journal of Infectious Diseases 155, 488-494. DOI: 10.1093/infdis/155.3.488.

Graham DY, Malaty HM, Evans DG, Evans DJ, Klein PD, Adam E. 1991. Epidemiology of Helicobacter pylori in an asymptomatic population in the United States. Gastroenterol 100, 1495-1501. DOI: 10.3109/00365529108996244.

Hartgrink HH, Jansen EP, van Grieken NC. 2009.  Gastric cancer. Lancet 374, 477-490. DOI: 10.1016/S0140-6736(09)60617-6.

Hirschl AM, Pletschette M, Hirschl MH, Berger J, Stanek G, Rotter ML. 1998. Comparison of different antigen preparations in an evaluation of the immune response to Campylobacter pylori. European Journal of Clinical Microbiology & Infectious Diseases 7, 570-575. DOI: 10.1007/bf01962618.

Hunt RH, Xiao SD, Megraud F, Leon-Barua R, Bazzoli F, Van der Merwe S, Vaz Coelho LG, Fock M, Fedail S, Cohen H, Malfertheiner P. 2011. Helicobacter pylori in developing countries. World gastroenterology organisation global guideline. J Gastrointestin Liver Dis 20, 299-304. DOI: 10.1097/ mcg.0b013e31820fb8f6.

Jones DM, Eldridge J, Fox AJ, Sethi P, Whorwell PJ. 1986. Antibody to the gastric campylobacter-like organism (Campylobacter pyloridis) clinical correlations and distribution in the normal population. Journal of medical microbiology 22, 57-62. DOI: 10.1099/00222615-22-1-57.

Kanbay M, Gür G, Arslan H, Yilmaz U, Boyacioĝlu S. 2005. Does eradication of elicobacter ylori infection help normalize serum lipid and CRP levels. Digestive diseases and sciences 50, 1228-1231. DOI: 10.1007/s10620-005-2764-9.

Kleanthous H, Lee CK, Monath TP. 1998. Vaccine development against infection with Helicobacter pylori. British medical bulletin 54, 229-241. DOI: 10.1093/oxfordjournals.bmb.a011673.

Marshall B, Warren JR. 1984. Unidentified curved bacilli in the stomach of patients with gastritis and peptic ulceration. The Lancet 323, 1311-1315. DOI: 10.1016/S0140-6736(84)91816-6.

Mégraud F. 1997. How should Helicobacter pylori infection be diagnosed. Gastroenterology 113, S93-S98. DOI: 10.1016/S0016-5085(97)80020-0.

Mendall MA, Goggin PM, Molineaux N, Levy J, Northfield TC, Strachan D, Toosy T. 1992. Childhood living conditions and Helicobacter pylori seropositivity in adult life. The Lancet 339, 896-897. DOI: 10.1016/0140-6736(92)90131-R.

Newell DG, BJ Johnston, MH Ali, Reed PI. 1998. An enzyme-linked immunosorbant assay for these rodiagnosis of Campylobacter pylori-associated gastritis. Scand J Gastroenterol 23, 53-57. DOI: 10. 3109/00365528809091714.

Newton EB, Versants MR, Sepe TE. 2008. Giant duodenal ulcer. World J Gastroenterol 14, 4995-499.

Oksanen A, Veijola L, Sipponen P, Schauman KO, Rautelin H. 1998. Evaluation of Pyloriset Screen, a rapid whole-blood diagnostic test for Helicobacter pylori infection. Journal of clinical microbiology 36, 955-957. DOI: 10.1128/CVI.00165-08.

Perez-perez GI, Dworkin BM, chodos JE. 1988. Campylobacter pylori antibodies in human. Annals of internal medicin 109, 11-17. DOI: 10.7326/0003-4819-109-1-11.

Qureshi AF, Memon AS, Memon MA, Memon JM, Soomro AA, Shaikh MK. 1996. Incidence of Helicobacter pylori in gastrodoudenitis. Biomedical 12, 19-21. DOI: 10.1163/156939103322580544.

Reilly TG, Poxon V, Sanders DS, Elliott TS, Walt RP. 1997. Comparison of serum, salivary, and rapid whole blood diagnostic tests for Helicobacter pylori and their validation against endoscopy based tests. Gut 40, 454-458. DOI: 10.1136/gut.40.4.454.

Schistosomes IARC. 1994. liver flukes and Helicobacter pylori IARC Monogr Eval Carcinog Risks Hum DOI: 10.1002/ijc.2910600502.

Sitas F, Forman D, Yarnell JW, Burr ML, Elwood PC, Pedley S. 1991.  Helicobacter pylori infection rates in relation to age and social class in a population of Welsh men. Gut 32, 25-28. DOI: 10.1136/gut.32.1.25.

Smoak BL, Kelley PW, Taylor DN. 1994. Seroprevalence of Helicobacter pylori infections in a cohort of US Army recruits. American journal of epidemiology 139, 513-519 DOI: 10.1001/jama.1991. 03470190072032.

Torres J, Leal-Herrera Y, Perez-Perez G, Gomez A, Camorlinga-Ponce M, Cedillo-Rivera R, Tapia- Conyer R, Muñoz O. 1998. A community-based seroepidemiologic study of Helicobacter pylori infection in Mexico. Journal of Infectious Diseases 178, 1089-1094. DOI: 10.1086/515663.

Valliani A, Khan F, Chagani B. 2013. Factors associated with Helicobacter pylori infection, results from a developing country-Pakistan. Asian Pacific Journal of Cancer Prevention 14. 53-56.

Warren JR, Marshall B. 1983. Unidentified curved bacilli on gastric epithelium in active chronic gastritis. The lancet 321, 1273-1275. DOI: 10.1016/ S0140-6736(83)92719-8.