Exploring bacterial flora in rhizosphere of Nicotiana tabbacum at district Swabi; High tobacco production zone, Pakistan

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Research Paper 01/11/2017
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Exploring bacterial flora in rhizosphere of Nicotiana tabbacum at district Swabi; High tobacco production zone, Pakistan

Muhammad Idrees, Kalsoom, Shahzeb Wahid, Sana Mateen, Amar, Faheem Ahmad Khan, Johar Jamil
Int. J. Biosci.11( 5), 186-191, November 2017.
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Nicotiana tabbacum is an annually grown herbaceous plant promising great economic importance and the rhizosphere of Nicotiana tabbacum harbours considerable groups of microorganisms. The aim of research was to isolate and study the growth characteristics of indigenous bacterial population from the rhizosphere of Nicotiana tabbacum from different areas of district Swabi i.e. Marghuz, Kotha and Zaida and to maintain pure cultures for future reference. The soil samples were collected from rhizosphere with different age groups of the same species and brought into the lab for further processing. All samples were grown on different Media including Nutrient agar, MacConkey agar and Mannitol Salt Agar and a series of biochemical tests were performed for identification and characterization of the most commonly occurring rhizobacteria of this region. The result of colony morphology and biochemical tests confirmed the presence of four bacterial isolates i.e. Micrococcus luteus, Bacillus subtilis, Bacillus cereus and Escherichia coli. Bacillus subtilis, Bacillus cereus and Micrococcus luteus are gram positive while Escherichia coli is a gram negative bacteria. It is inferred from this study that most common isolates in rhizosphere of Nicotiana tabbacum was antibiotic producers which can be used as biofertilizer for enhancing growth and prevention of pathogens


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