Exploring the mutagenic properties of picric acid on some biochemical attributes of three Brassica napus L. cultivars

Paper Details

Research Paper 01/12/2019
Views (374) Download (16)
current_issue_feature_image
publication_file

Exploring the mutagenic properties of picric acid on some biochemical attributes of three Brassica napus L. cultivars

Ishtiaq Ahmad, Barkat Ullah
Int. J. Biosci.15( 6), 174-185, December 2019.
Certificate: IJB 2019 [Generate Certificate]

Abstract

The present study was carried out to explore mutagenic effect picric acid on some biochemical parameters including carotenoid, phenols, prolines, protein and sugar contents of three canola cultivars (Abasin-95, Dur e Nifa, Nifa Gold). Three factorial randomized complete block design was selected for the present study. Significant variation in the results of carotenoid, phenols, prolines, and protein and sugar contents in the leaves of all verities was observed using different treatment levels of picric acid. Similarly, priming durations also exhibited significant results for all the parameters except leaf phenols and proteins contents. Moreover, ANOVA for inter cultivar variations was also found significant for various biochemical parameters of the three canola cultivars excluding protein contents. These results suggest that all the three cultivars possess different genotypes. It is obvious from the data that picric acid treatment levels reduced the leaf carotenoid and phenol contents of canola plants as compared to control. On the other hand, lower doses of picric acid were stimulatory for leaf proline and sugar contents. Among different picric acid priming durations, plants raised from seeds pre-soaked with 6 hours duration proved good for all the studied parameters except leaf sugar contents. Cultivar Abasin-95 superseded rest of the cultivars in leaf proline and sugar contents while cultivar Dur e Nifa and cultivar Nifa Gold showed maximum leaf carotenoid and phenol contents respectively. The high production of such stress combating metabolites indicate that these canola cultivars possess strong genes which can cope with adverse environment and is suggested for cultivation as an alternate crop in plant challenging conditions.

VIEWS 20

Abdel HMA, Mohammed HI, Mohamed LM, Zaki AM, Mogazy. 2012. Pre-exposure to gamma rays alleviates the harmful effect of salinity on cowpea plants. Journal of Stress Physiology & Biochemistry, 8(4), 199-217.

Altemimi A, Lakhssassi N, Baharlouei A, Watson DG, Lightfoot DA. 2017. Phytochemicals: Extraction, isolation, and identification of bioactive compounds from plant extracts. Plants 6(42), 1-23.

Ananthaswamy HM, Vakil V, Srinivasan A. 1971. Biochemical and physiological changes in gamma irradiated wheat during germination. Radiation Botany 11, 1-2.

Aremu SO, Nweze CC. 2017. Determination of vitamin A content from selected Nigerian fruits using spectropho- tometric method. Bangladesh Journal of Science and Industrial Research 52(2), 153-158.

Arvayo-Enríquez H, Mondaca-Fernández I, Gortárez-Moroyoqui P, López-Cervantes J, Rodríguez-Ramírez R. 2013. Carotenoids extraction and quantification: a review. Analytical Methods 5, 2916-2924.

Bassi R, Sharma SS. 1993. Changes in proline content accompanying the uptake of zinc and copper by Lemna minor. Ann Bot (Lond); 72, 151-154.

Bates L, Waldren RP, Teare ID. 1973. Rapid determination of free proline for water stress studies. Plant and Soil 39, 205-207.

Dubois M, Gills K, Hamilton J, Rebers P, Smith F. 1956. Colorimetric method for determination of sugars and related substances. Analytical Chemistry 28(3), 350-356.

Hare PD, Cress WA. 1997. Metabolic implications of stress-induced proline accumulation in plants. Plant Growth Regulation, 21, 79-102.

Hare PD, Cress WA, Staden JV. 1998. Dissecting the roles of osmolyte accumulation during stress. Plant Cell & Environment 21, 535-553.

Hayat S, Hayat Q, Alyemeni MN, Wani AS, Pichtel J, Ahmad A. 2012. Role of proline under changing environments Areview. Plant Signaling & Behavior 7(11), 1456–1466.

Jafri IF, Khan AH, Gulfishan M. 2011. Genotoxic effects of 5-bromouracil on cytomorphological characters of Cichoriumintybus L. African Journal of Biotechnology, 10(52), 10595-10599.

Jahangir M, Kim HK, Choi YH, Verpoorte R. 2009. Health-affecting compounds in Brassicaceae. Comprehensive Reviews Food Science and Food Safety 8, 31-43.

Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. 1951. Protein measurement with the folin phenol reagent. Journal of biological Chemistry, 193(1), 265-275.

Mahadevan A, Sridhar R. 1986. Methods in Physiological Plant Pathology. 3rd Edn. Sivakami Publication, Madras. India p 316.

Mezzoug N, Abrini J, Serano AM, Alonso-Moraga A, Idaomar M. 2006. Study on antigenotoxic effects of Moroccan medicinal plants and spices using the white/white + somatic assay in Drosophila. African Journal of Traditional and Complementry and Alternative Medicine 3, 22-31.

Mostafa GG. 2015. Effect of some chemical mutagens on the growth, phytochemical composition and induction of mutations in Khaya senegalensis. International Journal of Plant Breeding and Genetics, 9(2), 57-67.

Munns R. 2005. Genes and salt tolerance: bringing them together. New Phytologist 167, 645-63.

Naidu BP, Paleg LG, Aspinall D, Jennings AC, Jones GP. 1991. Amino acid and glycine betaine accumulation in cold- stressed wheat seedlings. Phytochemistry 30, 407-409.

Podsedek A. 2007. Natural antioxidants and antioxidant capacity of Brassica vegetables: A review. Lwt-Food Science and Technology 40, 1-11.

Rhodes D, Nadolska-Orczyk A, Rich PJ. 2002. Salinity, osmolytes and compatible solutes in: Lauchli A, Luttge U, eds. Salinity, Environment, Plant, Molecules. Netherlands: Al-Kluwer Academic Publishers, 181-204.

Sharma SS, Dietz KJ. 2006. The significance of amino acids and amino acid-derived molecules in plant responses and adaptation to heavy metal stress. Journal of Experimental Botany 57, 711-726.

Shrivastava P, Tiwari HN. 2008. Deleterious effects of chemical mutagens on economically important plant Hibiscus sabdariffa Var. Amv1 Linn. Journal of Environmental Research and Development 2(4), 633.

Steel RGD, Torrie JH. 1984. Principles and procedures of Statistics. A biometrical approach. 2ndEd. McGraw Hill Book, Co. Inc.New York.