Welcome to International Network for Natural Sciences | INNSpub

Exploring the role of tiny, potent player microRNAs in tea plant (Camellia sinensis) under the influence of pathogen attacks: Review

Research Paper | March 1, 2022

| Download 34

Samina Kausar, Rana Badar Aziz, Muhammad Shahbaz, Muhammad Adeel Ghani, Abdullah bin shaikh, Muhammad Shah Nawaz, Mansoor Hameed, Muhammad Zia Shahid, Asad Nawab, Muhammad Usman Shoukat

Key Words:

Int. J. Biosci.20( 3), 37-51, March 2022

DOI: http://dx.doi.org/10.12692/ijb/20.3.37-51


IJB 2022 [Generate Certificate]


Tea is cultivated as a cash crop all over the world and is consumed by more than 2 billion people throughout 125 countries nowadays. It is a nutrient-dense beverage with therapeutic characteristics that has a long list of health advantages. Pathogenic attacks are becoming the main dilemma in tea plants because of the continuous and adventurous development of plant pathogens, including insect pests, fungi, bacteria, viruses. Biogenesis pathways of miRNA are vital for the development, improvement, and protection of tea plants. MiRNAs of plants play key roles in a variety of regulating networks relating to plant growth, metabolic signaling, and environmental stress reactions. Various techniques, i.e., Transcriptional gene silencing, virus-induced gene silencing, Hairpin gene silencing, co-suppression, and artificial miRNAs, all these techniques are RNA interference (RNAi) strategies that have been used to shield tea cultivars from various stressed conditions. In this review paper, we summarized recent findings of miRNA-mediated regulation, features, and the defensive system of tea cultivar’s responses to plant pathogens, particularly fungal pathogenic attacks and insect herbivory. Eventually, we concluded the novel functions of miRNA-mediated gene silencing for future research and how it can be utilized for the improvement of pathogenic attacks tolerance in transgenic tea plants.


Copyright © 2022
By Authors and International Network for
Natural Sciences (INNSPUB)
This article is published under the terms of the Creative
Commons Attribution Liscense 4.0

Exploring the role of tiny, potent player microRNAs in tea plant (Camellia sinensis) under the influence of pathogen attacks: Review

Akmal M, Baig MS, Khan JA. 2017. Suppression of cotton leaf curl disease symptoms in Gossypium hirsutum through over expression of host-encoded miRNAs. Journal of Biotechnology 263, 21–29.

Arimura GI, Matsui K, Takabayashi J. 2009. Chemical and molecular ecology of herbivore-induced plant volatiles: proximate factors and their ultimate functions. Plant Cell Physiology 50, 911–923.

Artico S, Ribeiro-Alves M, Oliveira-Neto OB. 2014. Transcriptome analysis of Gossypium hirsutum fower buds infested by cotton boll weevil (Anthonomus grandis) larvae. BMC Genomics 15, 854.

Bartels D, Sunkar R. 2005. Drought and salt tolerance in plants. Critical Reviews in Plant Sciences 24, 23–58.

Berrocal-Lobo M, Molina A, Solano R. 2002. Constitutive expression of ETHYLENE- RESPONSE-FACTOR1 in arabidopsis confers resistance to several necrotrophic fungi. The Plant Journal 29, 23–32.

Borchetia S, Handique G, Roy S. 2018. Genomics approaches for biotic and abiotic stress improvement in tea. In: Han WY, Li X, Ahammed G. editors. Stress  physiology of tea in the face of climate change. Singapore: 13, 289–312.

Boros K, Jedlinszki N, Csupor D. 2016. Theanine and caffeine content of infusions prepared from commercial tea samples. Pharmacognosy Magazine 12(45), 75–79.

Campo S, Peris-Peris C, Sire C. 2013. Identification of a novel microRNA (miRNA) from rice that targets an alternatively spliced transcript of the Nramp6 (Natural resistance-associated macrophage protein 6) gene involved in pathogen resistance. New Philologist 199, 212–227.

Chacko SM, Thambi PT, Kuttan R. 2010. Beneficial effects of green tea: a literature review. Chinese Medicine 5, 13.

Chen L, Apostolides Z, Chen ZM. 2012. Global tea breeding: achievements, challenges and perspectives. Hangzhou, China: Springer-Zhejiang University Press.

Chen X. 2010. Small RNAs – secrets and surprises of the genome. The Plant Journal 61(6), 941–958.

Chen YF, Etheridge N, Schaller GE. 2005. Ethylene signal transduction. Annals of Botany 95, 901–915.

Chen YG, Huang MD. 2001. Biological control progress of Ectropis oblique Prout. Environmental Entomology 4, 181–184.

Chen ZM, Sun XL, Dong WX. 2012. Genetics and chemistry of the resistance of tea plant to pests. In: Chen L, Apostolides Z, Chen Z-M, editors. Advanced topics in Science and Technology in China. Global Tea Breeding. Berlin, Heidelberg: Springer; 343–360.

Curaba J, Talbot M, Li Z. 2013. Over-expression of microRNA171 affects phase transitions and floral meristem determinancy in barley. BMC Plant Biology 13, 6.

Das A, Pramanik K, Sharma R. 2019. In-silico study of biotic and abiotic stress-related transcription factor binding sites in the promoter regions of rice germin-like protein genes. Public Library of Science 14(2), e0211887.

Ding Q, Zeng J, He XQ. 2016. MiR169 and its target PagHAP2-6 regulated by ABA are involved in poplar cambium dormancy. Journal of Plant Physiology 198, 1–9.

Fang Y, Spector DL. 2007. Identification of nuclear dicing bodies containing proteins for microRNA biogenesis in living arabidopsis plants. Current Biology 17, 818–823.

Gao P, Bai X, Yang L. 2010 Over-expression of osa-MIR396c decreases salt and alkali stress tolerance. Planta 231(5), 991–1001.

Guo Y, Zhao S, Zhu C. 2017. Identification of drought-responsive miRNAs and physiological characterization of tea plant (Camellia sinensis L.) under drought stress. BMC Plant Biology 17(1), 211.

Hanley ME, Lamont BB, Fairbanks MM. 2007. Plant structural traits and their role in antiherbivore defense. Perspectives in Plant Ecology, Evolution and Systematics 8, 157–178.

Hayat K, Iqbal H, Malik U, Bilal U, Mushtaq S. 2015. Tea and its consumption:benefits and risks. Critical Reviews in Food Science and Nutrition 55(7), 939-954.

Hill MG, Wurms KV, Davy MW. 2015. Transcriptome analysis of kiwifruit (Actinidia chinensis) bark in response to armoured scale insect (Hemiberlesia lataniae) feeding. Public Library of Science 10, e0141664.

Huang GT, Ma SL, Bai LP. 2012. Signal transduction during cold, salt, and drought stresses in plants. Molecular Biololgy Reports 39, 969–987.

Jeyaraj A, Liu S, Zhang X. 2017b. Genome-wide identification of microRNAs responsive to Ectropis oblique feeding in tea plant (Camellia sinensis L.). Scientific Reports 7(1), 13634.

Jeyaraj A, Wang X, Wang S. 2019. Identification of regulatory networks of microRNAs and their targets in response to Colletotrichum gloeosporioides in tea plant (Camellia sinensis L.). Frontiers in Plant Science 10, 1096.

Jeyaraj A, Zhang X, Hou Y. 2017a. Genome-wide identification of conserved and novel microRNAs in one bud and two tender leaves of tea plant (Camellia sinensis) by small RNA sequencing, microarray-based hybridization and genome survey scaffold sequences. Bmc Plant Biology 17(1), 212.

Jeyaraj Anburaj, Tamilselvi E, Xinghui Li Guiyi G. 2020. Utilization of microRNAs and their regulatory functions for improving biotic stress tolerance in tea plant [Camellia sinensis (L.) O. Kuntze]. RNA Biology 17(10), 1365–1382.

Jones-Rhoades MW, Bartel DP, Bartel B. 2006. MicroR3and their regulatory roles in plants. Annual Review of Plant Biology 57, 19–53.

Khalid A, Zhang Q, Yasir M. 2017. Small RNA based genetic engineering for plant viral resistance: application in crop protection. Frontiers in Microbiology 8, 43.

Khan N, Mukhtar H. 2013. Tea and health: studies in humans. Current Pharmaceutical Design (7), 6141-6147.

Khraiwesh B, Zhu JK, Zhu J. 2012. Role of miRNAs and siRNAs in biotic and abiotic stress responses of plants. Biochimica et Biophysica Acta 1819(2), 137–148.

Kiep V, Vadassery J, Lattke J. 2015. Systemic cytosolic Ca2+ elevation is activated upon wounding and herbivory in Arabidopsis. New Phytologist 207, 996–1004.

Kuhns EH, Seidl-Adams I, Tumlinson JH. 2012. Heliothine caterpillars differ in abundance of a gut lumen aminoacylase (-ACY-1)- suggesting a relationship between host preference and fatty acid amino acid conjugate metabolism. Journal of Insect Physiology 58, 408–412.

Kumar R. 2014. Role of microRNAs in biotic and abiotic stress responses in crop plants. Applied Biochemistry and Biotechnology 174, 93–115.

Kurihara Y, Takashi Y, Watanabe Y. 2006. The interaction between DCL1 and HYL1 is important for efficient and precise processing of pri-miRNA in plant microRNA biogenesis. RNA 12, 206–212.

Li J, Yang Z, Yu B. 2005. Methylation protects miRNAs and siRNAs from a 3ʹ-end uridylation activity in arabidopsis. Current Biology 15, 1501–1507.

Li Y, Zhang QQ, Zhang J. 2010. Identification of microRNAs involved in pathogen-associated molecular pattern-triggered plant innate immunity. Plant Physiology 152, 2222–2231.

Liu N, Tu L, Wang L. 2017. MicroRNA 157-targeted SPL genes regulate floral organ size and ovule production in cotton. BMC Plant Biology 17(1), 7.

Liu S, Liu H, Wu A, Hou Y, An Y, Wei C. 2017. Construction of fingerprinting for tea plant (Camellia sinensis) accessions using new genomic SSR markers. MolecularBreeding 37(8), 93. http://dx.doi.org/10.1007/s11032-017-0692-y.

Liu S, Mi X, Zhang R. 2019. Integrated analysis of miRNAs and their targets reveals that miR3Sc/TCP2 regulates apical bud burst in tea plant (Camellia sinensis). Planta. 250(4), 1111–1129.

Liu SC, Xu YX, Ma JQ. 2016. Small RNA and degradome profiling reveals important roles for microRNAs and their targets in tea plant response to  drought stress. Plant Physiology 158(4), 435–451.

Lu Y, Feng Z, Bian L. 2010. miR398 regulation in rice of the responses to abiotic and biotic stresses depends on CSD1 and CSD2 expression. Functional Plant Biology 38, 44–53.

Ma JQ, Jin JJ, Yao MZ, Ma CL, Xu YX, Hao WG, Chen L. 2018. Quantitative trait loci mapping for theobromine and caffeine contents in tea plant (Camellia sinensis). Journal of Agricultural and Food Chemistry 66(50), 13321-13327.

Ma Q, Chen C, Zeng Z, Zou Z, Li H, Zhou Q, Chen X, Sun K, Li X. 2018b. Transcrip-tomic analysis between self- and cross-pollinated pistils of tea plants (Camelliasinensis). BMC Genomics 19, 289. http://dx.doi.org/10.1186/s12864-018-4674-1.

Ma Z, He S, Wang X, Sun J, Zhang Y, Zhang G, Wu L, Li Z, Liu Z, Sun G. 2018c. Resequencing a core collection of upland cotton identifies genomic variation and loci influencing fiber quality and yield. Nature Genetics 50(6), 803-813.

Ma Z, Jiang J, Hu Z. 2017. Over-expression of miR158 causes pollen abortion in Brassica campestris ssp. Chinensis Plant Molecular Biology 93(3), 313–326.

Mishra R, Mohanty JN, Chand SK. 2018. Can-miRn37a mediated suppression of ethylene response factors enhances the resistance of chilli against anthracnose pathogen Colletotrichum truncatum L. Plant Science 267, 135–147.

Naghma K, Hasan M. 2013. Tea and health: studies in humans. Current Pharmaceutical Design 19(34), 6141-6147 http://dx.doi.org/10.2174/1381612811319340008.

Nakata M, Mitsuda N, Herde M. 2013. A bHLH-type transcription factor, ABA-INDUCIBLE BHLH-TYPE TRANSCRIPTION FACTOR/JA-ASSOCIATED MYC2-LIKE1, acts as a repressor to negatively regulate jasmonate signaling in arabidopsis. Plant Cell 25, 1641–1656.

Navarro L, Dunoyer P, Jay F. 2006. A plant miRNA contributes to antibacterial resistance by repressing auxin signalling. Science 12, 436–439.

Naya L, Paul S, Valdés-López O. 2014.Regulation of copper homeostasis and biotic interactions by microRNA 398b in common bean. Public Library of Science 9(1), e84416.

Niu J, Wang J, Hu H. 2016. Cross-talk between freezing response and signaling for regulatory transcriptions of MIR475b and its targets by miR475b promoter in Populus suaveolens. International Journal of Scientific Reports 6, 1–11.

Niu Suzhen, Hisashi K, Qinfei S, Dahe Q, Juan C, Degang Z, Zhengwu C, Ying W, Tianyuan Z. 2020. Development of core-collections for Guizhou tea genetic resources and GWAS of leaf size using SNP developed by genotyping-by-sequencing. Peer Journal 13(8), e8572. http://dx.doi.org/10.7717/peerj.8572

Nurnberger T, Kemmerling B. 2006. Receptor protein kinases-pattern recognition receptors in plant immunity. Trends in Plant Science 11, 519–522.

Pavani A, Chaitanya RK, Chauhan VK. 2015. Differential oxidative stress responses in castor semilooper. Achaea janata. Journal of Invertebrate Pathology 132, 157–164.

Ponmurugan P, Baby UI, Rajkumar R. 2007. Growth, photosynthetic and biochemical responses of tea cultivars infected with various diseases. Photosynthesis 45, 143–146.

Ponmurugan P, Manjukarunambika K, Gnanamangai BM. 2016. Impact of various foliar diseases on the biochemical, volatile and quality constituents of green and black teas. Australasian Plant Pathology 45, 175.

Prabu GR, Mandal AK. 2010. Computational identification of miRNAs and their target genes from expressed sequence tags of tea (Camellia sinensis). Genomics Proteomics Bioinformatics 8 (2), 113–121.

Qi T, Guo J, Peng H. 2019. Host-induced gene silencing: a powerful strategy to control diseases of wheat and barley. International journal of Molecular Science 20(1), pii: E206.

Saifi M, Yogindran S, Nasrullah N. 2019. Co-expression of anti-miR319g and miRStv_11 lead to enhanced steviol glycosides content in stevia rebaudiana. BMC Plant Biology 19(1), 274.

Sasaki-Sekimoto Y, Jikumaru Y, Obayashi T. 2013. Basic helix-loop-helix transcription factors JASMONATE-ASSOCIATED MYC2-LIKE1 (JAM1), JAM2, and JAM3 are negative regulators of jasmonate responses in Arabidopsis. Plant Physiology. 163, 291–304.

Shi ZH, Zhang C, Xu XF.  2015. Overexpression of AtTTP affects ARF17 expression and leads to male sterility in arabidopsis. Public liberty of science 10(3), e0117317.

Song JB, Gao S, Sun D. 2013. miR394 and LCR are involved in arabidopsis salt and drought stress responses in an abscisic acid-dependent manner. BMC Plant Biology 13, 210.

Sun G. 2012. MicroRNAs and their diverse functions in plants. Plant Molecular Biology. 80, 17–36.

Sunkar R, Li YF, Jagadeeswaran G. 2012. Functions of microRNAs in plant stress responses. Trends of Plant Science 17, 196–203.

Tai Y, Liu C, Yu S, Yang H, Sun J, Guo C, Huang B, Liu Z, Yuan Y, Xia E, Wei C, Wan X. 2018. Gene co-expression network analysis reveals coordinated regulation of three characteristic secondary biosynthetic pathways in tea plant (Camellia sinensis). BMC Genomics 616.

Tang Y, Liu H, Guo S. 2018. OsmiR396d affects gibberellin and brassinosteroid signaling to regulate plant architecture in rice. Plant Physiology 176(1), 946–959.

Teotia S, Zhang D, Tang G. 2017. Knockdown of rice microRNA166 by short tandem target mimic (STTM). Methods in Molecular Biology 1654, 337–349.

Tewari A. 2016.To check the bio-efficiency of deuce 2.8 (deltamethrin) against Helopeltis theivora or more commonly known as the tea mosquito. International Journal of Applied Science and Engineering 2, 31–35.

Tiwari M, Sharma D, Trivedi PK. 2014. Artificial microRNA mediated gene silencing in plants: progress and perspectives. Plant Molecular Biology 86, 1–18.

Unver T, Namuth-Covert DM, Budak H. 2009. Review of current methodological approaches for characterizing microRNAs in plants. International journal of Plant Genomics. 262463.

Voinnet O .2009. Ori gin, biogenesis, and activity of plant microRNAs. Cell 136,669–687.

Wambulwa MC, Meegahakumbura MK, Kamunya S, Muchugi A, Moller M, Liu J, Xu Wang M, Jin H. 2017. Spray-induced gene silencing: a powerful innovative strategy for crop protection. Trends in Microbiology 25(1), 4–6.

Wambulwa MC, Meegahakumbura MK, Kamunya S, Muchugi A, Moller M, Liu J, Xu JC, Ranjitkar S, Li DZ, Gao LM. 2016. Insights into the genetic relationships and breeding patterns of the African tea germplasm based on nSSR markers and cpDNA sequences. Frontiers in Plant Science 7, 1244. http://dx.doi.org/10.3389/fpls.2016.01244.

Wang T, Sun MY, Wang XS. 2016. Over-expression of GmGIa-regulated soybean miR172a confers early flowering in transgenic arabidopsis thaliana. International Journal of Molecular Science 17(5), pii: E645.

Wang Y, Wang Z, Yang W. 2019. Degradation of fungal microRNAs triggered by short tandem target mimics is via the small-RNA-degrading nuclease. Applied and Environmental Microbiology Journal 85, 9.

Wang YN, Tang L, Hou Y. 2016. Differential transcriptome analysis of leaves of tea plant (Camellia sinensis) provides comprehensive insights into the defense responses to Ectropis oblique attack using RNA-Seq. Functional and Integrative Genomics 16, 383–398.

Waugh DT, Godfrey M, Limeback H. 2017. Black tea source, production, and consumption: assessment of health risks of fluoride intake in New Zealand. Journal of Environmental and Public Health 5120504.

Wei C, Yang H, Wang S, Zhao J, Liu C, Gao L, Xia E, Lu. 2018. Draft genome sequence of Camellia sinensis var. sinensis provides insights into he evolution of the tea genome and tea quality. Proceedings of the National Academy of Sciences of the United States of America 115(18), E4151-E4158.

Xia EH, Zhang HB, Sheng J, Li K, Zhang QJ, Kim C, Zhang Y, Liu. 2017. The tea tree genome provides insights into tea flavor and independent evolution of caffeine biosynthesis. Molecular Plant 10(6), 866-877.

Xia EH, Zhang HB, Sheng J. 2017. The tea tree genome provides insights into tea flavor and independent evolution of caffeine biosynthesis. Molecular Plant 10(6), 866–877.

Xia EH, Zhang HB, Sheng J, Li K, Zhang QJ, Kim C, Zhang. 2017. The tea tree genome provides insights into tea flavor and independent evolution of caffeine biosynthesis Molecular Plant 10, 866–877.

Xu W, Dubos C, Lepiniec L. 2015. Transcriptional control of flavonoid biosynthesis by MYB-bHLH-WDR complexes. Trends of Plant Sciemce 20, 176–185.

Yang C, Li D, Mao D. 2013. Over-expression of microRNA319 impacts leaf morphogenesis and leads to enhanced cold tolerance in rice (Oryza sativa L.). Plant Cell Environment 36, 2207–2218.

Yang CS, Landau JM. 2000. Effects of tea consumption on nutrition and health. The Journal of Nutrition 130(10), 2409–2412.

Yang Z, Ebright YW, Yu B. 2006. HEN1 recognizes 21–24 nt small RNA duplexes and deposits a methyl group onto the 2ʹ OH of the 3ʹ terminal nucleotide. Nucleic Acids Research 34,667–675.

Yao JL, Tomes S, Xu J. 2017. How microRNA172 affects fruit growth in different species is dependent on fruit type. Plant Signal Behav. 11(4), e1156833.

Yogindran S, Ghosh A, Rajam MV. 2015. Artificial miRNAs for specific gene silencing and engineering virus resistance in plants. Frontiers in Cell and Developmental Biology 4, e137.

Zhang B, Wang Q. 2015 MicroRNA-based biotechnology for plant improvement. Journal of Cell Physiology 230, 1–15.

Zhang B, Wang Q. 2016. MicroRNA, a new target for engineering new crop varieties. Bioengineered 7, 7–10.

Zhang F, Wang L, Lim JY. 2016. Phosphorylation of CBP20 Links microRNA to root growth in the ethylene response. Public Library of Science 12(11), e1006437.

Zhang X, Zou Z, Gong P. 2011. Over-expression of microRNA169 confers enhanced drought tolerance to tomato. Biotechnology Letters 33(2), 403–409.

Zhang Y, Zhu X, Chen X. 2014. Identification and characterization of cold-responsive microRNAs in tea plant (Camellia sinensis) and their targets using high-throughput sequencing and degradome analysis. BMC Plant Biology 14,271.

Zhang Z, Feng X, Wang Y. 2019. Advances in research on functional genes of tea plant. Gene. 711, 143940

Zhang S, Zhang L, Tai Y, Wang X, Ho CT, Wan X. 2018. Gene discovery of characteristic metabolic pathways in the tea plant (Camellia sinensis) using’ Omics’-Based network approaches: a future perspective. Frontiers of Plant Science 9, 480.

Zhao L, Chen C, Wang Y. 2019. Conserved microRNA act boldly during sprout development and quality formation in Pingyang Tezaocha (Camellia sinensis). Frontiers in Genetics 10, 237.

Zheng C, Zhao L, Wang Y. 2015. Integrated RNA-Seq and sRNA-Seq analysis identifies chilling and freezing responsive key molecular players and pathways in tea plant (Camellia sinensis). Public Library of Science 10(4), e0125031.

Zheng LL, Qu LH. 2015. Application of microRNA gene resources in the improvement of agronomic traits in rice. Plant Biotechnology Journal 13(3), 329–336.

Zhou M, Luo H. 2013. MicroRNA-mediated gene regulation: potential application for plant genetic engineering. Plant Molecular Biology 83, 59–75.

Zhou Y, Liu Y, Wang S. 2017. Molecular cloning and characterization of galactinol synthases in Camellia sinensis with different responses to biotic and abiotic stressors. Journal of Agriculture Food Chemistry 65(13), 2751–2759.

Zhu QH, Upadhyaya NM, Gubler F. 2009. Over-expression of miR172 causes loss of spikelet determinacy and floral organ abnormalities in rice (Oryza sativa). BMC Plant Biology 9, 149.

Zhu QW, Luo YP. 2013. Identification of miRNAs and their targets in tea (Camellia sinensis). Journal of Zheijang University science b 14(10), 916–923.