Exploring the therapeutic efficacy of plga-ndp: A comprehensive analysis in oecm-1 oral cancer cells
Paper Details
Exploring the therapeutic efficacy of plga-ndp: A comprehensive analysis in oecm-1 oral cancer cells
Abstract
The study investigates therapeutic potential of PLGA-NDP in OECM-1 oral cancer cells. PLGA-NDP presented higher cytotoxicity, lower redox reaction, and higher release activity in relation to nedaplatin alone. With PLGA-NDP treatment the colony formation assay exhibited a substantial minimize in cell survival. PLGA-NDP also moderate MMP-9 release, indicating inhibition of cellular degranulation. The dose-dependent responses and increased efficacy in relation to nedaplatin alone recommend the potential of PLGA-NDP as novel therapy for oral cancer. These results advise that PLGA-NDP nanoparticles may be more efficient alternative to conventional nedaplatin therapy in cancer treatment, which may result in higher efficiency and decline adverse effects.
Alkahtani S, Alarifi S, Albasher G, Al-Zharani M, Aljarba NH, Almarzoug MH. 2021. Poly lactic‐co‐glycolic acid‐ (PLGA‐) loaded nanoformulation of cisplatin as a therapeutic approach for breast cancers. Oxidative Medicine and Cellular Longevity 2021, 5834418. https://onlinelibrary.wiley.com/doi/10.1155/2021/5834418
Annamalai G, Kathiresan S, Kannappan N. 2016. [6]-Shogaol, a dietary phenolic compound, induces oxidative stress mediated mitochondrial-dependent apoptosis through activation of proapoptotic factors in Hep-2 cells. Biomedicine & Pharmacotherapy 82, 226–236. https://linkinghub.elsevier.com/retrieve/pii/S0753332216302013
Annamalai G, Kathiresan S, Kannappan N. 2016. [6]-Shogaol, a dietary phenolic compound, induces oxidative stress mediated mitochondrial-dependent apoptosis through activation of proapoptotic factors in Hep-2 cells. Biomedicine & Pharmacotherapy 82, 226–236. https://linkinghub.elsevier.com/retrieve/pii/S0753332216302013
Bahuguna A, Khan I, Bajpai VK, Kang SC. 2017. MTT assay to evaluate the cytotoxic potential of a drug. Bangladesh Journal of Pharmacology 12(2). https://www.banglajol.info/index.php/BJP/article/view/30892
Carreño EA, Alberto AVP, De Souza CAM, De Mello HL, Henriques-Pons A, Anastacio Alves L. 2021. Considerations and technical pitfalls in the employment of the MTT assay to evaluate photosensitizers for photodynamic therapy. Applied Sciences 11(6), 2603. https://www.mdpi.com/2076-3417/11/6/2603
Chen H, Zou Y, Yang H, Wang J, Pan H. 2014. Downregulation of FoxM1 inhibits proliferation, invasion and angiogenesis of HeLa cells in vitro and in vivo. International Journal of Oncology 45(6), 2355–2364.https://www.spandidos-publications.com/10.3892/ijo.2014.2645
Chenni SI, Suresh DK, Venkatesan DP, Kannappan N, Rajasekar DM, Ahamed AAN. 2025. Neoterics preparation of PLGA loaded nedaplatin nanoparticles by various data analysis. International Journal of Advanced Research in Science and Technology 15(02).
Fang J, Zhao X, Li S, Xing X, Wang H, Lazarovici P. 2019. Protective mechanism of artemisinin on rat bone marrow-derived mesenchymal stem cells against apoptosis induced by hydrogen peroxide via activation of c-Raf-Erk1/2-p90rsk-CREB pathway. Stem Cell Research & Therapy 10(1), 312. https://stemcellres.biomedcentral.com/articles/10.1186/s13287-019-1419-2
Fukuishi N, Murakami S, Ohno A, Yamanaka N, Matsui N, Fukutsuji K. 2014. Does β-hexosaminidase function only as a degranulation indicator in mast cells? The primary role of β-hexosaminidase in mast cell granules. Journal of Immunology 193(4), 1886–1894. https://academic.oup.com/jimmunol/article/193/4/1886/7960371
George A, Ranganathan K, Rao UK. 2011. Expression of MMP-1 in histopathological different grades of oral squamous cell carcinoma and in normal buccal mucosa – an immunohistochemical study. Cancer Biomarkers 7(6), 275–283. https://journals.sagepub.com/doi/full/10.3233/CBM-2010-0191
Givony S. 2020. Oral squamous cell carcinoma (OSCC): An overview. 8(13).
Gong Y, Chippada-Venkata U, Oh W. 2014. Roles of matrix metalloproteinases and their natural inhibitors in prostate cancer progression. Cancers 6(3), 1298–1327. https://www.mdpi.com/2072-6694/6/3/1298
Huang XF, Chang KF, Lee SC, Li CY, Liao HH, Hsieh MC. 2020. Extract of Juniperus indica Bertol synergizes with cisplatin to inhibit oral cancer cell growth via repression of cell cycle progression and activation of the caspase cascade. Molecules 25(12), 2746. https://www.mdpi.com/1420-3049/25/12/2746
Ilanchit Chenni S, Suresh K, Theerthu A, Ahamed AAN, Pugazhendhi R, Vasu R. 2024. PLGA‐loaded nedaplatin (PLGA‐NDP) inhibits 7,12‐dimethylbenz[a]anthracene (DMBA) induced oral carcinogenesis via modulating Notch signaling pathway and induces apoptosis in experimental hamster model. Cell Biochemistry and Function 42(7), e4133. https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/cbf.4133
Lu S, Chen Z, Hu C, Zhang J, Chen Y, Song Y. 2018. Nedaplatin plus docetaxel versus cisplatin plus docetaxel as first-line chemotherapy for advanced squamous cell carcinoma of the lung — a multicenter, open-label, randomized, phase III trial. Journal of Thoracic Oncology 13(11), 1743–1749. https://linkinghub.elsevier.com/retrieve/pii/S1556086418307822
Majtnerova P, Capek J, Petira F, Handl J, Rousar T. 2021. Quantitative spectrofluorometric assay detecting nuclear condensation and fragmentation in intact cells. Scientific Reports 11(1), 11921. https://www.nature.com/articles/s41598-021-91380-3
Mandelkow R, Gümbel D, Ahrend H, Kaul A, Zimmermann U, Burchardt M. 2017. Detection and quantification of nuclear morphology changes in apoptotic cells by fluorescence microscopy and subsequent analysis of visualized fluorescent signals. Anticancer Research 37(5), 2239–2244. http://ar.iiarjournals.org/content/37/5/2239.abstract
Moreno D, Zalba S, Navarro I, Tros De Ilarduya C, Garrido MJ. 2010. Pharmacodynamics of cisplatin-loaded PLGA nanoparticles administered to tumor-bearing mice. European Journal of Pharmaceutics and Biopharmaceutics 74(2), 265–274. https://linkinghub.elsevier.com/retrieve/pii/S0939641109003257
Rajendran V, Jain MV. 2018. In vitro tumorigenic assay: Colony forming assay for cancer stem cells. In: Papaccio G, Desiderio V, editors. Cancer Stem Cells. Methods in Molecular Biology 1692, 89–95. http://link.springer.com/10.1007/978-1-4939-7401-6_8
Ramadoss H, Kathiresan S, Kathiresan S. 2021. Anti-proliferative activity of aescin on human oral carcinoma (KB) cells and human laryngeal carcinoma (Hep-2) cells via cell sensitivity assays. 25(2).
Sathiya Kamatchi T, Mohamed Subarkhan MK, Ramesh R, Wang H, Małecki JG. 2020. Investigation into antiproliferative activity and apoptosis mechanism of new arene Ru(II) carbazole-based hydrazone complexes. Dalton Transactions 49(32), 11385–11395. https://xlink.rsc.org/?DOI=D0DT01476A
Shoari A, Ashja Ardalan A, Dimesa AM, Coban MA. 2024. Targeting invasion: The role of MMP-2 and MMP-9 inhibition in colorectal cancer therapy. Biomolecules 15(1), 35. https://www.mdpi.com/2218-273X/15/1/35
Song N, Wang B, Feng G, Duan L, Yuan S, Jia W. 2019. Knockdown of high mobility group box 3 impairs cell viability and colony formation but increases apoptosis in A549 human non‑small cell lung cancer cells. Oncology Letters. http://www.spandidos-publications.com/10.3892/ol.2019.9927
Strober W. 2015. Trypan blue exclusion test of cell viability. Current Protocols in Immunology 111(1). https://currentprotocols.onlinelibrary.wiley.com/doi/10.1002/0471142735.ima03bs111
Takahashi K, Fukushima K, Otagaki S, Ishimoto K, Minami K, Fukushima N. 2018. Effects of LPA1 and LPA6 on the regulation of colony formation activity in colon cancer cells treated with anticancer drugs. Journal of Receptors and Signal Transduction 38(1), 71–75. https://www.tandfonline.com/doi/full/10.1080/10799893.2018.1426608
Senkuttuvan ILanchit Chenni, Kathiresan Suresh, Azhamuthu Theerthu, Abulkalam Asath Nihal Ahamed, Ravichandran Pugazhendhi, Rajeswari Vasu (2025), Exploring the therapeutic efficacy of plga-ndp: A comprehensive analysis in oecm-1 oral cancer cells; IJB, V26, N4, April, P198-207
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