Expression of polymorphic profiles of specific and nonspecific esterase in multivoltine and bivoltine silkworm, Bombyx mori L.
Paper Details
Expression of polymorphic profiles of specific and nonspecific esterase in multivoltine and bivoltine silkworm, Bombyx mori L.
Abstract
An approach has been made to understand the expression of the specific and nonspecific esterase polymorphism in whole body tissue of the silkworm breeds namely, CSR2, CSR4, Pure Mysore and C.nichi. utilizing a polyacrylamide gel stained with α- and β-naphthylacetate as nonspecific hydrolyzing substrate. The genetic diversity among silkworm breeds existed in relation to isozyme of nine esterases, but most of the esterase profiles, such as, Est-4, 5, 6, 7, 8, and 9 found to be noticed as nonspecific utilizing two substrates. The Est-1 is confirmed to be a specific α -esterase in the silkworm as it appears with α -naphthylacetate in CSR2 and CSR4 bivoltine breeds. The Est-2 observed to be nonspecific for all selected breeds except C.nichi, whereas, Est-3 was nonspecific for bivoltine breeds but specific for mutivoltine races. The expression of the isozymes Est-5 and Est-9 are revealed as breed-specific esterases as represented their profiles only in Pure Mysore and CSR4 breed respectively. The outcome of the study interprets the origin of breeds substantiated the significant role of isoenzyme marker for analysis of gene variation between the species in relation to expression of the voltinism in geographically isolated silkworms.
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