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Extended spectrum beta lactamases producing non-lactose fermentative bacterial isolates causing blood stream infections in children

Research Paper | August 1, 2019

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Anees Muhammad, Muhammad Jaseem Khan, Inam Ullah, Habib Ullah Khan, Ihteshamul Haq

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Int. J. Biosci.15( 2), 61-69, August 2019

DOI: http://dx.doi.org/10.12692/ijb/15.2.61-69


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Blood stream infections (BSIs) are the important cause of morbidity and mortality in pediatrics. BSIs are usually caused by common gram positive and gram negative bacterial isolates but few uncommon bacteria may lead to BSIs in children significantly. The aim of the present study was to determine the drug resistance pattern of uncommon non-lactose fermenting gram negative bacterial isolates from blood specimen of children. A cross sectional study was conducted at tertiary care hospital, Peshawar from June to December 2018. Blood specimens were collected aseptically in BACTAMTM bottles and were processed in BACTEC 9120 according to the standard protocol. Antibiotics resistance profile was determined by using Kirby-Bauer Disc diffusion method. Bacterial isolates showed resistance to cephalosporin were further verified for extended spectrum beta lactamases (ESBL) by double disc diffusion method according to the clinical laboratory standards institute guidelines. Out of total, 20.6% were positive with significant growth in which 6.0% (07) isolates were non-lactose fermenter gram negative bacteria including Morgenella morganii (0.9%), Stenotrophomonas maltophila (2.7%), Acinetobacter baumannii (0.9) and Burkholderia cepacia (1.8%). Colistin/Polymixin B was found only effective antibiotics against Acinetobacter baumannii. Among recovered isolates, 42.9% were ESBL producer while 71.4 % were found multidrug resistant strains. It is concluded that non-fermenter bacterial isolates can contribute in blood stream infections significantly. ESBL producing by non-lactose fermenter bacterial isolates were identified with emerging MDR isolates to various antibiotics classes which is major concern in developing countries.


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Extended spectrum beta lactamases producing non-lactose fermentative bacterial isolates causing blood stream infections in children

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