Extraction and characterization of hydroxyapatite from bovine cortical bone and effect of radiation

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Research Paper 01/09/2017
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Extraction and characterization of hydroxyapatite from bovine cortical bone and effect of radiation

Md. Masud Rana, Naznin Akhtar, Md. Shaifur Rahman, Md. Zahid Hasan, S.M. Asaduzzaman
Int. J. Biosci.11( 3), 20-30, September 2017.
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The demands for the use of artificial materials in the biomedical application have increased significantly due to the limited accessibility of natural bone for grafting. To fulfill the demand, natural biomaterials play vital roles in the design and production of biocompatible matrix that is biomimetics, elucidating specific cell functions, allowing cell-cell interactions and tissue regeneration. Hydroxyapatite (HA) is a natural biomaterial, not only a biocompatible, osteoconductive, osteoinductive, non-toxic, non-inflammatory and non-immunogenic agent, but also bioactive andhas got the ability to form a direct chemical bond with living tissues and promote tissue growth. In this study, natural HA has been extracted from bovine cortical bones using a cost effective and ecofriendly way. Shortly, bovine cortical bones were annealed at different temperature ranging from 6500C to 12500C in an air atmosphere. The powder was characterized by thermo-gravimetric analysis (TGA), x-ray diffraction (XRD), flourier transformed infrared (FTIR) spectroscopy and scanning electron microscopy (SEM) analysis. The TGA result of raw bone indicated the presence of organic compounds which was removed upon annealing above 6500C. SEM analysis demonstrated that, the average particle size of extracted HA was 255.28 ±63 nm.XRD analysis confirmed that HA was the only crystalline phase when sintered at 9500C.Above this temperature HA was decomposed into β-TCP and α-TCP. After radiation the extracted HA, it was revealed that there were not any significant alteration of the properties of HA. It may be a good complement in different biomedical application.


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