home icon user icon
Welcome to International Network for Natural Sciences | INNS Pub.
Home My Account
Submit Manuscript
double_slash
breadcumb_bg

Extraction and characterization of hydroxyapatite from bovine cortical bone and effect of radiation

Paper Details

Research Paper 01/09/2017
Views (199) Download (146)
current_issue_feature_image
publication_file

Extraction and characterization of hydroxyapatite from bovine cortical bone and effect of radiation

Md. Masud Rana, Naznin Akhtar, Md. Shaifur Rahman, Md. Zahid Hasan, S.M. Asaduzzaman
Int. J. Biosci.11( 3), 20-30, September 2017.
Certificate: IJB 2017 [Generate Certificate]
Key: AnnealingBovine boneHydroxyapatiteRadiation

Abstract

The demands for the use of artificial materials in the biomedical application have increased significantly due to the limited accessibility of natural bone for grafting. To fulfill the demand, natural biomaterials play vital roles in the design and production of biocompatible matrix that is biomimetics, elucidating specific cell functions, allowing cell-cell interactions and tissue regeneration. Hydroxyapatite (HA) is a natural biomaterial, not only a biocompatible, osteoconductive, osteoinductive, non-toxic, non-inflammatory and non-immunogenic agent, but also bioactive andhas got the ability to form a direct chemical bond with living tissues and promote tissue growth. In this study, natural HA has been extracted from bovine cortical bones using a cost effective and ecofriendly way. Shortly, bovine cortical bones were annealed at different temperature ranging from 6500C to 12500C in an air atmosphere. The powder was characterized by thermo-gravimetric analysis (TGA), x-ray diffraction (XRD), flourier transformed infrared (FTIR) spectroscopy and scanning electron microscopy (SEM) analysis. The TGA result of raw bone indicated the presence of organic compounds which was removed upon annealing above 6500C. SEM analysis demonstrated that, the average particle size of extracted HA was 255.28 ±63 nm.XRD analysis confirmed that HA was the only crystalline phase when sintered at 9500C.Above this temperature HA was decomposed into β-TCP and α-TCP. After radiation the extracted HA, it was revealed that there were not any significant alteration of the properties of HA. It may be a good complement in different biomedical application.

VIEWS 8
Cover PDF

Akram M, Ahmed R, Shakir I, Aini W, Ibrahim W, Hussain R. 2014. Extracting  hydroxyapatite and its precursors from natural Resources. Journal of Materials Science, 49, 1461–1475.

Bahrololooma ME, Javidi M, Javadpoura S, Ma J. 2009. Characterisation of natural hydroxyapatite extracted from bovine cortical bone ash. Journal of Ceramic Processing Research, 10, 129–138.

Barakat NA, Khil MS, Omran A, Sheikh FA, Kim HY. 2009. Extraction of pure natural hydroxyapatite from the bovine bones bio waste by three different methods. Journal of Materials Processing Technology 209, 3408–3415.

Boskey A, Camacho NP. 2007. FT-IR imaging of native and tissue-engineered bone and cartilage. Biomaterials 28, 2465-2478,  .

Chattanathan S, Clement T, Kanel S, Barnett M, Chatakondi N. 2013. Remediation of uranium contaminated groundwater by sorption onto hydroxyapatite derived from catfish bones.Water Air and Soil Pollution. 224, 1–9.

Chattopadhyay P, Pal S,Wahi AK, Singh L, Verma A. 2007. Synthesis of Crystalline Hydroxyapatite from Coral (Gergonaceasp) and Cytotoxicity Evaluation.Trends in Biomaterials and Artificial Organs, 20(2), 142-144.

Davim JP, Marques N. 2004. Dynamical experimental study of friction and wear behaviour of bovine cancellous bone sliding against a metallic counter face in a water lubricated environment.Journal of Materials Processing Technology, 15, 124–128.

Destainville A, Champion E, Assollante DB, Laborde E. 2003. Synthesis, characterization and thermal behaviour of apatite tricalcium phosphate. Materials Chemistry and Physics 80, 269 – 277.

Dhandayuthapani B, Yoshida Y, Maekawa T, Kumar DS. 2011. Polymeric Scaffolds in Tissue Engineering Application: A Review.International Journal of Polymer Science, 2011, Article ID 290602.

Dimitriou R, Jones E, Mc Gonagle D, Giannoudis PV. 2011. Bone regeneration: current concepts and future directions. BMC Medicine 9, 66–76.

Fahmy MD, Jazayeri HE, Razavi M, Masri R, Tayebi L. 2016. Three-Dimensional Bioprinting Materials with Potential Application in Preprosthetic Surgery.Journal of Prosthodontics 25, 310–318.

Ferreira JRM, da Rocha DN, Louro LHL, da Silva MHP. 2014. Phosphating of Calcium Carbonate for Obtaining Hydroxyapatite from the Ostrich Egg Shell. Key Engineering Materials 587, 69-73.

Giedraitis A, Arnoczky SP, Bedi A. 2014. Allografts in Soft Tissue Reconstructive Procedures: Important Considerations.Sports Health, 6(3), 256-264.

Gomes S, Leonor IB, Mano JF, Reis RL, Kaplan DL. 2012. Natural and genetically engineered proteins for tissue engineering.Progress in Polymer Science, 37, 1–17.

Greenwald MA, Kuehnert MJ, Fishman JA. 2012. Infectious Disease Transmission during Organ and Tissue Transplantation.Emerging Infectious Diseases 18(8).

Haberko K, Bucko MM, Brzezinska-Miecznik J, Haberko M, Mozgawa W, Panz T, PydaA, Zarebski J. 2006. Natural hydroxyapatite—its behaviour during heat treatment. Journal of the European Ceramic Society 26, 537-542.

Han JK, Song HY, Saito F, Lee BT. 2006.Synthesis of height purity nano-sized hydroxyapatite powder by microwave-hydrothermal method.  Materials Chemistry and Physics 99, 235 – 239.

Jinlong N, Zhenxi Z, Dazong J. 2001. Investigation of phase evolution during the thermochemical synthesis of tri-calcium phosphate. Journal of Materials Synthesis and Processing 9, 235–240.

Joschek S, Nies B, Krotz R, Gofpferich A. 2000. Chemical and physicochemical characterization of porous hydroxyapatite ceramics made of natural bone. Biomaterials 21, 1645–1658.

Kim BS, Kang HJ, Yang SS, Lee J. 2014.Comparison of in vitro and in vivo bioactivity: Cuttlefish-bone-derived hydroxyapatite and synthetic hydroxyapatite granules as a bone graft substitute.  Biomedical Materials 9.

Kongsri S, Janpradit K, Buapa K, Techawongstien S, Chanthai S. 2013. Nanocrystalline hydroxyapatite from fish scale waste: Preparation, characterization and application for selenium adsorption in aqueous solution.Chemical Engineering Journal 215, 522–532.

Manalu JL, Soegijono B, Indrani DJ. 2015. Characterization of Hydroxyapatite Derived from Bovine Bone. Asian Journal of Applied Sciences 3(4).

Markovic M, Fowler BO, Tung MS. 2004. Preparation and comprehensive characterization of a calcium hydroxyapatite reference material. Journal of research of the National Institute of Standards and Technology109, 553-568.

Meejoo S, Maneeprakorn W, Winotai P. 2006. Phase and thermal stability of nanocrystalline hydroxyapatite prepared via microwave heating. Thermochimica Acta,  447, 115–120.

Miculescu F, Ciocan LT, Miculescu M, Ernuteanu A. 2011. Effect of heating process on micro structure level of cortical bone prepared for compositional analysis.Digest Journal of Nanomaterials and Biostructure, 6(1), 225 – 233.

Mobasherpour I, Heshajin M. 2007.Synthesis of nanocrystalline hydroxyapatite by using precipitation method. Journal of Alloys and Compounds, 430, 330 – 333.

Ooi CY, Hamdi M, Ramesh S. 2007. Properties of hydroxyapatite produced by annealing of bovine bone. Ceramics International 33, 1171–1177.

Panda NN, Pramanik K, Sukla LB. 2014. Extraction and characterization of biocompatible hydroxyapatite from fresh water fish scales for tissue engineering scaffold. Bioprocess Biosystem  Engineering, 37, 433–440.

Pandit N, Pandit IK, Malik R, Bali D, Jindal S. 2012.Autogenous bone block in the treatment of teeth with hopeless prognosis.Contemporary Clinical Dentistry 3(4), 437-442.

Piccirillo C, Silva M, Pullar R, da CruzIB, Jorge R, Pintado M, Castro PM. 2013. Extraction and characterisation of apatite-and tricalcium phosphate-based materials from cod fish bones. Material Science Engineering C, 33, 103–110.

Pilia M, Guda T, Appleford M. 2013. Development of composite scaffolds for load-bearing segmental bone defects. Biomed Research International, 2013, 1–15.

RahmanIA,Tijani HI, Mohammed BA, Saidu H, Yusuf H, Jibrin MN, Mohammed S. 2014. From garbage to biomaterials: an overview on egg shell based hydroxyapatite. Journal of Materials, 2014.

Ratner BD, Hoffman AS, Schoen FJ, Lemons JE. 2004. Biomaterials Science. An Introduction to Materials in Medicine. Second Edition, Academic Press, 851.

Raynaud S, Champion E, Assollant DB, Thomas P. 2002. Calcium phosphate apatite with variable Ca/P atomic ratio I. Synthesis, characterisation and thermal stability of powders. Biomaterials, 23, 1065–1072.

Reichert JC, Wullschleger ME, Cipitria A, Lienau J, Hutmacher DW. 2011. Custom-made composite scaffolds for segmental defect repair in long bones.International Orthopaedics, 35, 1229–1236.

Silva RV, Camilli JA, Bertran CA, Moreira NH. 2004. The use of hydroxyapatite and autogenouscancellous bone grafts to repair bone defects in rats. International Journal of Oral and Maxillofacial Surgery 10, 1–7.

Sofronia AM, Baies R, Anghel EM, Marinescu  CA, Tanasescu S. 2014. Thermal and structural characterization of synthetic and natural nanocrystalline hydroxyapatite”, Materials Science and Engineering: C, 43, 153–163.

Thrivikraman G, Madras G, Basu B. 2014.In vitro/ In vivo assessment and mechanisms of toxicity of bioceramic materials and its wear particulates.RSC Advances 4, article id. 12763.

Ugarte JFDO, Sena LÁD, Pérez CADC, Aguiar PFD, Rossi AM, Soares GA. 2005. Influence of processing parameters on structural characteristics of porous calcium phosphate samples: A study using an experimental design method. Material Research 8, 71–76.

Venkatesan J, Kim SK. 2010. Effect of temperature on isolation and characterization of hydroxyapatite from tuna (thunnusobesus) bone. Materials 3, 4761–4772.

Venkatesan J, Qian ZJ, Ryu B, Thomas NV, Kim SK. 2011. A comparative study of thermal calcination and an alkaline hydrolysis method in the isolation of hydroxyapatite from Thunnusobesus bone. Biomedical Materials 6, 2011.

Wua  SC, Hsua HC, Hsua SK, Changb YC, Ho WF. 2016. Synthesis of hydroxyapatite from eggshell powders through ball milling and heat treatment. Journal of Asian Ceramic Societies, 4(1), 85–90.

Xiaoying L, Yongbin F, Dachun G, Wei C. 2007. Preparation and Characterization of Natural Hydroxyapatite from Animal Hard Tissues.Key Engineering Materials, 342-343, 213-216.

Zainon I, Alwi N, Abidin M, Haniza H, Ahmad M, Ramli A. 2012. Physicochemical properties of hydroxyapatite extracted from fish scales. Advance Material Research 545, 235–239.

Submit Manuscript