J. Bio. Env. Sci.18(5), 8-18, May 2021
The application of natural biological dyes finds its use as an alternative to synthetic stains in temporary and permanent mounts. Extract of natural stains from peels of Lubeg (Syzygium lineatum), Bignay (Antidesma bunius), and Duhat (Syzygium cumini) fruits were made to test their staining potential on plant and animal tissues. The study utilized the Completely Randomized Design to compare the staining property of the peels. It used the Analysis of Variance (ANOVA) and Least Significant Differences (LSD) to ascertain the differences between the treatments. The natural biological dyes, in comparison with the synthetic dye, stained the cross-section of the Wiregrass and Narra stems, longitudinal section of an Autumn crocus, and hepatic tissues of a frog with remarkably deeper color. They are more effective in highlighting structures of a cross-section of a wiregrass stem and longitudinal section of an Autumn crocus, and they have a higher bulk density, which is an essential requirement of container volume. However, the synthetic dye shows higher absorbing capacity and solubility than the natural biological dyes. The success of producing a low-cost biological stain is achieved using Lubeg, Bignay, and Duhat fruits. The natural biological dyes used in this study could serve as a useful alternative to synthetic dyes.
Adeyemo SM, Akinloye AJ, Adekanmi GB. 2017. The use of plant dyes for microbial staining and identification: An eco-friendly and non-toxic alternative method. Journal of Advances in Biology & Biotechnology 16(4), 1-10. https://doi.org/10.9734 /JABB/2017/35014
Ajileye AB, Iteire AK, Arigi QB. 2015. Zingiber officinale (ginger) extract as a histological dye for muscle fibers and cytoplasm. International Journal of Medical Science and Public Health 4(10), 1445-1448. https://doi.org/10.5455/ijmsph.2015.21022015296
Akinloye AJ, Illoh HC, Olagoke AO. 2010. Screening of some indigenous herbal dyes for use in plant histological staining. Journal of Forestry Research 21(1), 81-84. https://doi.org/10.1007 /s11676 -010-0014-2
Amelia F, Afnani GN, Musfiroh A, Fikriyani AN, Ucche S, Murrukmihadi M. 2013. Extraction and stability test of anthocyanin from buni fruits (Antidesma bunius L) as an alternative natural and safe food colorants. Journal of Food and Pharmaceutical Sciences 1, 49-53. file:///C:/ Users/ User/Downloads/Documents/1844-3263-1-PB.pdf
Ashok PK, Upadhyaya K. 2012. Tannins are astringent. Journal of Pharmacognosy and Phytochemistry 1(3), 45-50.
Aqil F, Gupta A, Munagala R, Jeyabalan J, Kausar H, Sharma R, Singh IP, Gupta RC. 2012. Antioxidant and antiproliferative activities of anthocyanin/ellagitannin-enriched extracts from Syzygium cumini L. (‘jamun,’ the Indian Blackberry). Nutrition and Cancer 64(3), 428–438. https:// doi.org/10.1080/01635581.2012.657766
Ayyanar M, Subash-Babu P. 2012. Syzygium cumini (L.) skeels: A review of its phytochemical constituents and traditional uses. Asian Pacific Journal of Tropical Biomedicine 2(3), 240-246. https://doi.org/ 10.1016/S2221-1691(12)60050-1.
Barbosa‐Cánovas GV, Juliano P. 2005. Physical and chemical properties of food powders. In: Onwulata C, editor. Encapsulated and powdered foods. Taylor and Francis Group, LLC. P 39–71.
Barcelo JM, Abril JN, Castillo KMP, Diaz A, Ladera JP, Javar J, Labuguen E. 2014. Detection of copper (II) and iron (III) in aqueous solutions using the spectroscopic characteristics of Bugnay (Antidesma bunius) anthocyanins. Annals of Tropical Research 36(1),102-118.
Cowley L. 2019. What is a fabric dye? Eco World. https://ecoworldonline.com/what-is-fabric-dye/
Deepali K, Lalita S, Deepika M. 2014. Application of aqueous plant extracts as Biological stains. International Journal of Scientific & Engineering Research 5(2). http://www.ijser.org /paper/Application-of-aqueous-plant-extracts-as-Biological-stains.html.
Del Mundo L, Gorospe KJ, Locilla L, Serquina AK, Torres E. 2007. Basella Rubra Biological stain. http://www.investigatoryprojectexample.com/science/basella-rubra-biological-stain.html,
Englyst HN, Cummings JH. 1988. Improved method for measurement of dietary fiber as non-starch polysaccharides in plant foods. Journal Association of Official Analytical Chemists 71(4), 808-814. https://www.ncbi.nlm.nih.gov/pubmed
Hart A. 2015. Effect of particle size on detergent powders flowability and tabletability. Journal of Chemical Engineering & Process Technology 215. https://doi.org/10.4172/2157-7048.1000215
Kafle BP, Pokhrel BR, Gyawali R, Kafle A, Shrestha TM, Shrestha R, Adhikari RM. 2014. Absorbance of natural and synthetic dyes: Prospect of application as sensitizers in dye-sensitized solar cell. Advances in Applied Science Research 5(1), 8-12. http://www.imedpub.com/articles/absorbance-of-natural-and-synthetic-dyesprospect-of-application-as-sensitizers-in-dye-sensitized-solar-cell.pdf
Krizova H. 2015. Natural dyes: their past, present, future, and sustainability. https://www.researchgate. net/publication/293885858_Natural_dyes_their_past_present_future_and_sustainability.
Lyon HO. 2000. Standardization in biological staining: The influence of dye manufacturing. Biotechnic and Histochemistry 75(4), 176-82. https://www.researchgate.net/publication/12322911_Standardization_in_ Biological_Staining_ The_ Influence _ of_Dye_Manufacturing.
Manicad MCZ. 2016. Phytochemical analysis of Lubeg (Syzygium Lineatum (Dc).Merr & L.M. Perry) species in Apayao. International Journal of Novel Research in Life Sciences 3(6), 1-5. file:///C:/Users/User/Downloads/Documents/PHYTOCHEMICAL%20ANALYSIS-820_2.pdf
Mitra A, Das SK. 2015. Fabric dyeing with natural dye extracted from Basella alba fruit and spectroscopic analysis of the extract at different conditions. Journal of Chemical and Pharmaceutical Research 7(12), 1117-1124. http://www.jocpr.com /articles/fabric-dyeing-with-natural-dye-extracted-from-basella-alba-fruit-and-spectroscopic-analysis-of-the-extract-at-different.pdf
Ocampo RO, Usita NP. 2015. Development of Lubeg (Syzygium lineatum (Roxb.) Merr.& Perry) processed products. Asia Pacific Journal of Multidisciplinary Research 3(4).
Panche AN, Diwan AD, Chandra SR. 2016. Flavonoids: an overview. Journal of Nutritional Science 5, e47. https://doi.org/10.1017/jns.2016.41
Samanta AK, Agarwal P. 2009. Application of natural dyes on textiles. Indian Journal of Fibre and Textile Research 34(4), 384-399. https://doi.org/ 10.4314 /ajbr.v11i2.50706
Saravanan P, Chandramohan G, Saivaraj S, Deepa D. 2013. Extraction and application of eco-friendly natural dye obtained from barks of Odina wodier L. on cotton fabric. Journal of Natural Product and Plant Resources 3(2), 80-85. http://scholarsresearchlibrary.com/archive.html
Siva R. 2007. Status of natural dyes and dye-yielding plants in India. Current Science 92(7). https://www.researchgate.net/publication/228365046_Status_of_natural_dyes_and_dye-yielding_plants_in_India
Sharma A, Jana AH, Chavan RS. 2012. Functionality of milk powders and milk‐based powders for end-use applications—A review. Comprehensive Reviews in Food Science and Food Safety 11(5), 518-528. https://doi.org/10.1111/j.1541-4337.2012.00199.x
Shi H, Mohanty R, Chakravarty S, Cabiscol R, Morgeneyer M, Zetzener H, Ooi JY, Kwade A, Luding S, Magnanimo V. 2018. Effect of particle size and cohesion on powder yielding and flow. Kona 35, 226-250. https://doi.org/10.14356/kona.2018014
Suabjakyong P, Romratanapun S, Thitipramote N. 2011. Extraction of natural histological dye from Black Plum fruit (Syzygium cumini). Journal of the Microscopy Society of Thailand 4(1), 13-15. file:///C:/Users/User/ Downloads/Documents/JMST11BIO26_p13_15_suabjakyong_2.pdf
Sy MC, Wei AC. 2007. Basic Microbiology. Merriam & Webster Bookstore, Inc.
Wanlapa S, Wachirasiri K, Sithisam-ang D, Suwannatup T. 2015. Potential of selected tropical fruit peels as dietary fiber in functional foods. International Journal of Food Properties 18(6), 1306-1316. https://doi.org/10.1080/10942912. 2010.
Xu G, Lu P, Li M, Liang C, Xu P, Liu D, Chen X. 2018. Investigation on characterization of powder flowability using different testing methods. Experimental Thermal and Fluid Science 92: 390-401. https://doi.org/10.1016/j.expthermflusci. 2017.
Yusuf M, Shabbir M, Mohammad F. 2017. Natural colorants: Historical, processing, and sustainable prospects. Natural Products and Bioprospecting 7(1), 123-145. https://doi.org /10.1007 /s13659-017-0119-9