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Fertility enhancing effects of aqueous extract of leaves of Cnestis ferruginea Vahl ex De Cantolle on female wistar rats

Research Paper | December 1, 2016

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Zougrou N’guessan Ernest, Blahi Adélaïde Nadia, D’Almeida Marie-Anne, Kouakou Koffi

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Int. J. Biosci.9( 6), 79-91, December 2016

DOI: http://dx.doi.org/10.12692/ijb/9.6.79-91


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Cnestis ferruginea (Connaraceae) is one of the plants used as a therapeutic agent in many cultures in tropical Africa. To evaluate the pharmacological effects of the aqueous extract of C. ferruginea on reproductive parameters of female rats. Selected regular cycle female rats were randomized into 2 sets of 18 each and treated for 15 (set I) and 30 days (set II). Each set was then divided equally into three groups. Group 1 (control) was orally administered with distilled water once a day. Group 2 and 3 were respectively treated with 50 and 100 mg/kg body weight. Estrous cycle pattern was monitored before and during plant extract application whereas reproductive organs and reproductive hormones were determined at the end of each treatment. C. ferruginea induced a blockage of the estrous cycle at the estrous phase. Thus, animals treated showed highly significant increase (p<0.001) in the duration of estrous phase. AECF50 induced significant increase in the wet weight of ovary (42.690±4.21), p<0.05 and (44.470±922), p<0.001 and uterus (79.030±8.07), p<0.05 and (80.320±1.140), p<0.001 after 15 and 30 days of treatment respectively. Whereas, AECF100, induce only a significant increase (39.000±1.588, p<0.01) in the wet weight of ovary after 30 days treatment. For the dry weight, only AECF50 induced a significant increase (0.032±0.002, p<0.05) in the weight of uterus. For both duration of treatment and both treatment group, extract produced significant increase in serum concentration of FHS (p<0.001), LH (p<0.01), estradiol (p<0.01). Extract produced significant increase (p<0.01) in serum concentration of prolactin after 30 days treatment. The present study suggests that the extract of Cnestis ferruginea could contain estrogenic compounds favourable to fertility optimization.


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Fertility enhancing effects of aqueous extract of leaves of Cnestis ferruginea Vahl ex De Cantolle on female wistar rats

Akharaiyi FC, Boboye B, Adetuyi FC. 2012. Antibacterial, phytochemical and antioxidant properties of Cnestis ferruginea DC. (Connaraceae) extracts. Journal of Microbiology, Biotechnology and Food Sciences 2, 592-609.

Baker VA, Hepbern KSJ, Jones P, Lea L, Sumpter J, Ashby J. 1999. Safety evaluation of phytosterol esters. Part 1: Assessment of estrogenicity using a combination of in-vivo and in-vito assays. Food and chemical toxicology 17, 748-755.

Berhaut J. 1954. Flore du Sénégal, brousses et jardin (savanes de l’Afrique occidentale). Librairie Clairafrique, Dakar, 300.

Bleu GM. 2013. Etude phytochimique, toxicologique et pharmacologique de Passiflora foetida Linn. (Passifloraceae), une plante utilisée dans le traitement de l’infertilité féminine. Thèse de Doctorat, Université Félix Houphouët-Boigny Cocody-Abidjan 188.

Bleu GM, Kouakou K, Touré A, Traoré F. 2012. Effects of Passiflora foetida Lin. (Passifloraceae) on genital tract, serum estradiol, pituitary gonadotropin and prolactin level in female aldult and immature ovariectomized rats. Pakistan Journal of Scientific and Industrial Research Series B: Biological Sciences 55, 138-144.

Boakye-Yiadom, Konning GH. 1975. Incidence of antibactererial activity in the cannaraceae. Planta media Bull. 9 ref 28, 397-400.

Brann DW, Mills TM, Mahesh VB. 1995. Female reproduction: The ovulatory cycle. In: Witorsch RJ, Ed. Reproductive toxicology. New York: Raven press, 23-44.

Burkill HM. 1985. The Useful Plants of West Tropical Africa. Royal Botanic Gardens, kew(K). 1, 4.

Chearskul S, Kooptiwut S, Chatchawalvanit S, Onreabroi S, Churintrapun M, Saralamp P, Soonthornchareonnon N. 2004. Morinda citrifolia has very weak estrogenic activity in vivo. Thai journal of Physiological Sciences 17, 22-29.

Christenson LK, Stouffer RL. 1997. Follicle stimulating hormone and luteinizing hormone/chorionic gonadotropin stimulation of vascular endothelial growth factor prodaction by macque granulose cells from pre and peri ovulatory follicle. Journal of Clinical Endocrinology & Metabolism, 26, 1916-1922.

Declume C, Assamoi A, Akre TB. 1984. Anticonvulsant activity of Cnestis ferruginea, DC.(Connaraceae). Annals pharmaceutiques française 42, 35-41.

Diel P, Geis RB, Caldarelli A, Schimidt S, Leschowskey UL, Voss A, Vollmer G. 2004. The differential ability of the phytoestrogen genistein and of estradiol to induce uterine weight and proliferation in the rat is associated with a substance specific modulation of uterine gene expression. Molecular and cellular endocrinology, 22, 21-32.

Fitzgerald P, Dinan TG. 2008. Prolactin and dopamine: What is the connection? A review article. Journal of Psychopharmacology 22, 12-19.

Freeman ME. 2008. Neuroendrocrine control of the ovarian cycle of the rat. In: Knobil E, Jimmy D, Neil. (Ed.), 3nd edition “Physiology of reproduction”, Raven Press, New York, USA 2, 2328-2387.

Gayrard V. 2007. Physiologie de la reproduction des mammifères. Ecole nationale vétérinaire, Toulouse, France, 198.

Gill LS. 1992. Ethnomedical uses of plants in Nigeria, Benin, Nigeria: Uniben Press. 77.

Gupta S, westfall TC, Lechner AJ, Knuepfer MM. 2005. Teaching principle of cardiovascular function in a medical student laboratory. Advances in Physiology Education, 28, 118-127

Haim S, shakhar G, Rossene E, Taylor AN, Ben-Eliyah S. 2003. Serum level of sex hormones and corticosterone throughout 4 and 5 days estrous cycles in Fischer 344 rats and their stimulation in Ovariectomized females. Journal of endocrinology Investigation 26, 1013-1022.

Hodgen GD. 1989. Neuroendocrinology of the normal mestrual cycle. Journal of Reproductive Medicine 34, 68-75.

Hubscher CH, Brooks DL, Johnson JR. 2005. Aquantitive method for assessing stages of the rat estrous cycle. Biotechnic & Histochimistry, 80, 79-87.

Irvine FR. 1961. Woody plants of Ghana. Oxford University Press, Oxford. 146-147

Ishola IO, Ashorobi RB. 2007. The anti-stress potential of the aqueous root extract of Cnestis ferruginea. International Journal of Pharmacology. 3, 295-298.

Ishola IO, Akindele AJ, Adeyemi OO. 2011. Analgesic and anti inflammatory activities of Cnestis ferruginea Vahl ex DC (Connaraceae) methanolic root extract. Journal of Reproductive Medicine 135, 55-62.

Kanno J, Onyon L, Peddada S, Ashby J, Jacob E, Owens W. 2003b. The OECD program to validate the rat uterotrophic bioassay. Phase 2: dose-response studies. Environ Health Perspect 111, 1530-1549.

Kerharo J. 1974. La pharmacopée Sénégalaise traditionnelle, plantes médicinales et toxique. Vigot, Paris, 1012.

Kerharo J, Adam JG. 1974. La pharmacopée sénégalaise traditionnelle : plantes médicinales et toxiques Editions VIGOT frères, paris, 1011.

Kouakou K. 2000. Etude des effets antifertilisants de l’extrait de deux champignons (Daldinia concentrica, Bolt.1863 et Psathyrella efflorescens, Berk, 1977) de la pharmacopée ivoirienne chez la ratte. Thèse de Doctorat 3ème cycle, Université de Cocody-Abidjan, 122.

Lilaram, Nazeer AR. 2012. Effect of ethanolic seed extract of Caesalpinia bonducella on female reproductive system of albino rat: a focus on antifertility efficacy. Asian Pacific Journal of Tropical Disease 957-962.

Marcondes FK, Bianchi FJ, Tanno AP. 2002. Determination of the estrons cycle phases of rats : some helpful considerations. Brazilian Journal of Biology. 62, 609-614.

Monniaux D, Craty A, Clement F, Dalbiès-Tran R, Dupont J, Fabre S, Gereard N, Mermillod P, Monget P, Uzbekova S. 2009. Développement folliculaire et ovulation chez les mammifères. Inra Productions Animales 22, 59-76.

N’guessan K, Kouadio K, Kouamé NF. 2006. Plantes emménagogues utilisées en médecine traditionnelle par les peuples Abbey et Krobou d’Agboville (Côte d’ivoire) Pharmacopée et Médecine Traditionnelle Africaine. 14, 137-158.

Nazrullaev SS, Bessonova IA, Akhmedkhodzaeva KHS. 2001. Estrogenic activity as a function of chemical structure in haplophyllum quinoline alkaloids. Chemistry of Natural Compounds 37, 551-555.

Niswender GD, Juengel JL, Sila PJ, Rollyson MK, McIntush EW. 2000. Mechanisms controlling the function and life span of the corpus luteum. Physiologcal Review 80, 1-29.

Ogbechie AK, Olugbade TA, Oluwadiya JO. 1987. Chemical Constituents of Cnestis ferruginea DC II: Petroleumetterextract of the fruit Niger Journal of Pharmaceutical Sciences: 36-38.

Okafor J, Ham R. 1999. Identification, utilisation et conservation des plantes médicinales dans le sudest du Nigéria. Thème Biodiversité Africaine 3: 1-8.

Onyegeme-Okerenta BM, Essien EB. 2015. Effect of leaf extract of Millettia aboensis on the reproductive hormones and organs of female Wistar albino rats. International Journal of Biological Sciences and Applications 2, 42-47

Oyeyemi MO, Esan OO, Oyerinde CM, Uwalaka EC. 2015. Effects of Momordica  Charantia on the serum chemistryand some reproductive parameters in the female Wistar rats. Natural Sciences 13, 119-123.

Parverz M, Rahman A. 1992. A novel antimicrobial Isoflavone Galactoside from Cnestis ferruginea (Connaraceae); Journal of Chemical Society of Pakistan 14, 221-223.

Raji LO, Fayemi OE, Ameen SA, Jagun AT. 2012. The effects of aqueous extract of Allium sativum (Garlic) on some aspects of reproduction in the female albino rat (Wistar strain). Global veterinaria 8, 414-420.

Russell WF. 2008. The female reproductive cycle: a pratical histological Guide to standing. Journal of Toxicologic Pthology, 36: 375-384.

Sahar MMO, Abeer AAS. 2007. Modified vaginal smear cytology for the determination of the estrus cycle phases, versus ordinary Papanicolaou technique, verified by light and scanning electron microscopic examination of the endometrium. Egyptian Journal of Histology, 30, 397-408.

Sandhu DS, Heinrich M. 2005. The use of health foods, spices and other botamicals in the Sikh community in London. Phytotherapy Res., 19, 633-642.

Shupnik MA, Rosenzweig BA. 1991. Identification of an estrogen-response element in the rat LHB gene. Journal of Cemistry 266, 17084-17091.

Wise PM, Camp-grossman P, Barraclough C. 1981. Effects of estradiol and progesterone on plasma gonadotropins, prolactin and LHRH in specific brain areas of ovariectomized rats. Biology of Repeoduction 24, 820-830.

Yakubu MT, Nurudeen QO. 2012. Effects of aqueous extract of Cnestis ferruginea (Vahl ex De Cantolle) root on paroxetine-induced sexual dysfunction in male rats. Asian Pacific Journal of Reproduction 1, 111-116.

Yakubu MT, Adams DM, Akanji MA, Oladiji AT. 2011. Laxative activity of the aqueous root extract of Cnestisferruginea (Vahl ex DC) inloperamide-induced constipated rats. Nigerian Journal of Gastroenterology and Hepatology 3(1-2), 21-29.

Yakubu MT, Akanji MA, Oladiji AT, Olatinwo AWO., Adesokan AA, Yakubu MO, Owoyele BV, Sunmosu TO, Ajao MS. 2008. Effect of Cnidoscolous aconitifolus (Miller) I.M. Johnston leaf extract on reproductive hormones of female rats. Iran Journal of Reproductive Medecine 6, 149-155.

Young J, Gougeon A, Schaison G. 1999. Le cycle ovarien. In : Médecine Sciences L’ovaire MASSON Editions 15, 139-302.