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First report of Carbapenemase producing gram negative bacteria in Republic of Benin: Pseudomonas aeruginosa Vim-2 New ST

Research Paper | October 1, 2018

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H. Koudokpon, V. Dougnon, E. Azehoun Pazou, I. Kissira, L. Hadjadj, A. Fanou, F. Loko, H. Bankole, S. Diene, JM. Rolain

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Int. J. Biosci.13( 4), 437-443, October 2018

DOI: http://dx.doi.org/10.12692/ijb/13.4.437-443


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The spread of carbapenem resistance is increasingly a concern and a serious health challenge for Africa. The present study aims to detect resistance to carbapenem in Benin. 157 Gram negative bacilli were isolated in the period of July to September 2015 in southern Benin. After identification with Maldi Tof Mass Spectrometer, sensitivity to carbapenems (Ertapenem and Imipenem) was determined by antibiotic disk diffusion on MH-2 Agar. Resistance to Imipenem were confirmed by E-test and Carba NP test. The presence of OXA-48 was investigated in all strains exhibiting resistance to one of the carbapenems tested and the search for KPC, NDM, IMP and VIM in strains resistant to Imipenem by real-time and gel-based PCR. The OPRD gene was also investigated by PCR in Pseudomonas aeruginosa strains. The genes found by PCR were sequenced and MLST was performed.17 of the 157 strains had a phenotype of Ertapenem resistance against only 5 strains resistant to Imipenem. Resistance to carbapenems was observed in 8 Escherichia coli isolates, 5 Enterobacter cloacea, 2 Enterobacter asburiae and one Klebsiella pneumoniae isolate. 3 strains of Pseudomonas aeruginosa were isolated and showed resistance to imipenem and Ertapenem. The search for resistance genes showed that only Pseudomonas strains carried the VIM-2 gene. MLST analysis of the Pseudomonas sequences showed an unidentified clone. This demonstrates the need for urgent surveillance and control of resistance to antibiotics in Benin.


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First report of Carbapenemase producing gram negative bacteria in Republic of Benin: Pseudomonas aeruginosa Vim-2 New ST

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