First report of lasiodiplodia theobromae causing shoot blight of ricinodendron heudelotii seedlings in Cameroon
Paper Details
First report of lasiodiplodia theobromae causing shoot blight of ricinodendron heudelotii seedlings in Cameroon
Abstract
The aim of this study was to identify the pathogen responsible to shoot blight, a new disease observed on Ricinodendron heudelotii seedlings in Cameroon. Isolation of the fungus from infected tissues was made on potato dextrose agar (PDA) milieu. After identification based on morphological characteristics (mycelium structure and conidia sizes), DNA was extracted and submitted to molecular analysis. The sequences obtained from rDNA were identified as Lasiodiplodia theobromae. Inoculums were then prepared and Koch´s postulate was verified both on detached leaves and seedlings by inoculating healthy detached leaves and seedlings with 5 mm diameter mycelia plugs and a solution of 106 conidial/ml respectively. Shoot blight symptoms were observed both on detached leaves and on seedlings. This was the first report of L. theobromae causing shoot blight on R. heudelotii seedlings.
Agrios GN. 2005. Plant pathology. 5th Ed. Elsevier Academic Press, California, USA, 922p.
Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ. 1997. Gapped BLAST and PSI-BLAST: a new generation of protein data base search programs. Nucleic Acids Ressources 25, 3389-3402.
Alexopoulos CJ, Mims CW, Blackwell M. 1996. Introductory Mycology. 4th Ed. Wiley, New York.
Burruano S, Mondello V, Conigliaro G, Alfonzo A, Spagnolo A, Mugnai L. 2008. Grapevine decline in Italy caused by Lasiodiplodia theobromae. Phytopathologica Mediterranea 47, 132-136.
Djeugap FJ, Bernier L, Dostaler D, Khasa D, Fontem DA, Nwaga D. 2013. Opportunités et contraintes agroforestières de Ricinodendron heudelotii au Cameroun. International Journal of Biological and Chemical Science 7(1), 344-355.
Djeugap FJ, Bernier L, Dostaler D, Fontem DA, Avana ML. 2014. Germination Constraints of Ricinodendron heudelotii in Cameroon. Seed Technology 36 (1), 25-36.
Djeugap FJ, Tsopmbeng NG, Keuete KE, Yaouba A, Serferbe S. 2015. Isolation and Identification of Fungi Associated with Avocado Fruits from Local Markets of the West Region of Cameroon. International Journal of Agricultural and Biosciences 4 (2), 64-68.
Gezahgne A, Yirgu A, Kassa H. 2014. First report of Lasiodiplodia theobromae causing canker on tapped Boswellia papyrifera trees in Ethiopia. New Disease Reports 29, 11.
Khanzada MA, Lodhi AM, Shahzad S. 2004. Mango dieback and gummosis in Sindh Pakistan caused by Lasiodiplodia theobromae. Plant Health Progress [http://www.plantmanagementnetwork.org /pub/php/diagnostic guide/2004/mango/].
Latha P, Prakasam V, Kamalakannan A, Gopalakrishnan C, Raguchander M, Paramathma M, Samiyappan R. 2009. First report of Lasiodiplodia theobromae (Pat.) Griffon & Maubl. causing root rot and collar rot disease of physic nut (Jatropha curcas L.) in India. Australasian Plant Disease Notes 4, 19-20.
Levy L, Mavrodieva V. 2004. PCR detection and DNA isolation methods for use in the Phytophthora ramorum national program. DNA and PCR Protocol elaborated at Oregon State University and the University of California at Berkeley for P. ramorum, 11p.
Mbenoun M, Zeutsa EHM, Samuels G, Amougou FN, Nyasse S. 2007. Dieback due to Lasiodiplodia theobromae, a new constraint to cocoa production in Cameroon. New Disease Reports 15, 59.
Prober JM, Trainor GL, Dam RJ, Hobbs FW, Robertson CW, Zagursky RJ, Cocuzza AJ, Jensen MA, Baumeister K. 1987. A system for rapid DNA sequencing with fluorescent chain terminating dideoxynucleotides. Science 238, 336-341.
Tchatat M, Ndoye O. 2006. Étude des produits forestiers non ligneux d’Afrique centrale: réalités et perspectives. Bois et forêts des tropiques 288, 27-39.
Tchiegang C, Kapseu C, Ndjouenkeu R, Ngassoum MB. 1997. Les amandes de Ricinodendron heudelotii (Baill.): matière première potentielle pour les huileries tropicales. Journal of Food Engineering 32, 1-10.
White TJ, Bruns TD, Lee S, Taylor J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. Pages 315-322 in: PCR Protocols: A Guide to Methods and Applications. Innis MA, Gelfand DH, Sninsky JJ and White TJ eds. Academic Press, San Diego, CA.
Joseph Fovo Djeugap, Louis Bernier, Daniel Dostaler, Gabin Régis Dongmo Zena, 2016. First report of lasiodiplodia theobromae causing shoot blight of ricinodendron heudelotii seedlings in Cameroon. Int. J. Agron. Agric. Res., 8(1), 59-63.
Copyright © 2016 by the Authors. This article is an open access article and distributed under the terms and conditions of the Creative Commons Attribution 4.0 (CC BY 4.0) license.