Fluorescence based method for specific sequence detection of genetically modified tomato

Fluorescence based method for specific sequence detection of genetically modified tomato

Mehdi Dadmehr, Behnaz Korouzhdehi, Hamidreza Ganjali

DOI: https://dx.doi.org/10.12692/ijb/5.6.156-161

Int. J. Biosci. 5(6), 156-161. September, 2014. (PDF)

Abstract:

In recent years genetic engineering resulted to the production of genetically modified organism (GMO) included many important crops. Several attempts have been focused on development of methods for detection of genetically modified organisms (GMOs). In this study fluorescence based method for specific sequence recognition of plant CaMV promoter in transgenic tomato was developed which was upon hybridization of specific probe and amplified targets from transgenic tomato plants. Methylene Blue (MB) as a fluorescence marker has been used and showed quenching effect after addition of different concentration of target ssDNA to the solution. After optimizing of environmental conditions the results showed that decreased fluorescence was proportional with the concentration of 3 × 10−9 to 1× 10−7M of target ssDNA with the detection limited of 2 × 10-9 M.