Formulation and in-vivo evaluation of theophylline transdermal patch

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Research Paper 25/09/2022
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Formulation and in-vivo evaluation of theophylline transdermal patch

JA. Avbunudiogba, E. Agbamu, BB. Iwetan, T. Obavwonovwe, AO. Ifie
Int. J. Biosci.21( 3), 215-222, September 2022.
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This was an analytical study to evaluate the transdermal patch of theophylline. Theophylline transdermal patch was prepared by solvent casting method using hydroxyl propyl methylcellulose (HPMC) as polymer in a ratio of 1:3 (drug: polymer), propylene glycol as a permeation enhancer and 0.05M sodium hydroxide solution as solvent. Physicochemical analyses such as color, clarity, flexibility, smoothness, thickness, folding endurance, weight uniformity, percentage moisture uptake, percentage moisture loss, flatness and determination of drug content were performed. The in-vivo study was performed using six Wistar rats, 8-10 weeks old weighing 100-180 g, maintained at a temperature of 30.4 ± 1oC and relative humidity of 84% for the study. The patch was removed from the animals and analyzed for drug content using High Performance Liquid Chromatography (HPLC) at 30 min, 1 h and 2 h. The urine of the animals was collected at the same time interval to assay drug content. Mean weight uniformity (mm) was 0.0433 ± 0.0013, mean thickness uniformity (mm) was 0.34 ± 0.018, folding endurance was 198.67 ± 3.09,% moisture loss and uptake was 12.46 ± 2.98 and 2.12 ± 1.92 respectively. Drug content (%) was 105 ± 0.37. Drug concentration in patch after administration ranged from 89.99% to 97.44% for males and 89.74% to 97.14% for females. Urine drug concentration was highest after 2 h (10.71%) for males and 4.08% for females. The results indicate the feasibility of formulating a theophylline transdermal delivery system.


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