Fortification of cookies with sweet basil leaves powder: an unheeded hematinic

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Research Paper 01/04/2020
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Fortification of cookies with sweet basil leaves powder: an unheeded hematinic

Farah Naz Akbar, Shahid Mahmood, Ghulam Mueen-ud-din, Muhammad Yamin, Muhammad Nadeem, Huma Bader Ul Ain, Tabussam Tufail
Int. J. Biosci.16( 4), 366-379, April 2020.
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Iron deficiency prevails worldwide which may be reduced through dietary interventions like food fortification. Sweet basil (Ocimum basilicum L.) is an iron rich medicinal plant. The present study explores its potential for fortification of cookies as Fe is used to enhance the bioactive components. There were five treatments prepared in this study, which were presented in sweet basil leaves powder i.e. T0= Control, T1= 12.699 FeSO4.7H2O +٭EDTA mg, T2= 14 g, T3= 16 g, and T4=18 g. Cookies of all treatments were examined for proximate composition and sensory aspects along with the measurement of calorific and TBA values. The storage stability of cookies was also determined for 60 days. Iron content and other minerals were determined by atomic absorption spectrophotometry in fresh and dried leaves. The findings showed that T3 was the most appropriate treatment of sweet basil leaves powder cookie. The score of color, taste, and texture were 6.07±0.06, 5.93±0.08, and 6.07±0.04, respectively for T3. A physico-chemical test showed that cookies of T3 contained water (4.74±0.08%), protein (13.79±0.02%), carbohydrate (52.31±0.099%), crude fibre (6.08±0.003%), fat (20.68±0.04%), and ash (2.40±0.02 %). Study concluded that ash, protein, fiber and iron contents are increased with increased the amount of sweet basil leaves powder. Calorific value and NEF were declined by adding the sweet basil leaves powder. Therefore, it has potential of being healthy caloric snack which can accomplish the nutritional requirements of all age groups and helps in the alleviation of anemia.


AACC. 2000. Approved methods of the AACC: AACC St. Paul.

Amrani S, Harnafi H, Bouanani NEH, Aziz M, Caid HS, Manfredini S, Besco E, Napolitano M, Bravo E. 2006. Hypolipidaemic activity of aqueous ocimum basilicum extract in acute hyperlipidaemia induced by triton WR‐1339 in rats and its antioxidant property. Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives 20, 1040-1045.

Butt M, Sharif K, Huma N, Mukhtar T, Rasool J. 2004. Storage studies of red palm oil fortified cookies. Nutrition & Food Science 34, 272-276.

Butt MS, Arshad MU, Alam MS, Nadeem MT. 2007. Bioavailability and storage stability of vitamin A fortificant (retinyl acetate) in fortified cookies. Food Research International 40, 1212-1219.

Chelladurai C, Pandey AA, Panmand SA, Nikam S. 2019. Development of innovative bakery product chia seed enriched cookies. Development 4.

El Khouly NI. 2017. Comparison of intravenous ferrous sucrose and oral ferrous sulphate in treatment of postpartum iron deficiency anemia. The Journal of Maternal-Fetal & Neonatal Medicine 30, 967-971.

Faqih AM, Kakish S, Izzat M. 2006. Effectiveness of intermittent iron treatment of two-to six-year-old Jordanian children with iron-deficiency anemia. Food and nutrition bulletin 27, 220-227.

Feldsine P, Abeyta C, Andrews WH. 2002. AOAC International methods committee guidelines for validation of qualitative and quantitative food microbiological official methods of analysis. Journal of AOAC International 85, 1187-1200.

Fellers D, Bean M. 1977. Storage stability of wheat based foods: A review. Journal of Food Science 42, 1143-1147.

Güez CM, Souza ROd, Fischer P, Leão MFdM, Duarte JA, Boligon AA, Athayde ML, Zuravski L, Oliveira LFSd, Machado MM. 2017. Evaluation of basil extract (Ocimum basilicum L.) on oxidative, anti-genotoxic and anti-inflammatory effects in human leukocytes cell cultures exposed to challenging agents. Brazilian Journal of Pharmaceutical Sciences 53.

Hightower NC, Dworken HJ, Sircus W, Keeton WT. 2020. Human digestive system Encyclopædia Britannica, inc.

Javanmardi J, Khalighi A, Kashi A, Bais H, Vivanco J. 2002. Chemical characterization of basil (Ocimum basilicum L.) found in local accessions and used in traditional medicines in Iran. Journal of agricultural and food chemistry 50, 5878-5883.

Karimian-Khosroshahi N, Hosseini H, Rezaei M, Khaksar R, Mahmoudzadeh M. 2016. Effect of different cooking methods on minerals, vitamins, and nutritional quality indices of rainbow trout (Oncorhynchus mykiss). International Journal of Food Properties 19, 2471-2480.

Keast RS, Breslin PA, Beauchamp GK. 2001. Suppression of bitterness using sodium salts. Chimia International Journal for Chemistry 55, 441-447.

Kent NL. 1994. Kent’s Technology of Cereals: An introduction for students of food science and agriculture. Elsevier.

Kirk R. 1999. Sawyer R Pearsons composition and analysis of foods: Addison-Wesley Lingmam Inc., Harlow, England.

Krishna G, Ranjhan S. 1981. Gross energy value of herbage, faeces, urine, milk, meat and silage. Laboratory Manual for Nutrition Research.

Kulkarni S. 1997. Roasted soybean in cookies: Influence on product quality. Journal of food science and technology 34, 503-505.

Lee J, Scagel CF. 2009. Chicoric acid found in basil (Ocimum basilicum L.) leaves. Food Chemistry 115, 650-656.

Mahmood S. 2009. Efficacy studies of vitamin A fortified cookies in pregnant and lactating women. University of Agriculture Faisalabad.

Mahmood S, Butt MS, Anjum FM, Nawaz H. 2008. Baking and storage stability of retinyl acetate (vitamin A) fortified cookies. Pakistan Journal of Nutrition 7, 586-589.

Manley D. 2011. Manley’s technology of biscuits, crackers and cookies. Elsevier.

Meilgaard M, Civille G, Carr B. 2007. The spectrum descriptive analysis method. Sensory evaluation techniques 4, 189-253.

Mohamed S, El Tawila M, Ismail H, Gomaa N. 2010. Evaluation of the level of micronutrients in fortified foods in Alexandria, Egypt.

Paradiso VM, Summo C, Trani A, Caponio F. 2008. An effort to improve the shelf life of breakfast cereals using natural mixed tocopherols. Journal of Cereal Science 47, 322-330.

Pareyt B, Delcour JA. 2008. The role of wheat flour constituents, sugar, and fat in low moisture cereal based products: a review on sugar-snap cookies. Critical reviews in food science and nutrition 48, 824-839.

Pasha I, Butt M, Anjum F, Shehzadi N. 2002. Effect of dietetic sweeteners on the quality of cookies. International Jarnal of Agricculture Biololgy 4, 245-248.

Patel M, Gadhvi H, Patel S, Mankad A, Pandya H, Rawal R. 2018. Holy basil: Holy herb to multimodal medicine for human health.

Patimah S, Arundhana AI, Mursaha A, Syam A. 2019. Development of Foxtail Millet and Flying Fish Flour-Based Cookies as Functional Food. Current Research in Nutrition and Food Science Journal 7.

PF Guine R, J Goncalves F. 2015. Chemistry and health effects of bioactive compounds in selected culinary aromatic herbs. Current Nutrition & Food Science 11, 145-164.

Sahé AC, Agbenorhevi JK, Wireko-Manu FD. 2019. Quality Characteristics of Cassava-Ackee-Groundnut Composite Cookies. American Journal of Food Science and Technology 7, 57-64.

Shan B, Cai YZ, Sun M, Corke H. 2005. Antioxidant capacity of 26 spice extracts and characterization of their phenolic constituents. Journal of agricultural and food chemistry 53, 7749-7759.

Sharif K, Sadiq Butt M, Huma N. 2005. Oil extraction from rice industrial waste and its effect on physico-chemical characteristics of cookies. Nutrition & Food Science 35, 416-427.

Šimurina OD, Filipčev BV, Lević LB, Pribiš VD. 2008. Application of sugar beet molasses in the production of tea biscuits. Food and Feed Research 35, 201-205-201-205.

Sonmezdag AS, Amanpour A, Kelebek H, Selli S. 2018. The most aroma-active compounds in shade-dried aerial parts of basil obtained from Iran and Turkey. Industrial crops and products 124, 692-698.

Vijerathna M, Wijesekara I, Perera R, Maralanda S, Jayasinghe M, Wickramasinghe I. 2019. Physico-chemical Characterization of Cookies Supplemented with Sugarcane Bagasse Fibres. Vidyodaya Journal of Science 22.

Ziena HM, Shamsia SM, Mahgoub SA, Emara MA. 2019. Nutritious Biscuits for Celiac Patients: Effect of Different Cereals and Legumes Blends. Alexandria Science Exchange Journal 40, 340-346.

Zoulias EI, Oreopoulou V, Kounalaki E. 2002. Effect of fat and sugar replacement on cookie properties. Journal of the Science of Food and Agriculture 82, 1637-1644.

Zoulias EI, Piknis S, Oreopoulou V. 2000. Effect of sugar replacement by polyols and acesulfame‐K on properties of low‐fat cookies. Journal of the Science of Food and Agriculture 80, 2049-2056.