home icon user icon
Welcome to International Network for Natural Sciences | INNS Pub.
Home My Account
Submit Manuscript
double_slash
breadcumb_bg

Fungi associated with spoiled fruits vended in local market

Paper Details

Research Paper 01/01/2020
Views (410) Download (39)
current_issue_feature_image
publication_file

Fungi associated with spoiled fruits vended in local market

Idrees Ahmed Qureshi, Sham Lal, Sapna, Pardeep Kumar, Javed Ahmed Ujjan, Om Parkash, Nisar Ahmed Kanhar
Int. J. Biosci.16( 1), 244-250, January 2020.
Certificate: IJB 2020 [Generate Certificate]
Key: Fungi; Fruit; Spoilage; Prevalence; Pathogenic

Abstract

Fruits spoilage causes threat to human health and responsible for great economic loss worldwide. This study was conducted to isolate and identify fungi responsible for spoilage of fruit commonly sold in Panj Gula market, Khairpur, Sindh, Pakistan. A total of 60 spoiled fruits, 10 samples from each different fruit includingstrawberry (Fragariaananassa), orange (Citrus sinensis), papaya (Carica papaya), melon (Cucumis melo), apple (Malus domestica) and guava (Psidium guajava) were collected aseptically in sterile polyethene bag and delivered to microbiology laboratory at refrigerated temperature. Fungi were isolated and identifiedby using culturing and microscopic methods. Seventy seven fungal isolates belonging to five different genera, Aspergillus spp., Penicillium spp., Alternaria spp., Rhizopus spp., and Yeastwere isolated from spoiled fruits. Aspergillus spp. had the highest occurrence in all the fruits tested with a frequency of 40.26% followed by Penicillium spp. (24.67%), Alternaria spp. (20.78%), Rhizopus spp. (9.1%), and Yeast (5.19%). The results of this study suggest that fruits are spoiled with variety of fungi which causes great economic loss of country. In addition, isolation of pathogenic fungi in this study highlights the risk to human and animal health associated with spoiled fruits. Therefore improved preservation methods are required to prolong the shelf life of fruits and minimize the health hazard.

VIEWS 60
Cover PDF

Adebayo-Tayo BC, Odu NN, Esen CU, Okonko IO. 2012. Microorganisms associated with spoilage of stored vegetables in Uyo Metropolis, Akwa Ibom State, Nigeria. Nature and Science 10(3), 23-32. http://www.sciencepub.net/nature

Akinmusire OO. 2011. Fungal species associated with the spoilage of some edible fruits in Maiduguri Northern Eastern Nigeria. Advances in Environmental Biology 1, 157-62.

Amadi JE, Oso BA. 1996. Mycoflora of Cowpea Seeds (Vigna unguiculata L.) and their effects on seed nutrient content and germination. Nigerian Journal of Science 30, 63-9.

Anwer SS, Ali GA, Hamadamin CZ, Jaafar HY. 2017. Isolation and identification of fungi from fast food restaurants in Langa Bazar. International Journal of Environment, Agriculture and Biotechnology 2(4). http://dx.doi.org/10.22161/ijeab/2.4.10

Akhter MS, Alam S, Islam MS, Lee MW. 2009. Identification of the fungal pathogen that causes strawberry anthracnose in Bangladesh and evaluation of in vitro fungicide activity. Microbiology 37(2), 77-81. https://doi.org/10.4489/MYCO.2009.37.2.077

Ayoola NA. 2007. Survey of the post harvest diseases and aflatoxin contamination of marketed pawpaw fruit (Carica papaya L) in South Western Nigeria. African Journal of Agricultural Research 2(4), 178-81. http://www.academicjournals.org/AJAR

Beuchat LR, Pitt JI. 2001. Detection and enumeration of heat-resistant molds. Compendium of Methods for the Microbiological Examination of Foods 3, 251-63.

Bıyık HH, Un Z, Torun B, Poyrazoglu E. 2018. Fungal Biodiversity of Strawberry Fields in Aydin, TURKEY. International Journal of Secondary Metabolite 5(2), 60-6. http://dx.doi.org/10.21448/ijsm.398367

Amadi JE, Nwaokike P, Olahan GS, Garuba T. 2013.  Isolation and identification of fungi involved in the post-harvest spoilage of guava (Psidium guajava) in Awka metropolis. International Journal of Engineering 4(10), 8269.

Bukar A, Mukhtar MD, Adamu S. 2009. Isolation and identification of postharvest spoilage fungi associated with sweet oranges (Citrus sinensis) traded in Kano metropolis. Bayero Journal of Pure and Applied Sciences 2(1), 122-4.

Chukwuka KS, Okonko IO, Adekunle AA. 2010. Microbial ecology of organisms causing pawpaw (Carica papaya L.) fruit decay in Oyo State, Nigeria. American-Eurasian Journal of Toxicological Sciences 2(1), 43-50.

Droby S. 2005. Improving quality and safety of fresh fruits and vegetables after harvest by the use of biocontrol agents and natural materials. International Symposium on Natural Preservatives in Food Systems 45-51. http://dx.doi.org/10.17660/ActaHortic.2006.709.5

El-Gali ZI. 2016. Isolation and Identification of Fungi Associated with Fruits Sold in Local Markets. International Journal of Research Studies in Biosciences 4(11), 61-64. http://dx.doi.org/10.20431/2455-4316.0402001

Frisvad JC, Samson RA. 1991. Mycotoxins produced by species of Penicillium and Aspergillus occurring in cereals.. In: Chelkowski., J.(ed.) Cereal Grain. Mycotoxins, Fungi and Quality in Drying and Storage. Elsevier, Amsterdam fungi. Burgess Publishing Company. USA 441-476.

Ghosh A. 2009. Identification of microorganisms responsible for spoilage of tomato (Lycopersicon esculentum) fruit. Journal of Phytology.

Kalyoncu F. 2005. Determination of Fungi Associated with Tomatoes (Lycopersicum esculentum M.) and Tomato Pastes Fatih Kalyoncu, A. Usame Tamer and Mustafa Oskay. Plant Pathology Journal 4(2), 146-9.

Ibrahim AD, Musa K, Sani AA, Aliero AA, Yusuf BS. 2011. Microorganisms associated with the production of volatile compounds in spoilt tomatoes. Research in Biotechnology 2(2).

Mbajiuka S, Enya E. 2014. Isolation of microorganisms associated with deterioration of tomato (Lycopersicon esculentum) and pawpaw (Carica papaya) fruits. International Journal of Current Microbiology and Applied Sciences 3(5), 501-512. http://www.ijomas.com/vol-3-5/S.Mbajiuka,etalpdf.\

Samuel O, Ifeanyi O, Ugochukwu O. 2015. Filamentous Fungi Associated with the Spoilage of Post-Harvest Sweet Orange Fruits (Citrus Sinensis) Sold in Awka Major Markets, Nigeria. Bioengineering and Bioscience 3(3), 44-49. http://dx.doi.org/10.13189/bb.2015.030303

Tafinta IY, Shehu K, Abdulganiyyu H, Rabe AM, Usman A. 2013. Isolation and identification of fungi associated with the spoilage of sweet orange (Citrus sinensis) fruits in Sokoto State. Nigerian Journal of Basic and Applied Sciences 21(3), 193-6. http://dx.doi.org/10.4314/njbas.v21i3.4

Oyeleke A, Manga SB. 2008. Essential of Laboratory Practice. 3rd ed. Tobest Publisher, Minna, Niger state, Nigeria 12-29.

Mailafia S, God’spower Richard Okoh HO, Olabode K, Osanupin R. 2017. Isolation and identification of fungi associated with spoilt fruits vended in Gwagwalada market, Abuja, Nigeria. Veterinary World 10(4), 393.

Pitt JI, Hocking AD. 1997. Fungi and food spoilage. London, UK: Blackie Academic and Professional 2.

Samson RA, Varga J. 2007.  Editors. Aspergillus systematics in the genomic era. Utrecht: CBS Fungal Biodiversity Centre.

Sharma G, Kumar N, Weir BS, Hyde KD, Shenoy BD. 2013. The ApMat marker can resolve Colletotrichum species: a case study with Mangifera indica. Fungal Diversity 61(1), 117-38.

Singh D, Sharma RR. 2007. Postharvest diseases of fruit and vegetables and their management. In: Prasad, D. (Ed.), Sustainable Pest Management. Daya Publishing House, New Delhi, India.

Spotts RA, Cervantes LA. 1986. Population pathogenicity, andbenomyl resistance ofBotrytisspp., Penicilliumspp. And Mucorpiriformisin packing houses. Plant Diseases 70, 106–108.

Tournas VH, Stack ME. 2001. Production of alternariol and alternariol methyl ether by Alternaria alternata grown on fruits at various temperatures. Journal of Food Protection 64(4), 528-32. https://doi.org/10.4315/0362-028X-64.4.52.8

Tournas VH, Katsoudas E. 2005. Mould and yeast flora in fresh berries, grapes and citrus fruits. International Journal of Food Microbiology 105(1), 11-7.

Wogu MD, Ofuase O. 2014. Microorganisms responsible for the spoilage of tomato fruits, Lycopersicum esculentum, sold in markets in Benin City, Southern Nigeria. Scholars Academic Journal of Biosciences 2(7), 459-66. http://Saspublisher.com/sajb-27.

Zhu SJ. 2006. Non-chemical approaches to decay control in postharvest fruit. Advances in postharvest technologies for horticultural crops. Research Signpost, Trivandrum, India 297-313.

Submit Manuscript