Fungistatic potential of Sonchus asper against Botrytis cinerea and Rhizoctonia solani

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Research Paper 01/01/2017
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Fungistatic potential of Sonchus asper against Botrytis cinerea and Rhizoctonia solani

Tayyaba Ejaz, Khajista Jabeen, Sumera Iqbal, Aroosa Naeem
Int. J. Biosci.10( 1), 327-334, January 2017.
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Abstract

Sonchus asper L. was evaluated for its antifungal potential against Botrytis cinerea Pers. and Rhizoctonia solani Kuhn. Various concentrations viz. 1%, 2%, 3%, 4%, 5% of methanolic extract of aerial part of S. asper were tested against B. cinerea and R. solani. All the applied concentrations of tested extracts significantly inhibited the growth of tested fungi up to 37%-74%. Methanolic extract of aerial parts was partitioned with n-hexane, ethyl acetate, chloroform and n-butanol. These isolated organic fractions were further serially diluted from 100 mg to 0.78 mg mL-1 to evaluate their Minimum Inhibitory Concentration (MIC). A synthetic fungicide (Puslan, 72 WP) was used as reference and data was recorded at interval of 24, 48 and 72 hours. Ethyl acetate and Puslan (Synthetic fungicides) were found highly effective against both the test fungi with MIC of 0.78 mg after 72 hrs of incubation period. Chloroform and n-hexane fractions also inhibited the fungal growth.

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