GC-MS Analysis and Evaluation of In-vitro Antioxidant Potential and Total Phenolics Content of Wild Hops (Flemingia strobilifera (L.) W. T. Aiton)

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Research Paper 01/01/2016
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GC-MS Analysis and Evaluation of In-vitro Antioxidant Potential and Total Phenolics Content of Wild Hops (Flemingia strobilifera (L.) W. T. Aiton)

Jhoan Rhea L. Pizon, Olga M. Nuñeza, Mylene M. Uy, W.T.P.S.K Senarath
Int. J. Biosci.8( 1), 25-32, January 2017.
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Wild hops (Flemingia strobilifera Linn.) is a shrub belonging to Fabaceae family. The leaves of F. strobilifera are commonly used by the Subanen, the indigenous group in Lapuyan, Zamboanga del Sur, Philippines to treat inflammation. In this study, the hydromethanolic (80%) and aqueous leaf extracts of F. strobilifera were evaluated for their antioxidant activity and total phenolics content. The active semi-volatile components of 80% methanol leaf extract were analyzed using Gas Chromatography-Mass Spectrometry (GC-MS). DPPH radical scavenging activity was used to determine the potential of this plant as anti-oxidant. Total phenolics content was determined using Folin-Ciocalteu reagent and calculated as gallic acid equivalence. GC-MS analysis revealed the presence of eight compounds. Both the 80% methanol and aqueous extracts of F. strobilifera showed significant scavenging activity with low IC50 values of 0.299 mg/mL and <0.25 mg/mL, respectively. There was positive correlation between the scavenging activity percentage and the total phenolics content only in the aqueous extract of F. strobilifera while 80% methanol extract showed negative correlation between inhibition percentage and total phenolics content which can be attributed to the solvent used and method used in quantification of phenolics. Nevertheless, the results suggest that these leaf extracts are potent source of antioxidant compounds and may serve as natural anti-inflammatory agents.


Amarowicz R, Pegg BR, Rahimi-Moghaddam P, Bar B, Weil JA. 2003. Free-radical scavenging capacity and antioxidant activity of selected plant species from the Canadian prairies. Food Chemistry 84, 551-562. http://dx.doi.org/10.1016/S0308-8146(03)002784

Azlim AA, Ahmed JK, Syed ZI, Mustapha S, Asiyah MR, Kamarul R. 2010. Total phenolic content and primary antioxidant activity of methanolic extracts of aromatic plants’ leaves. International Food Research Journal 17, 1077-1084.

Bhaskar RD, Kiran CH, Madhavi Y, Rao PK, Rao TR. 2009. Evaluation of antioxidant potential of Clitoria ternatea L. and Eclipta prostrata L. International Journal of Biochemistry & Biophysics 46, 247-252.

Chanda S, Dave S, Kaneria M. 2011. In vitro antioxidant property of some Indian medicinal plants. Research Journal of Medicinal Plants 5(2), 169-179. http://dx.doi.org/10.3923/rjmp.2011.169.179

Chang SL, Chang CL, Chang YM, Hsieh RH, Tzeng CR, Wu TK, Shyur LF, Yang WC. 2004. Polyacetylenic compounds and butanol fraction from Bidenspilosa can modulate the differentiation of T-cells and prevent autoimmune diabetes in non-obese diabetic mice. Plants Med. 70 (11), 1045-1051.

Chipiti T, Ibrahim MA, Koorbanally NA, Islam MDS. 2015. In vitro antioxidant activity and GC-MS analysis of the ethanol and aqueous extracts of Cissus cornifolia (Baker) Splanch (Vitaceae) parts. Acta Poloniae Pharmaceutics-Drug Research 72, 119-127.

Dandekar R, Fegade R, Bhaskar VH. 2015. GC-MS analysis of phytoconstituents in alcohol extract of Epiphyllum oxypetalum leaves. Journal of Pharmacognosy and Phytochemistry 4(1), 149-154

Figueroa LV, Navarro LB, Vera MP, Petricevich VL. 2014. Antioxidant, total phenolic content and flavonoid contents, and cytotoxicity evaluation of Bougainvillea xbuttiana. International Journal of Pharmacy and Pharmaceutical Sciences 6(5), 498-502.

Formagio ASM, Volobuf CRF, Santiago M, Cardoso CAL, Vieira MdC, Pereira ZV. 2014. Evaluation of antioxidant activity, total flavonoids, tannins, and phenolic compounds in Psychotria leaf extracts. Antioxidants 3(4), 745-757. http://dx.doi.org/10.3390/antiox3040745

Garg R, Mohana DC, Manjunath K. 2013. Screening for Antioxidant activity in traditionally used medicinal herbs: Comparison of total phenols, radical scavenging and DNA damage protecting activity. International Journal of Pharma and Bio Sciences 4(2), 657 – 664.

Ghalot K, Lal V, Jha S. 2011. Phytochemical and pharmacological potential of Flemingia Roxb. Ex W.T. Aiton (Fabaceae). International Journal of Phytomedicine 2, 294-307.

Halliwell B. 1999. Antioxidant defence mechanisms: from the beginning to the end (of the beginning). Free Radical Research 31(4), 261–272. http://dx.doi.org/10.1080/10715769900300841

Hamidi N, Lazuoni HA, Moussaouie A, Ziane L, Amal S. 2012. GC-MS Analysis of ethanol extract from the aerial parts of Fagonialongispina (family zygophyllaceae). Asian Journal of Natural and Science 1, 136-142.

https://maps.google.com.ph/. 2015. Philippines. Retrieved from https://maps.google.com.ph/

Kazemi M. 2015. Phenolic profile, antioxidant capacity and anti-inflammatory activity of Anethum graveolens L. essential oil. Natural Product Research 29(6), 551-553. http://dx.doi.org/10.1080/14786419.2014.951934

Madan S, Singh G, Kumar Y, Kohli K, Singh R, Mir S, Ahmad S. 2008. A New Flavanone from Flemingia strobilifera (Linn) R.Br. and its antimicrobial activity. Tropical Journal of Pharmaceutical Research 7(1), 921-927. http://dx.doi.org/10.4314/tjpr.v7i1.14678.

Miguel MG. 2010. Antioxidant and anti-inflammatory activities of essential oils. A SHort Review. Molecules 15, 9252-9387.

Osawa T. 1994. Novel natural antioxidants for utilization in food and biological systems. In: Uritani I, Garcia VV, Mendoza EM, Eds. Postharvest biochemistry of plant food-materials in the tropics. Japan Scientific Societies Press, 241–251.

Othman A, Mukhtar NJ, Ismail NS, Changa SK. 2014. Phenolics, flavonoids content and antioxidant activities of 4 Malaysian herbal plants. International Food Research Journal 21(2), 759-766

Perera PRD, Ekanayake S, and Ranaweera KKDS. 2013. In vitro study on antiglycation activity, antioxidant activity and phenolic content of Osbeckia octandra L. leaf decoction. Journal of Pharmacognosy and Phytochemistry 2(4), 198-201.

Peteros NP, Uy M. 2010. Antioxidant and cytotoxic activities and phytochemical screening of four Philippine medicinal plants. Journal of Medicinal Plants Research 4(5), 407-414.

Prior RL, Wu X, Schaich K. 2005. Standardised methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. Journal of Agricultural and Food Chemistry 53(10), 4290-4302. http://dx.doi.org/10.1021/jf0502698

Ramcharan G, Clement Y, Maxwell A. 2010. Cytotoxic Activity of Selected West Indian Medicinal Plants against a Human Leukaemia Cell Line. West Indian Medical Journal 59(6), 597-601.

Rukaiyat M, Garva S, Labaran S. 2015. Antimicrobial activities of hexacosane isolated leaf from Sansveria liberica (Genome and Labroy) plant. Advancement in Medicinal Plant Research 3(3), 120-125.

Sanadhya I, Lobo V, Bhot M, Varghese J, Chandra N. 2013. In-vitro antioxidant activity of leaves of Anthocephalus indicus A. Rich. International Journal of Pharmacy and Pharmaceutical Sciences 5(2), 536-540.

Sermakanni M, Thangapandian V. 2012. GC-MS analysis of Cassia italica leaf methanol extract. Asian Journal of Pharmaceutical and Clinical Research 5(2), 90-94.

Singleton VL, Rossi JA Jr. 1965. Colorimetry of total phenolics with phosphomolybdic-phototungstic acid reagents. American Journal of Enology and Viticulture 16(3), 144-158.

Tahawa K, Alali FS, Gharaibeh M, Mohammad M, El-Elimat, T. 2007. Antioxidant activity and total phenolic content of selected Jordanian plant species. Food Chemistry 104(4), 1372-1378. http://dx.doi.org/10.1016/j.foodchem.2007.01.064

Vuong Q, Hirun S, Roach PD, Bowyera M, Phillips PA, Scarlett CJ. 2013. Effect of extraction conditions on total phenolic compounds and antioxidant activities of Carica papaya leaf aqueous extracts. Journal of Herbal Medicine 3(3), 104-111. http://dx.doi.org/110.1016/j.hermed.2013.04.004