GC-MS analysis of culture filtrates of Trichoderma harzianum and their efficacy of biocontrol measures of plant pathogens

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Research Paper 04/10/2024
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GC-MS analysis of culture filtrates of Trichoderma harzianum and their efficacy of biocontrol measures of plant pathogens

V. Manimegalai, V. Ambikapathy, A. Panneerselvam
Int. J. Biosci.25( 4), 60-67, October 2024.
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Abstract

Trichoderma species is a filamentous soil borne fungus known to be an effective bio control agent against plant pathogens. The chemical compositions of culture filtrates of Trichoderma harzianum were investigated using Perkin-Elmer Gas Chromatography–Mass Spectrometry, while the mass spectra of the compounds found in the extract was matched with the National Institute of Standards and Technology (NIST) library. Gas Chromatography – Mass Spectrometry analysis from T. harzianum showed five retention times 1.91, 4.28, 4.52, 26.42 and 30.21 min. There, action time 1.91 corresponds to 3,6,9,12,15,18-hexaoxa-1-triacontanolacid; 4.28corresponds to N- (tau-benzyl-N(1)-(tert-butoxycarbonyl) histidyl) serine methyl ester acid; 4.52 corresponds to didecyl phthalate acid; 4.22 corresponds to methoxy glutamic acid methyl ester; 4.32 corresponds to triacontanoic acid.

VIEWS 19

Afrasa M, Negussie M, Teshome T, Tesfaye A, Vetukuri RR. 2022. Antifungal compounds, GC-MS analysis and toxicity assessment of methanolic extracts of Trichoderma species in an animal model. PLoS One 17(9), e0274062.

Chet I. 1987. Trichoderma – application, mode of action and potential as a biocontrol agent of soil-borne plant pathogenic fungi. In: I. Chet (ed.), Innovative Approaches to Plant Disease Control, John Wiley and Sons: New York, pp. 137-160.

Cook RJ. 2000. Advances in plant health management in the 20th century. Annual Review of Phytopathology 38, 95-116.

Ellis MB. 1971. Dematiaceous Hyphomycetes. Kew, Surrey, England: Commonwealth Mycological Institute.

Ellis MB. 1976. More Dematiaceous Hyphomycetes. Kew, Surrey, England: Commonwealth Mycological Institute.

Garrett SD. 1956. Biology of root-infecting fungi. New York: Cambridge University Press.

Garrett SD. 1970. Pathogenic root-infecting fungi. New York: Cambridge University Press.

Gillman JC. 1957. A manual of soil fungi. Revised 2nd edition. Oxford and IBH Publishing Company, New Delhi, pp. 250-436.

Gilreath P. 2002. Manatee Vegetable Newsletter, University of Florida, Manatee County Extension Service, USA.

Haran S, Schinckler H, Chet I. 1996. Molecular mechanisms of lytic enzymes involved in the biological activity of Trichoderma harzianum. Microbiology 142, 2312-2331.

Harman GE, Hadar Y. 1983. Biological control of Phythium sp. Seed Science and Technology 11, 893-906.

Laszlo K, Rita B, Dora B, Henrietta A, Orsolya K, Gordana R, Andras V, Viktor DN, Csaba V, Gyorgy S. 2024. Recent advances in the use of Trichoderma-containing multicomponent microbial inoculants for pathogen control and plant growth promotion. World Journal of Microbiology and Biotechnology 40, 162.

Liu H, Jia W, Zhang J, Pan Y. 2007. GC-MS and GC-Olfactometry analysis of aroma compounds extracted from culture fluids of Antrodia camphorate. World Journal of Microbiology and Biotechnology.

Mukerji KG, Tewari JP, Arora DK, Saxena G. 1994. Studies on antagonistic interaction of some soil fungi against Fusarium moniliforme. Indian Journal of Botany Society 73, 265-267.

Mukherjee S, Tripathi HS. 2000. Biological and chemical control of wilt complex of French bean. Journal of Mycology and Plant Pathology 30(3), 380-385.

Naik NK, Sen B. 1992. Biocontrol of disease caused by Fusarium sp. In Recent Developments in Biocontrol of Plant Diseases.

Ngo BH, Vu DN, Tran DQ. 2006. Analyze antagonist effects of Trichoderma sp. for controlling southern stem rot caused by Sclerotium rolfsii on peanut. Plant Protection 1, 12-14.

Panneerselvam A, Saravanamuthu R. 1996. Antagonistic interaction of some soil fungi against Sarocladium oryzae. Indian Journal of Agricultural Research 30(1), 56-64.

Papavizas GC. 1985. Trichoderma and Gliocladium: Biology, ecology, and potential for biocontrol. Annual Review of Phytopathology 23, 23-54.

Prasad RD, Rangeshwaran P. 2001. Biological control of root and collar rot of chickpea caused by Sclerotium rolfsii. Annals of Plant Protection Science 34(2), 148-150.

Raper KB, Fennell DI. 1965. The genus Aspergillus. Baltimore: The Williams and Wilkins Co.

Raper KB, Thom C. 1949. A manual of Penicillia. Baltimore: The Williams and Wilkins Co.

Saikia MK, Gandhi SK. 2002. Comparative activities of three antagonist fungi against cauliflower stem rot pathogen Rhizoctonia solani. Journal of Mycology and Plant Pathology 33(1), 138-140.

Samuels GJ. 1996. Trichoderma: A review of biology and systematics of the genus. Mycological Research 100, 923-935.

Senthilkumar G, Madhanraj P, Panneerselvam A. 2011. Studies on the compounds and their antifungal potentiality of fungi isolated from paddy field soils of Jenbagapuram village, Thanjavur District, South India. Asian Journal of Pharmaceutical Research 1(1), 19-21.

Skidmore AM, Dickinson CM. 1976. Colony interaction and hyphal interferences between Septoria nodorum and phylloplane fungi. Transactions of the British Mycological Society 66, 57-64.

Stoppacher N, Kluger B, Zeilinger S, Krska R, Schuhmacher R. 2010. Identification and profiling of volatile metabolites of the biocontrol fungus Trichoderma viride by HS-SPME-GC-MS. Journal of Microbiological Methods 81(2), 187-193.

Subramanian CV. 1971. Hyphomycetes: An account of Indian species. Indian Council of Agricultural Research, New Delhi.

Ushadevi T. 2008. Studies on the microfungal in the Muthupet mangroves with emphasis on antimicrobial activity. PhD Thesis, Bharathidasan University, Trichirappalli, India.

Warcup JH. 1950. The soil plate method for isolation of fungi from soil. Nature 166, 117-118.

Wassima L, Ibtissem B, Mustapha MB, Hamdi B. 2023. Exploration and evaluation of secondary metabolites from Trichoderma harzianum: GC-MS analysis, phytochemical profiling, antifungal, and antioxidant activity assessment. Molecules 28(13), 5025.

Weindling R, Emerson H. 1936. The isolation of toxic substances from the culture filtrates of Trichoderma. Phytopathology 26, 1068-1070.

Xin ZH, Zhu WM, Gu RR. 2005. A new cytotoxic compound from Penicillium aurantiogriseum, symbiotic or epiphytic fungus of sponge Mycale plumosa. Chinese Chemical Letters 16, 1227-1229.

Yan L, Haiyan J, Xiuying Y. 2010. GC-MS analysis of fermentation fluid of Trichoderma sp. in rhizosphere soil of Populus euphratica. Journal of Northeast Forestry University 1(36).

Yassin MT, Mostafa AA-F, Al-Askar AA. 2021. Antagonistic activity of Trichoderma harzianum and Trichoderma viride strains against some fusarial pathogens causing stalk rot disease of maize in vitro. Journal of King Saud University Science 33(3), 101363.

Zin NA, Badaluddin NA. 2020. Biological functions of Trichoderma spp. for agriculture applications. Annals of Agricultural Sciences 65(2), 168-178.