Welcome to International Network for Natural Sciences | INNSpub

Paper Details

Research Paper | April 1, 2022

| Download 3

GC-MS based phytoconstituents profiling and phytochemical investigation of Annona muricata L.

H.S.Tambe, A.M. Bhosale, R.D. Borse, P.M. Dighe, S.L. Kakad

Key Words:

Int. J. Biosci.20(4), 50-58, April 2022

DOI: http://dx.doi.org/10.12692/ijb/20.4.50-58


IJB 2022 [Generate Certificate]


Annona muricata L. is conventionally used to treat various ailments. This plant shows varied medicinally valuable effects like anti-cancer, anti-hermitic, anti-spasmodic,anti-convulsant,anti-pyretic, sedative, hypotensive, digestive, anti-diabetes, anti-microbial,anti- inflammatory, anti- dysenteric, andanti- rheumatic effects. The phytochemical qualitative analysis of Annona muricata leaves exhibits the presence of carbohydrates, tannins, saponins, alkaloids, flavonoids, glycosides, quinines, phenols, terpenoids, coumarins, anthraquinones, steroids, phlobatannins and anthracyanine.The GC-MS analysis report shows the 22 compounds in the leaf ethanolic and hexane extract of Annona muricata by comparing retention time and interpretation of their mass spectra.


Copyright © 2022
By Authors and International Network for
Natural Sciences (INNSPUB)
This article is published under the terms of the Creative
Commons Attribution Liscense 4.0

GC-MS based phytoconstituents profiling and phytochemical investigation of Annona muricata L.

Aboul- Enein AM, Abu-Elalla F, Shalaby EA, El-Shemy HA. 2012. Traditional medicinal plants research in Egypt: Studies of anti-oxidant and anti-cancer activities. Journal of Medicinal Plant Research 6(5), 689-703. http://dx.doi.org/10.5897/JMPR11.968

Afify AM, Fayed SA, Shalaby EA, El-Shemy HA. 2011. Syzygiumcumini (pomposia) active principles exhibit potent anti-cancer and anti-oxidant activities. African Journal of Pharmacy and Pharmacology 5(7), 948-956. http://dx.doi.org/AJPP10.420

Alagammal M, Mohan VR, Paulpriya K. 2013. Anti-cancer activity of ethanol extract of polygala javana DC whole plant against Dalton Ascites Lymphoma. Research Journal of Recent Sciences. 2(2), 18-22.

Alamu O, Ofuya T, Oni MO, Idoke JE. 2020. Gas chromatography- mass spectrometry analysis of ethanol and ethyl acetate fractions of Annona muricata (L) seed oil. American Journal of Research Communication 8(11).

Cragg GM, Newman DJ, Snader KM. 1997. Natural products in drug discovery and development. Journal of Natural Products 60, 52-60. http://dx.doi.org/10.1021/np9604893

Das K, Tiwari RK, Shrivastava DK. 2010. Techniques for evaluation of medicinal plant products as antimicrobial agent: Current methods and future trends. Journal of Medicinal Plant Research 4(2). 104-111.

Doughari J, Human L, Bennade and Ndakidemi P. 2009. Phytochemicals as chemotherapeutic agents and anti-oxidants: Possible solution to the control of antibiotic resistant verocytotoxin producing bacteria. Journal of Medicinal Plant Research 3(11), 839-848.

Haddock RL. 1974. Some medicinal plants of Guam including English and Guamanian common names. Report Regional Tech Mtg Med Plants, Papeete. Tahiti, Nov, 1973; South Pacific Commision, Noumea,New Caledonia. 79.

Holdsworth DK. 1990. Traditional Medicinal Plants of Rarotonga. Cook Islands. Part I International Journal of Crude Drug Research. 28(3), 209-218. http://dx.doi.org/10.3109/13880209009082815

Joabe G, de-M, Araujo TAS, Castro VTNA, Cabral DLV, Rodrigues MD, Nascimento SC, Albuquerque UP. 2010. Antiproliferative activity, Anti-oxidant capacity and Tannin content in plants of semi-arid Northeastern Brazil. Molecules 15, 8534-8542. http://dx.doi.org/10.3390/molecules15128534

Krishnaiah D, Davi T, Bono A, Sarbatly R. 2009. Studies on phytochemical constituents of six Malaysian medicinal plants. Journal of Medicinal Plant Research 3(2), 67-72. http://dx.doi.org/

Kumar A, Ilavarasan R, Jayachandran T, Decaraman M, Aravindhan P, Padmanabhan P, Krishnam MR. 2009. Phytochemical investigation on a tropical plant, Syzygiumcumini from Kattuppalayam, Erode District, Tamil Nadu, South india. Pakistan Journal of Nutrition 81, 83-85. http://dx.doi.org/10.3923/pjn.2009.83.85

Lali Growther. 2018. Anti-cancer activity of Annona muricata leaf extract and screening for bioactive phytochemicals. International Journal of Pharmacy and Biological Sciences 8(1), 475-481.

Lans CA. 2006. Ethnomedicines used in Trinidad and Tobago for urinary problems and diabetes mellitus. Journal of Ethnobiology and Ethnomedicine 2, 45-55. http://dx.doi.org/10.1186/1746-4269-2-45

Morton JF. 1980. Caribbean and Latin American Folk Medicine and its Influence in the United States. Q. J. Crude Drug Research 18(2), 57-75.

Neergheen V, Bahorum T, Taylor E, Jen L, Aruma O. 2009. Targetting specific cell signaling transduction pathways by dietary and medicinal phytochemicals in cancer chemoprevention. Toxicology 278, 229-241. http://dx.doi.org/10.1016/j.tox.2009.10.010

Nermeen M, Arafa, Mahmoud Mouwad, Hattem M, El-Shabrawi. 2016. Comparison the organic and inorganic solvents effect on phenolic compounds extraction and the activity against breast carcinoma cell lines from callus cultures of Manihot esculenta. International Journal of Pharma Tech Research 9(12).

Nik NN, Harisum Y, Mohamad N. 2016. Acetogenins of Annona muricata leaves characterization & potential anti-cancer study. Integrative Cancer Science and Therapeutics 2056-4546. http://dx.doi.org/10.15761/ICST.1000202

Odeghe OB, Ezirim AU, Okochi VI, James AB, Adebeshi OA, Ogunnowo S. 2013. Induction of apoptosis in myelogenous leukemic k562 cells by ethanolic leaves extract of Annona muricata L. Global Journal of Research on Medicinal Plants & Indigenous Medicine 2(3), 143-144. http://dx.doi.org/

Orlando. 2010. Antinociceptive and Anti- Inflamatory activity activities of the ethanol extract of Annona muricata L. leaves in animal models. International Journal of Molecular Sciences 11(5). 2067-2078. http://dx.doi.org/10.3390/ijms11052067

Ovando-Dominguez MY, MC Lujan Hidalgo, Gonzalez- Mendoza D, Vargas-Diaz AA, Ruiz-Lau N, Gutierrez- Miceli FA, Lecona Guzman CA. 2019. Total phenols, flavonoids and antioxidants activity in Annona muricata and Annona purpurea callus culture. Tech Science Press. http://dx.doi.org/10.32604/phyton.2019.06546

Prashant Tiwari, Bimlesh Kumar, Mandeep Kaur, Gurpreet Kaur, Harleen Kaur. 2011. Phytochemical screening and extraction: A review. Internationale Pharmaceutica Sciencia 1(1), 98-106.

QorinaFona, Arsianti Ade, FithrotunnisalQotrunnada, TejaputriNadzilaAnindya, Azizah Norma Nur, RistaPutrianingsih. 2020. Cytotoxicity of soursop leaves (Annona muricata) against cervical Hela cancer cells. Pharmacognosy Journal 12(1), 20-24. http://dx.doi.org/10.5530/pj.2020.12.4

Rieser MJ, Fang XP, Rupprecht JK, Hui YH, Smith DL, McLaughlin JL. 1993. Bioactive single ring acetogenins from seed extract of Annona muricata. Planta Medica 59, 91-92. http://dx.doi.org/10.1055/s-2006-959614

Roghini R, Vijayalakshmi K. 2018. Phytochemical sceening, Quantitative analysis of flavonoids and minerals in ethanolic extract of Citrus paradise. International Journal of Pharmaceutical Sciences and Research 9(11), 4859-4864. http://dx.doi.org/10.13040/IJPSR.09758232.9(11).4859-64

Schultes RE, Raffauf. 1990. The healing forest: Medicinal and toxic plants of the Northwest Amazonia, Portland: R.F. Dioscorides Press. http://dx.doi.org/10.1017/S0376892900032641

Shibula K, Velavan S. 2015. Determination of phytoconstituents in methanolic extract of Annona muricata leaf using GC-MS technique. International Journal of Pharmacognosy and Phytochemical Research 7(6), 1251-1255.

Shukla Y, Pal SK. 2004. Dietary cancer chemoprevention; An overview. International Journal of Human Genetics 4, 265-276. http://dx.doi.org/10.1080/09723757.2004.11885905

Sies. 1993. Helmut. Strategies of antioxidant defense. European Journal of Biochemistry 215, 213-219. https://doi.org/10.1111/j.1432-1033.1993.tb18025.x

Syed Umar Faruq Syed Najmuddin Muhammad Firdaus Romli, Muhajir Hamid, Noorjahan Banu, Alitheen, Nik MohdAfizannik Abd Rahman. 2016. Anti-cancer effect of Annona muricata Linn leaves crude extract (AMCE) on breast cancer cell line. BMC Complementary and Alternative Medicine 16, 311. http://dx.doi.org/10.1186/s12906-016-1290-y

Taraphdar A, Madhurima R, Bhattacharya R. 2001. Natural products as inducers of apoptosis: Implication for cancer therapy and prevention. Current Science 80(11), 1387-1397.

Valko M, Leibfritz D, Moncol Jan Cronin MTD, Mazur M, Telser J. 2007. Free radicals andantioxidants in normal physiological functions and human disease. The International Journal of Biochemistry & Cell Biology 39, 44-84. http://dx.doi.org/10.1016/j.biocel.2006.07.001

Vijaymeena C, Subhashini G, Loganayagi M, Ramesh B. 2013. Phytochemicals screening and assessment of antibacterial activity for the bioactive compounds in Annona muricata. International Journal of Current Microbiology and Applied Sciences 2(1), 1-8.

Vinothini R, Lali Growther. 2016. Antimicrobial and Phytochemical Analysis of Methanolic and Aqueous Extract of Annona muricata (Leaf and Fruit). International Journal of Current Microbiology and Applied Sciences 5(10), 617-625. http://dx.doi.org/10.20546/ijcmas.2016.510.069

Wamidh HT. 2011. Anti-cancer and Antimicrobial potential of plant derived natural products. In: IrajRasooli, ed. Phytochemicals- Bioactivities and Impact on Health, Croatia, 141-158.

Wu FE, Gu ZM, Zeng L, Zhao GX, Zhang Y, Mclaughlin JL, Sastrodihardjo S. 1995. Two new cytotoxic monotetra hydrofuran annonaceous acetogenins, annomuricins A and B from the leaves of Annona muricata. Journal of Natural Products 58, 830-836. http://dx.doi.org/10.1021/np50120a002

Yahaya Gavamukulya, Faten Abou-Elella, Fred Wamunyokoli, Hany AEI-Shemy. 2014. Phytochemical screening, anti-oxidant activity and in vitro anti-cancer potential of ethanolic and water leaves extract of Annona muricata (Graviola). Asian Pacific Journal of Tropical Medicine 7(1), S355-S363. http://dx.doi.org/10.1016/S1995-7645(14)60258-3


Style Switcher

Select Layout
Chose Color
Chose Pattren
Chose Background