home icon user icon
Welcome to International Network for Natural Sciences | INNS Pub.
Home My Account
Submit Manuscript
double_slash
breadcumb_bg

General bioactivity screening of Homalocladium platycladum (Tapeworm plant) via brine shrimp lethality assay

Paper Details

Research Paper 13/06/2023
Views (353) Download (42)
current_issue_feature_image
publication_file

General bioactivity screening of Homalocladium platycladum (Tapeworm plant) via brine shrimp lethality assay

Javen Domugho, Janus B. Pansacala
Int. J. Biosci.22( 6), 189-197, June 2023.
Certificate: IJB 2023 [Generate Certificate]
Key: Bioactivity screeningBioassayBrine shrimpLC50Tapeworm plant

Abstract

This study aimed to evaluate the presence of bioactive compounds in Homalocladium platycladum (Tapeworm plant) through bioactivity screening and toxicity assessment. The powdered mature stems of the plant were extracted using different solvents, namely n-hexane (HEX), dichloromethane (DCM), ethyl acetate (ETA), n-butanol (BUT), and methanol-water (WAT). The resulting fractions were prepared and tested at various concentrations using the brine shrimp lethality assay. The results indicated that the HEX, DCM, and BUT fractions exhibited immediate and acute toxicity, suggesting the presence of bioactive compounds. Furthermore, all fractions demonstrated toxicity at longer exposure times, indicating the potential for chronic toxicity. The median lethal concentration (LC50) analysis using the Miller-Tainter method revealed moderate toxicity for the HEX and DCM fractions, with LC50 values of 199.9 ppm and 457.3 ppm, respectively. Moreover, all fractions, including HEX, DCM, ETA, BUT, and WAT, exhibited bioactivity potential at longer exposure times, with calculated LC50 values of 19.79 ppm, 38.82 ppm, 14.77 ppm, 5.565 ppm, and 131.3 ppm, respectively. Notably, all fractions, except for the methanol-water fraction, displayed high toxicity after 24-hour exposure. These findings suggest that the Tapeworm plant contains bioactive compounds with pharmacological potential. Therefore, further investigation of these compounds is warranted for the development of novel pharmaceutical agents.

VIEWS 110
Cover PDF

Abubakar AR, Haque M. 2020. Preparation of Medicinal Plants: Basic Extraction and Fractionation Procedures for Experimental Purposes. Journal of Pharmacy and Bioallied Sciences 12(1).

Arambasic M, Randhawa M. 2014. Comparison of the methods of Finney and Miller-Tainter for the calculation of LD50 values. World Appl. Sci. J 32(10), 2167-2170.

Azmir J, Zaidul ISM, Rahman MM, Sharif KM, Mohamed A, Sahena F, Jahurul MHA, Ghafoor K, Norulaini NAN, Omar AKM. 2013. Techniques for extraction of bioactive compounds from plant materials: A review. Journal of Food Engineering 117(4), 426-436.

Bernhoft A. 2010. Bioactive Compounds in Plants: Benefits and Risks for Man and Animals. In Det Norske Videnskaps-Akademi 2010. Volume 50, 7-255.

Dhawan D, Gupta J. 2016. Comparison of Different Solvents for Phytochemical Extraction Potential from Datura metel Plant Leaves. International Journal of Biological Chemistry 11, 17-22.

Finney DJ, Stevens WL. 1948. A Table for the Calculation of Working Probits and Weights in Probit Analysis. Biometrika 35(1/2), 191-201.

Hamidi M, Jovanova B, Kadifkova PT. 2014. Toxicological evaluation of the plant products using Brine Shrimp (Artemia salina L.) model. Macedonian Pharmaceutical Bulletin 60, 9-18.

Kemboi D, Kiplimo J, Denis K. 2016. Phytochemistry and antibacterial activity of extracts from medicinal plant Olea africana. African Journal of Pharmacy and Pharmacology 10, 330-336.

Krishnaraju AV, Rao TVN, Sundararaju D, Vanisree M, Tsay HS, Subbaraju GV. 2005. Assessment of Bioactivity of Indian Medicinal Plants Using Brine Shrimp (Artemia salina) Lethality Assay. International Journal of Applied Science and Engineering 3(2), 125-134.

Kupchan SM, Sigel CW, Guttman LJ, Restivo RJ, Bryan RF. 1972. Tumor inhibitors. LXXII. Datiscoside, a novel antileukemic cucurbitacin glycoside from Datisca glomerata. Journal of the American Chemical Society 94(4), 1353-1354.

Kupchan SM, Tsou G, Sigel CW. 1973. Datiscacin, a novel cytotoxic cucurbitacin 20-acetate from Datisca glomerata. The Journal of Organic Chemistry 38(7), 1420-1421.

Manfron J, Farago P, Duarte M, Takeda I. 2007. Morpho-anatomical study of the cladodes of Homalocladium platycladum (F.J. Muell.) L.H. Bailey (Polygonaceae). Revista Brasileira De Farmacognosia-Brazilian Journal of Pharmacognosy – 17.

Nuria M. 2010. Antibacterial activities from jangkang (Homalocladium platycladum (F. Muell) Bailey) leaves. Mediagro 6(2).

Otsuka H. 2008. Purification by Solvent Extraction Using Partition Coefficient. In Natural Products Isolation (pp. 269-273).

Randhawa M. 2009. Calculation of LD50 values from the method of Miller and Tainter, 1944. Journal of Ayub Medical College, Abbottabad : JAMC 21, 184-185.

Tiwari P, Kumar B, Kaur M, Kaur G, Kaur H. 2011. Phytochemical screening and Extraction: A Review. Internationale Pharmaceutica Scienca 1(1), 98-106

Submit Manuscript